ISSR Fingerprints and Genetic Variations of Sun-Cured Tobacco Germplasms

QIN Jian-feng; HE Jiang; CHEN Tao; QIN Xu; CUI Ming-yong; HUANG Xian-ya; MENG Yao; LIANG Chun

doi:10.19303/j.issn.1008-0384.2019.05.004

Volume 34 Issue 5

Aug. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(5): 525-533

QIN Jian-feng, HE Jiang, CHEN Tao, QIN Xu, CUI Ming-yong, HUANG Xian-ya, MENG Yao, LIANG Chun. ISSR Fingerprints and Genetic Variations of Sun-Cured Tobacco Germplasms[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 525-533. doi: 10.19303/j.issn.1008-0384.2019.05.004

Citation:

QIN Jian-feng, HE Jiang, CHEN Tao, QIN Xu, CUI Ming-yong, HUANG Xian-ya, MENG Yao, LIANG Chun. ISSR Fingerprints and Genetic Variations of Sun-Cured Tobacco Germplasms[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 525-533. doi: 10.19303/j.issn.1008-0384.2019.05.004

Citation:

PDF( 1996 KB)

ISSR Fingerprints and Genetic Variations of Sun-Cured Tobacco Germplasms

doi: 10.19303/j.issn.1008-0384.2019.05.004

Guangxi Subtropical Crops Research Institute, Nanning, Guangxi 530001, China

Received Date: 2019-01-04
Rev Recd Date: 2019-03-18
Publish Date: 2019-05-28

Abstract

Abstract

Objective This study was aimed to accurately reveal the genetic relationship among sun-cured tobacco germplasms, and improve the utilization and breeding efficiency of sun-cured tobacco resources. Method Genetic diversity of 36 sun-cured tobacco germplasms was analyzed using ISSR. Result From the samples, 113 DNA bands were amplified with 7 polymorphic primers selected from 100 ISSR primers. Among them, 91 bands (80.5%) were polymorphic. The average effective number of alleles (Ne), Nei's gene diversity index (H), and Shannon's information index (I) of the ISSR markers were 1.64, 0.37, and 0.55, respectively, indicating a high detection efficacy. The UPGMA analysis showed a low diversity among the germplasms with the genetic similarity coefficients (GS) ranging from 0.74 to 0.91. With the new method, the germplasms were clustered into 5 groups at the level of 0.78.The digital fingerprints effectively distinguish the germplasms. Conclusion The ISSR markers chosen for the DNA fingerprints of 36 sun-cured tobacco germplasms were appropriate for the otherwise difficult task of differentiating and identifying those genetically closely related varieties.
- sun-cured tobacco,
- ISSR,
- fingerprint maps,
- genetic diversity

FullText(HTML)

References(20)

