• 中文核心期刊
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Volume 22 Issue 3
Apr.  2012
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Abstract
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LIN Jie, LIN Zhi-min, SONG Ya-na, HUAN Yue-ying, CHEN Jun. PCR and amplification of ITS and 5.8S ribosomal DNA for Fusarium genus detection and identification[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 241-244.
Citation: LIN Jie, LIN Zhi-min, SONG Ya-na, HUAN Yue-ying, CHEN Jun. PCR and amplification of ITS and 5.8S ribosomal DNA for Fusarium genus detection and identification[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 241-244.
shu

PCR and amplification of ITS and 5.8S ribosomal DNA for Fusarium genus detection and identification

  • 1.

    Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;

  • 2.

    Plant Protection and Quarantine Station, Agricultural Department of Fujian Province, Fuzhou, Fujian 350003, China

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  • Received Date: July 09, 2007
  • Revised Date: August 20, 2007
    Graphical Abstract
    • Abstract
  • Internal transcribed spacer 1 (ITS1),ITS2 and 5.8S rDNA were amplified by PCR and semi-nested PCR to detect Fusarium DNA.Meanwhile, PCR and semi-nested PCR of the region were employed to evaluate the sensitivity and specificity of the method.The amplified DNA was sequenced and blasted against sequences in the GenBank at the National Center for Biotechnology Information.The results proved that this DNA amplifying technique was able to distinguish different species of Fusarium genus.Therefore,amplifying and sequencing of ITS/5.8S rDNA could be a rapid,applicable method for identifying Furisarum genus.
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    • References (5)
  • 杨红,李颖,关国华,等.尖孢镰刀菌异核体及其不同核型分离子rDNAITS区序列分析[J].农业生物技术学报,2002,10(4):381-384.
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    LEES S B,TAYLOR J W.Phylogeny of five fungus-like protocfistan Phytophthora species,inferred from theinernal transcribed spacers ofriboso mal DNA[J].Mol Biol Evol,1992,(9):636-653.
    HIBBETT D S.RIBOSOMAL.RNA and fungal systematics[J].Trans.Mycol.Soc,1992,33:533-556.
    杜道林,马文儒,苏杰,等.SDS、CTAB和PVP法提取香蕉基因组DNA的比较研究[J].海南师范学院学报:自然科学版,2003,16(1):74-80.
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