Identification of Pathogen and Biocontrol Agents on Southern Blight of <i>Pseudostellaria heterophylla</i>

CHEN Yanping; XIAO Rongfeng; JIN Guo; CHEN Meichun; ZHENG Xuefang; ZHU Yujing; LIU Bo; WANG Jieping

doi:10.19303/j.issn.1008-0384.2021.10.012

Volume 36 Issue 10

Oct. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(10): 1203-1209

CHEN Y P, XIAO R F, JIN G, et al. Identification of Pathogen and Biocontrol Agents on Southern Blight of Pseudostellaria heterophylla [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1203−1209 doi: 10.19303/j.issn.1008-0384.2021.10.012

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CHEN Y P, XIAO R F, JIN G, et al. Identification of Pathogen and Biocontrol Agents on Southern Blight of Pseudostellaria heterophylla [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1203−1209 doi: 10.19303/j.issn.1008-0384.2021.10.012

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Identification of Pathogen and Biocontrol Agents on Southern Blight of Pseudostellaria heterophylla

doi: 10.19303/j.issn.1008-0384.2021.10.012

1.
Institute of Agricultural Bio-Resources Research, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Agricultural and Rural Bureau of Zherong, Ningde, Fujian　355300, China

Received Date: 2021-07-31
Accepted Date: 2021-07-31
Rev Recd Date: 2021-09-22

Available Online: 2021-10-23

Publish Date: 2021-10-28

Abstract

Abstract

Objective Pathogen and potential biocontrol agents of the southern blight, a major soil-borne disease, of Pseudostellaria heterophylla were identified. Method P. heterophylla plant parts and tubers showing the typical southern blight symptoms were collected for the pathogen isolation. The isolate was examined by morphological, molecular biological, and pathogenicity tests for taxonomic confirmation. Microbes with an antibacterial property against the isolate were screened using the plate confrontation culture method to evaluate the potential of their being used as a biocontrol agent on the blight. Result On culture plate, the round colonies of the suspected pathogen had white, silky, spreading mycelia with septa and ramifications that, at later stage, formed sclerotia in different colors. The isolate was further identified as Athelia rolfsii by ITS, LSU, and TEF-1α gene sequence analyses. The disease symptoms on the plants inoculated with the isolate were identical to those observed in the field and in accordance with Koch's rule. On the biocontrol efficacy evaluation, Bacillus velezensis FJAT-17931 and B. siamensis FJAT-52595 displayed high inhibitory effect against A. rolfsii with the rates of 72.23 % and 71.16%, respectively. Conclusion The pathogen of the southern blight on P. heterophylla was identified as A. rolfsii. Two Bacillus strains were found to be potentially applicable as biocontrol agents on the disease that can cause serious yield loss on the crop.
- Pseudostellaria heterophylla,
- southern blight,
- Athelia rolfsii,
- identification,
- biocontrol agent

