PCR and amplification of ITS and 5.8S ribosomal DNA for <i>Fusarium</i> genus detection and identification

LIN Jie; LIN Zhi-min; SONG Ya-na; HUAN Yue-ying; CHEN Jun

Volume 22 Issue 3

Apr. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2007 > 22(3): 241-244

LIN Jie, LIN Zhi-min, SONG Ya-na, HUAN Yue-ying, CHEN Jun. PCR and amplification of ITS and 5.8S ribosomal DNA for Fusarium genus detection and identification[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 241-244.

Citation:

LIN Jie, LIN Zhi-min, SONG Ya-na, HUAN Yue-ying, CHEN Jun. PCR and amplification of ITS and 5.8S ribosomal DNA for Fusarium genus detection and identification[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 241-244.

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PCR and amplification of ITS and 5.8S ribosomal DNA for Fusarium genus detection and identification

1.
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Plant Protection and Quarantine Station, Agricultural Department of Fujian Province, Fuzhou, Fujian 350003, China

Received Date: 2007-07-10
Rev Recd Date: 2007-08-21
Publish Date: 2007-09-15

Abstract

Abstract

Internal transcribed spacer 1 (ITS1),ITS2 and 5.8S rDNA were amplified by PCR and semi-nested PCR to detect Fusarium DNA.Meanwhile, PCR and semi-nested PCR of the region were employed to evaluate the sensitivity and specificity of the method.The amplified DNA was sequenced and blasted against sequences in the GenBank at the National Center for Biotechnology Information.The results proved that this DNA amplifying technique was able to distinguish different species of Fusarium genus.Therefore,amplifying and sequencing of ITS/5.8S rDNA could be a rapid,applicable method for identifying Furisarum genus.
- rDNA sequence,
- fungal pathogens,
- semi-nested PCR

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References(5)

References

[1]	杨红,李颖,关国华,等.尖孢镰刀菌异核体及其不同核型分离子rDNAITS区序列分析[J].农业生物技术学报,2002,10(4):381-384.
[2]	BRUNS T D,WHITE T J,Taylor J W.Ftm galmolecular system atics[J].Annu Rev Ecol Syst,1991,22:525-564.
[3]	LEES S B,TAYLOR J W.Phylogeny of five fungus-like protocfistan Phytophthora species,inferred from theinernal transcribed spacers ofriboso mal DNA[J].Mol Biol Evol,1992,(9):636-653.
[4]	HIBBETT D S.RIBOSOMAL.RNA and fungal systematics[J].Trans.Mycol.Soc,1992,33:533-556.
[5]	杜道林,马文儒,苏杰,等.SDS、CTAB和PVP法提取香蕉基因组DNA的比较研究[J].海南师范学院学报:自然科学版,2003,16(1):74-80.