Inter simple sequence repeats system optimization and fingerprinting for Longan

JIANG Fan; GAO Hui-ying; CHEN Xiu-ping; LI Tao; YANG Ling; ZHENG Shao-quan

Volume 22 Issue 3

Apr. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2007 > 22(3): 256-260

JIANG Fan, GAO Hui-ying, CHEN Xiu-ping, LI Tao, YANG Ling, ZHENG Shao-quan. Inter simple sequence repeats system optimization and fingerprinting for Longan[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 256-260.

Citation:

JIANG Fan, GAO Hui-ying, CHEN Xiu-ping, LI Tao, YANG Ling, ZHENG Shao-quan. Inter simple sequence repeats system optimization and fingerprinting for Longan[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 256-260.

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Inter simple sequence repeats system optimization and fingerprinting for Longan

1.
Fujian Breeding Engineering Technology Research Center for Longan and Loquat, Fuzhou, Fujian 350013, China;
2.
Pomology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
3.
Agricultural college, Hebei University of Engineering, Handan, Hebei 057150, China

Received Date: 2007-06-08
Rev Recd Date: 2007-08-10
Publish Date: 2007-09-15

Abstract

Abstract

Factors affecting the Inter-Simple Sequence Repeats (ISSR) analysis of longan,such as annealing temperature and concentrations of template DNA,Mg2 + and dNTP were examined.The optimal ISSR-PCR reaction system (25 μL) was found to be: 20 ng template DNA,1.5 U Taq DNA polymerase,0.7 μmol·L-1 primer,2.0 mmol·L-1 Mg2+,and 0.2 mmol·L-1 dNTPs.Using the single primer (ISSR-13),fingerprints of 12 genotypes were constructed from different origins with their abundant polymorphism of DNA spectra.The results suggested that ISSR-PCR could be a suitable reaction system for studying longan genetic diversity.
- Longan,
- ISSR-PCR,
- optimization,
- fingerprint

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References(5)

References

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