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References

[1]	江维克, 周涛. 太子参产业发展现状及其建议 [J]. 中国中药杂志, 2016, 41(13):2377−2380. JIANG W K, ZHOU T. Pesudostellariae Radix industry status and development countermeasures [J]. China Journal of Chinese Materia Medica, 2016, 41(13): 2377−2380.(in Chinese)
[2]	肖荣凤, 陈燕萍, 陈梅春, 等. 太子参根腐病病原菌的鉴定及防治药剂筛选 [J]. 植物保护学报, 2020, 47(6):1333−1342. XIAO R F, CHEN Y P, CHEN M C, et al. Pathogen identification of root rot of Pseudostellaria heterophylla plant and fungicide screening for its efficient control [J]. Journal of Plant Protection, 2020, 47(6): 1333−1342.(in Chinese)
[3]	曾桂萍, 赵致, 李忠. 贵州太子参白绢病发生及防治 [J]. 耕作与栽培, 2020(6):37−38,41. ZENG G P, ZHAO Z, LI Z. The occurrence and prevention of Pseudostellaria heterophylla southern blight in Guizhou [J]. Tillage and Cultivation, 2020(6): 37−38,41.(in Chinese)
[4]	桑维钧, 熊继文, 宋宝安, 等. 贵州太子参主要真菌病害的调查与防治 [J]. 安徽农业科学, 2006, 34(14):3314−3316,3318. doi: 10.3969/j.issn.0517-6611.2006.14.083 SANG W J, XIONG J W, SONG B A, et al. Investigation of main fungus diseases of ginseng in Guizhou Province [J]. Journal of Anhui Agricultural Sciences, 2006, 34(14): 3314−3316,3318.(in Chinese) doi: 10.3969/j.issn.0517-6611.2006.14.083
[5]	周丹, 程搏幸, 丁波. 太子参真菌病害和防治研究进展 [J]. 中国植保导刊, 2020, 40(9):23−27,32. doi: 10.3969/j.issn.1672-6820.2020.09.004 ZHOU D, CHENG B X, DING B. Research progress on fungal diseases of Pseudostellaria heterophylla and their control [J]. China Plant Protection, 2020, 40(9): 23−27,32.(in Chinese) doi: 10.3969/j.issn.1672-6820.2020.09.004
[6]	陈桂华. 太子参白绢病药剂防治效果试验 [J]. 内蒙古农业科技, 2010, 38(2):54−54,57. CHEN G H. Control effect of Pseudostellaria heterophylla southern blight with fungicides [J]. Inner Mongolia Agricultural Science and Technology, 2010, 38(2): 54−54,57.(in Chinese)
[7]	姜星, 祝前, 刘德江. 太子参白绢病田间药效试验初报[J]. 农业与技术, 2017, 37(18): 23. JIANG X, ZHU Q, LIU D J. Preliminary report on field efficacy test of white blight of Pseudostellaria heterophylla. Agriculture & Technology, 2017, 37(18): 23. (in Chinese)
[8]	陈凤毛. 真菌ITS区序列结构及其应用 [J]. 林业科技开发, 2007, 21(2):5−7. doi: 10.3969/j.issn.1000-8101.2007.02.002 CHEN F M. The sequence of ITS region and its application of fungus [J]. China Forestry Science and Technology, 2007, 21(2): 5−7.(in Chinese) doi: 10.3969/j.issn.1000-8101.2007.02.002
[9]	HARLTON C E. Genetic diversity in Sclerotium (Athelia) rolfsii and related species [J]. Phytopathology, 1996, 85(10): 1269.
[10]	肖仲久, 李小霞, 蒋选利. 贵州省白术白绢病菌遗传多样性评价 [J]. 热带作物学报, 2012, 33(9):1686−1689. doi: 10.3969/j.issn.1000-2561.2012.09.029 XIAO Z J, LI X X, JIANG X L. Genetic diversity analysis of Sclerotium rolfsii Sacc. in rhizoma of Atractylodes macrocephala [J]. Chinese Journal of Tropical Crops, 2012, 33(9): 1686−1689.(in Chinese) doi: 10.3969/j.issn.1000-2561.2012.09.029
[11]	郭艳春, 姚嘉瑜, 张镕斌, 等. 中国黄麻炭疽病病原菌的分离鉴定及系统发育分析 [J]. 作物学报, 2021(8):1−17. GUO Y C, YAO J Y, ZHANG R B, et al. Identification and phylogenetic analysis of the pathogen of jute anthracnose in China [J]. Acta Agronomica Sinica, 2021(8): 1−17.(in Chinese)
[12]	ALPER I, FRENETTE M, LABRIE S. Ribosomal DNA polymorphisms in the yeast Geotrichum candidum [J]. Fungal Biology, 2011, 115(12): 1259−1269. doi: 10.1016/j.funbio.2011.09.002