References

[1]	杨友才, 周清明, 尹晗琪.烟草RAPD反应体系的建立与优化研究[J].中国农学通报, 2005, 21(5):97-100. doi: 10.3969/j.issn.1000-6850.2005.05.029 YANG Y C, ZHOU Q M, YIN H Q.Establishment and Optimization of RAPD Reaction System in Tobacco[J].Chinese Agricultural Science Bulletin, 2005, 21(5):97-100.(in Chinese) doi: 10.3969/j.issn.1000-6850.2005.05.029
[2]	杨友才, 周清明, 尹晗琪.利用RAPD和AFLP标记分析烟草种质资源的遗传多样性[J].农业生物技术学报, 2006, 14(4):585-593. doi: 10.3969/j.issn.1674-7968.2006.04.029 YANG Y C, ZHOU Q M, YIN H Q. Analysis of Genetic Diversity in Tobacco Germplasm by RAPDs and AFLPs[J].Journal of Agricultural Biotechnology, 2006, 14(4):585-593.(in Chinese) doi: 10.3969/j.issn.1674-7968.2006.04.029
[3]	肖炳光.利用RAPD和ISSR标记分析烤烟品种间遗传关系[J].武汉植物学研究, 2006, 24(5):392-396. doi: 10.3969/j.issn.2095-0837.2006.05.002 XIAO B G.Assessment of Genetic Relationships between the F lue-cured TobaccoVarieties by RAPD and ISSR Markers[J].Journal of Wuhan Botanical Research, 2006, 24(5):392-396.(in Chinese) doi: 10.3969/j.issn.2095-0837.2006.05.002
[4]	杨友才, 余硕辉, 尹晗琪.48个烟草品种遗传多样性的RAPD分析[J].亚热带植物科学, 2008, 37(3):10-14. doi: 10.3969/j.issn.1009-7791.2008.03.003 YANG Y C, YU S H, YIN H Q.Studies on Genetic Diversity of 48 Tobacco Varieties by RAPD Analysis[J].Subtropical Plant Science2008, 37(3):10-14.(in Chinese) doi: 10.3969/j.issn.1009-7791.2008.03.003
[5]	郭金平, 朱惠丽, 周以飞, 等.部分烤烟种质遗传多样性与亲缘关系的RAPD分析[J].中国烟草科学, 2009, 30:15-18, 24. http://d.old.wanfangdata.com.cn/Periodical/zgyckx2009z1003 GUO J P, ZHU H L, ZHOU Y F, et al. Genetic Diversity and Genetic Relationship Analysis of Some Flue-curedTobacco Germplasms Based on RAPD[J].Chinese Tobacco Science, 2009, 30:15-18, 24.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgyckx2009z1003
[6]	王慧颖, 王曼, 朴世领, 等.晒烟种质资源形态学标记及SSR标记的多样性分析[J].湖北农业科学, 2015, 54(23):5943-5948. http://d.old.wanfangdata.com.cn/Periodical/hbnykx201523038 WANG H Y, WANG M, PIAO S L, et al. The Genetic Diversity Analyses on Sun-cured Tobacco Germplasm by Morphological Markers and SSR Markers[J].Hubei Agricultural Sciences, 2015, 54(23):5943-5948.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnykx201523038
[7]	张雪廷, 童治军, 焦芳婵, 等.38份晾晒烟种质资源遗传关系的SSR分析[J].植物遗传资源学报, 2013, 14(4):653-658. http://d.old.wanfangdata.com.cn/Periodical/zwyczyxb201304012 ZHANG X T, TONG Z J, JIAO F C, et al.Genetic Relationship Analysis of Thirty-eight Sun/Air-Cured TobaccoGermplasms Based on Simple Sequence Repeat (SSR) Markers[J].Journal of Plant Genetic Resources, 2013, 14(4):653-658.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwyczyxb201304012
[8]	陈杰, 杨静, 龙胜贤, 等.SSR分子标记在烟草研究中的应用进展[J].生物技术通报, 2015, 31(3):43-48. http://d.old.wanfangdata.com.cn/Periodical/swjstb201503006 CHEN J, YANG J, LONG S X, et al.Advance of the Application of SSR Molecular Markers in Tobacco Research[J].Biotechnology Bulletin, 2015, 31(3):43-48.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/swjstb201503006
[9]	刘林, 罗文学, 官思成, 等.用SSR标记揭示部分烤烟种质资源的遗传差异[J].四川农业大学学报, 2018, 36(2):153-160. http://d.old.wanfangdata.com.cn/Periodical/scnydxxb201802005 LIU L, LUO W X, GUAN S C, et al.Accessment of Genetic Difference among Some Flue-cured Tobacco Germplasms Using SSR Markers[J].Journal of Sichuan Agricultural University, 2018, 36(2):153-160.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/scnydxxb201802005
[10]	龙腾, 刘雷, 黄玉碧.四川部分晾晒烟种质遗传关系的SRAP分析[J].作物学报, 2009, 35(1):173-178. http://d.old.wanfangdata.com.cn/Periodical/zuowxb200901023 LONG T, LIU L, HUANG Y B.Genetic Analysis of Sichuan Sun-Cured Tobacco Germplasm by SRAP[J].Acta Agronomica Sinica, 2009, 35(1):173-178.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb200901023
