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Volume 21 Issue 3
Apr.  2012
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LIN Zheng-he, CHEN Rong-bing, CHEN Chang-song. Optimization of ISSR-PCR reaction system in tea plant[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 247-252.
Citation: LIN Zheng-he, CHEN Rong-bing, CHEN Chang-song. Optimization of ISSR-PCR reaction system in tea plant[J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 247-252.
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Optimization of ISSR-PCR reaction system in tea plant

  • Tea Research Institute, Fujian Academy of Agricultrual Sciences, Fu’an, Fujian 355014, China

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  • Received Date: February 13, 2006
  • Revised Date: April 20, 2006
    Graphical Abstract
    • Abstract
  • The optimal ISSR-PCR reaction conditions in tea was established by studying the main parameters.Results showed that the optimal concentration of DNA,Taq polymerase,primer,Mg2+,dNTp in 20 μl reaction mixture was 40 ng,0.5 U,0.25 μmol·L-1,1.5 mmol·L-1,0.2 mmol·L-1.The appropriate annealing temperature was 2-3℃ higher than Tm of corresponding primer.
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    • References (2)
  • 陈亮,陈大明,高其康,等.茶树基因组DNA提纯与鉴定[J].茶叶科学,1997,17(2):177-181.
    陈亮,高其康,杨亚军,等.茶树RAPD反应系统和扩增程序优化[J].茶叶科学,1998,18(1):16-20.
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