[13]	CHEN Q, LI J, MIAO Y, et al. First report of southern blight on Chrysanthemum morifolium caused by Sclerotium rolfsii in china [J]. Plant Disease, 2019, 104(2): 310.
[14]	陈婧, 桑维钧. 道真玄参白绢病病原鉴定及杀菌剂毒力测定 [J]. 湖北农业科学, 2015, 54(20):5020−5021,5024. CHEN J, SANG W J. Identification and fungicide toxicity determination of the Sclerotium rolfsii Sacc. on Scrophularia ningpoensis Hemsl. in Daozhen [J]. Hubei Agricultural Sciences, 2015, 54(20): 5020−5021,5024.(in Chinese)
[15]	张佳星, 徐颖菲, 徐艳芳, 等. 白术两种主要土传病害的分离、鉴定及杀菌剂的室内活性筛选 [J]. 植物保护, 2017, 43(6):177−181,186. doi: 10.3969/j.issn.0529-1542.2017.06.031 ZHANG J X, XU Y F, XU Y F, et al. Isolation and identification of two main soil-borne pathogens on Atractylodes macrocephala and screening of fungicides in vitro [J]. Plant Protection, 2017, 43(6): 177−181,186.(in Chinese) doi: 10.3969/j.issn.0529-1542.2017.06.031
[16]	王国荣, 沈伟东, 孙超, 等. 铁皮石斛茎基部2种主要病害病原菌的分离与鉴定 [J]. 植物保护, 2017, 43(1):168−172. doi: 10.3969/j.issn.0529-1542.2017.01.030 WANG G R, SHEN W D, SUN C, et al. Isolation and identification of two main basal stem pathogens on Dendrobium officinale [J]. Plant Protection, 2017, 43(1): 168−172.(in Chinese) doi: 10.3969/j.issn.0529-1542.2017.01.030
[17]	崔月贞, 郝兴顺, 魏芳勤, 等. 秦巴山区魔芋白绢病病原菌的鉴定及生物学特性的测定 [J]. 江西农业学报, 2020, 32(4):103−107. CUI Y Z, HAO X S, WEI F Q, et al. Identification and biological characteristics of Hypochnus centrifugus in Qinba Mountains area [J]. Acta Agriculturae Jiangxi, 2020, 32(4): 103−107.(in Chinese)
[18]	金苹, 高晓余. 白绢病的研究 [J]. 农业灾害研究, 2011, 1(1):14−22. JIN P, GAO X Y. Research and overview of southern blight [J]. Journal of Agricultural Catastrophology, 2011, 1(1): 14−22.(in Chinese)
[19]	NIE Q, ZHENG T, LIU L, et al. First report of southern blight on Pinellia ternata caused by Athelia rolfsii in china [J]. Plant Disease, 2020, 104(5): 11.
[20]	KAMIL D, BAHADUR A, DEBNATH P, et al. First report of leaf sheath rot of Areca nut (Arecacatechu) caused by Athelia rolfsii from tripura, india [J]. Plant Disease, 2020, 104(11): 1−2.
[21]	SEO Y, PARK M J, BACK C G, et al. First report of stem rot caused by Athelia rolfsii on Zamioculcas zamiifolia in korea [J]. Plant Disease, 2019, 103(11): 1−4.
[22]	LI J, CHEN Q, WANG M, et al. First report of southern blight on cynanchum stauntonii caused by Athelia rolfsii in china [J]. Plant Disease, 2020, 104(6): 1−2.
[23]	XU Z H, HARRINGTON T C, GLEASON M L, et al. Phylogenetic placement of plant pathogenic sclerotium species among teleomorph genera [J]. Mycologia, 2010, 102(2): 337−346. doi: 10.3852/08-189
[24]	刘思睿. 贵州省太子参主要根部病害病原鉴定及防治[D]. 贵阳: 贵州大学, 2020. LIU S R. Identification and control of main root diseases of Pseudostellariae in Guizhou province[D]. Guiyang: Guizhou University, 2020. (in Chinese)
[25]	张金凤, 成波, 曹延丽, 等. 花生白绢病生防菌筛选及防控作用研究 [J]. 花生学报, 2019, 48(3):65−70. ZHANG J F, CHENG B, CAO Y L, et al. Screening and controlling effect of biocontrol bacteria against peanut white blight [J]. Journal of Peanut Science, 2019, 48(3): 65−70.(in Chinese)
[26]	张献强, 黎起秦, 甘启范, 等. 防治茉莉白绢病的药剂筛选研究 [J]. 安徽农业科学, 2010, 38(10):5152−5154,5228. doi: 10.3969/j.issn.0517-6611.2010.10.077 ZHANG X Q, LI Q Q, GAN Q F, et al. Screening fungicides for controlling jasmine Sclerotium rolfsii Blight [J]. Journal of Anhui Agricultural Sciences, 2010, 38(10): 5152−5154,5228.(in Chinese) doi: 10.3969/j.issn.0517-6611.2010.10.077