[11]	王日新, 任民, 贾兴华, 等.烟草主要栽培类型的SRAP标记研究[J].生物技术通报, 2009(6):100-104. http://d.old.wanfangdata.com.cn/Periodical/swjstb200906023 WANG R X, REN M, JIA X H, et al.Study of SRAP Markers in Major Cultivated Forms of Nicotiana tabacum L.[J].Biotechnology Bulletin, 2009(6):100-104.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/swjstb200906023
[12]	祁建民, 梁景霞, 陈美霞, 等.应用ISSR与SRAP分析烟草种质资源遗传多样性及遗传演化关系[J].作物学报, 2012, 38(8):1425-1434. http://d.old.wanfangdata.com.cn/Periodical/zuowxb201208010 QI J M, LIANG J X, CHEN M X, et al.Genetic Diversity and Evolutionary Analysis of Tobacco (Nicotiana tabacum L.)Germplasm Resources Based on ISSR and SRAP Markers[J].Acta Agronomica Sinica, 2012, 38(8):1425-1434.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb201208010
[13]	张吉顺, 张孝廉, 杨春元, 等.地方晒烟种质资源SRAP标记的遗传多样性分析[J].分子植物育种, 2016, 14(7):1906-1913. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201607038 ZHANG J S, ZHANG X L, YANG C Y, ,et al.The Genetic Diversity Analysis of Sun-cured Tobacco Landraces Germplasms by SRAP Markers[J].Molecular Plant Breeding, 2016, 14(7):1906-1913.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201607038
[14]	刘超, 党江波, 魏烨昕, 等.SCoT分子标记技术初步应用于烟草属部分材料的遗传分析及种间杂种的鉴定[J].中国烟草学报, 2013, 19(5):107-111. doi: 10.3969/j.issn.1004-5708.2013.05.019 LIU C, DANG J B, WEI Y X, et al.The application of SCoT molecular marker technology ingenetic analysis and interspecifc hybrid identifcation in genus Nicotiana[J].Acta Tabacaria Sinica, 2013, 19(5):107-111.(in Chinese) doi: 10.3969/j.issn.1004-5708.2013.05.019
[15]	王建波.ISSR分子标记及其在植物遗传学研究中的应用[J].遗传学报, 2002, 24(5):613-616. http://d.old.wanfangdata.com.cn/Periodical/yc200205022 WANG J B.ISSR Markers and Their Application in Plant Genetic[J].Hereditas(Beijing), 2002, 24(5):613-616.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yc200205022
[16]	覃剑峰, 何江, 王新钧, 等.烟草ISSR-PCR反应体系优化的初实验[J].农业研究与应用, 2018, 31(3):19-22. doi: 10.3969/j.issn.2095-0764.2018.03.004 QIN J F, HE J, WANG X J, et al Preliminary experiment on Optimization of ISSR-PCR Reaction System for Tobacco[J].Agricultural Research and Application.2018, 3:19-22.(in Chinese) doi: 10.3969/j.issn.2095-0764.2018.03.004
[17]	杨祥燕, 蔡元保, 黄秋伟, 等.番木瓜主栽品种SCoT指纹图谱构建及遗传变异分析[J].西北植物学报, 2013, 33(9):1756-1761. http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201309007 YANG X Y, CAI Y B, HUANG Q W, et al.SCoT Fingerprints and Genetic Variations of the Papaya(Carica papaya L.)Major Cultivars[J].Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(9):1756-1761.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201309007
[18]	陈真勇, 彭正松, 陈卫英.四川晾晒烟的ISSR分析[J].西华师范大学学报(自然科学版), 2012, 33(2):154-160. http://d.old.wanfangdata.com.cn/Periodical/scsfxyxb-zr201202009 CHEN Z Y, PANG Z S, CHEN W Y.Genetic Analysis of Sichuan Sun-Cured Tobacco Germplasm by ISSR[J]Journal of China West Normal University (Natural Sciences), 2012, 33(2):154-160.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/scsfxyxb-zr201202009
[19]	尚志强.分子标记技术在烟草种质资源研究中的应用进展[J].生物技术通报, 2010(3):58-61. http://d.old.wanfangdata.com.cn/Periodical/swjstb201003013 SHANG Z Q.Progress of the Application of Molecular Markers in Tobacco Research Related to Germplasm Resource[J].Biotechnology Bulletin, 2010(3):58-61.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/swjstb201003013
[20]	陈杰, 杨静, 郭鸿雁, 等.DNA分子标记技术在烟草遗传育种中的应用[J].中国农学通报, 2012, 28(7):95-99. doi: 10.3969/j.issn.1000-6850.2012.07.018 CHEN J, YANG J, GUO H Y, et al.Advance of Molecular Marker and Its Application in the Tobacco Genetic Breeding[J].Chinese Agricultural Science Bulletin, 2012, 28(7):95-99.(in Chinese) doi: 10.3969/j.issn.1000-6850.2012.07.018