DNA Fingerprinting of 17 Dimocarpus longan Germplasms by Using ISSR Markers

ZHENG Shan; ZHANG Li-jie; ZHANG Xiao-yan; XIE Li-xue; LI Tao

doi:10.19303/j.issn.1008-0384.2012.12.008

Volume 27 Issue 12

Dec. 2012

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(12): 1308-1312

ZHENG Shan, ZHANG Li-jie, ZHANG Xiao-yan, XIE Li-xue, LI Tao. DNA Fingerprinting of 17 Dimocarpus longan Germplasms by Using ISSR Markers[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1308-1312. doi: 10.19303/j.issn.1008-0384.2012.12.008

Citation:

ZHENG Shan, ZHANG Li-jie, ZHANG Xiao-yan, XIE Li-xue, LI Tao. DNA Fingerprinting of 17 Dimocarpus longan Germplasms by Using ISSR Markers[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1308-1312. doi: 10.19303/j.issn.1008-0384.2012.12.008

Citation:

PDF( 1411 KB)

DNA Fingerprinting of 17 Dimocarpus longan Germplasms by Using ISSR Markers

doi: 10.19303/j.issn.1008-0384.2012.12.008

Fruit Research Institute,Fujian Academy of Agricultural Science,Fuzhou,Fujian 350003,China

Received Date: 2012-10-12
Publish Date: 2012-12-18

Abstract

Abstract

The inter-simple sequence repeat(ISSR) method was applied to assess the genetic makeup of 17,including 6 new longan varieties.Out of the 30 ISSR primers,5 were selected for the analysis.Cluster analysis showed that the genetic similarity coefficients ranged from 0.75 to 0.93,and that the 17 cultivars could be clustered into 4 groups using a genetic similarity coefficient threshold of 0.785.These varieties could be satisfactorily distinguished by a single ISSR primer,py-9,and established their digital DNA fingerprints accordingly.The results provided an important basis for identifying new longan varieties,as well as evaluating and utilizing the germplasm resources.
- Longan,
- ISSR,
- fingerprint,
- genetic diversity

FullText(HTML)

References(22)

References

[1]	郑少泉,姜帆,蒋际谋,等.高多糖龙眼杂交新品系“高宝”的选育[J].福建农业学报,2008,23(4):387-391.
[2]	PRRVOST A,WILKINSON M J.A new system of comparingPCR primers appliedto ISSR fingerprinting of potato cultivars[J].Theoretical and App1ied Genetics,1999,98:107-112.
[3]	王心宇,陈佩度,开增军,等.ISSR标记在小麦指纹图谱分析中的应用研究初探[J].农业生物技术学报,2001,9(3):261-263.
[4]	唐利华,肖扬,边银丙.中国黑木耳主要栽培菌株ISSR指纹分析及SCAR标记[J].菌物学报,2008,27(2):243-251.
[5]	董海燕.利用ISSR标记构建红花檵木品种(品系)指纹图谱[D].南京:南京林业大学,2009.
[6]	祁伟.应用ISSR与SRAP分子标记绘制红麻与蓖麻DNA指纹图谱[D].福州:福建农林大学,2008.
[7]	聂琼,刘仁祥,梁微.用ISSR标记构建烟草核心种质的指纹图谱[J].西南农业学报,2010,23(2):335-339.
[8]	陈虎,何新华,罗聪,等.龙眼24个品种的SCoT遗传多样性分析[J].园艺学报,2010,37(10):1651-1654.
[9]	袁磊.龙眼遗传多样性及亲缘关系的ISSR分析[D].南宁:广西大学,2007.
[10]	王莉,胡瑞法,黃季焜,等.大豆遗传多样性及其经济影响研究[J].中国农业科学,2001,34(6):604-609.
[11]	李海明,张世煌,胡瑞法.遗传单一性、品种单一性与中国玉米生产[J].浙江大学学报:农业与生命科学版,2005,31(6):677-682.
[12]	胡瑞法,黄季焜,Scott Rozelle.遗传单一性及其对中国小麦产量的影响[J].中国农业科学,2002,35(12):1442-1449.
[13]	李海明,胡瑞法.国外稻种资源对我国水稻生产的贡献[J].浙江大学学报:农业与生命科学版,2008,34(1):65-72.
[14]	滕海涛,吕波.利用DNA指纹图谱辅助植物新品种保护的可能性[J].生物技术通报,2009,(1):1-6.
[15]	TOMMASINI L,BATLEY J,ARNOLD G M,et al.Thedevelopment of multiplex simple sequence repeat(SSR)markers to complement distinctness,uniformity and stabilitytesting of rape(Brassica napus L.)varieties[J].Theoreticaland Applied Genetics,2003,106:1091-1101.
[16]	DE RIEK J,CALSYN E,EVERAERT I,et al.AFLP basedalternatives for the assessment of distinctness,uniformity andstability of sugar beet varieties[J].Theoretical and App1iedGenetics,2001,103:1254-1265.
[17]	张金渝,张建华,杨晓洪,等.玉米DUS测试标准品种的SSR分子指纹图谱的构建[J].玉米科学,2006,14(4):47-52.
[18]	王立新,常利芳,李宏博,等.小麦区试品系DUS测试的分子标记[J].作物学报,2010,36(7):1114-1125.
[19]	陆光远,伍晓明,张冬晓,等.SSR标记分析国家油菜区试品种的特异性和一致性[J].中国农业科学,2008,41(1):32-42.
[20]	刘丽娟,舒烈波,罗利军,等.SRAP标记与形态学标记在西瓜DUS测试中的比较[J].植物遗传资源学报,2011,12(5):790-795.
[21]	刘红艳,吴坤,杨敏敏,等.国家芝麻区域试验新品种(系)的DNA指纹分析[J].作物学报,2012,38(4):596-605.
[22]	张鹤,赵敬东,张文庆.DNA分子标记在植物新品种保护应用中存在的问题与对策[J].河南农业科学,2010,(1):129-130.