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Volume 26 Issue 6
Feb.  2012
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SUN Da-guang, ZHENG Xue-fang, LIU Bo, RUAN Chuan-qing, XIA Yu-lu, DUAN Yong-ping. Identification and 16S rDNA Sequence Analysis of Citrus Huanglongbing Pathogen[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 1027-1033.
Citation: SUN Da-guang, ZHENG Xue-fang, LIU Bo, RUAN Chuan-qing, XIA Yu-lu, DUAN Yong-ping. Identification and 16S rDNA Sequence Analysis of Citrus Huanglongbing Pathogen[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 1027-1033.
shu

Identification and 16S rDNA Sequence Analysis of Citrus Huanglongbing Pathogen

  • 1.

    College of Life Science Fujian Normal University,Fuzhou,Fujian 350108,China;

  • 2.

    Citrus Huanglongbing Research Center,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350003,China;

  • 3.

    The Center of Integrated Pest Management, North Carolina State University,North Carolina 27606,USA;

  • 4.

    Horticultural Research Laboratory (USDA/ARS),Florida 34945,USA

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  • Received Date: October 31, 2011
  • Revised Date: November 25, 2011
    Graphical Abstract
    • Abstract
  • Nested-PCR detection of Shunchang citrus Huanglongbing pathogen was carried out in this study.Phylogenetic tree based on rDNA sequencing was built to find out the evolutionary between different parts citrus HLB pathogens.Digesting the target DNA fragment of 16S rDNA with restriction enzyme XbaI was carried out to confirm the species of Shunchang Huanglongbing pathogen.The results showed that there were 7 positive detections in 12 selected samples, and the rate of positive detections was 58.33%,and four were dominant character,three were recessive character.The results showed that Shunchang citrus Huanglongbing pathogen belong to Candidatus Liberibacter asiaticus based on the 16S rDNA phylogenetic analysis and restriction enzyme XbaI digestion analysis.And 16S rDNA sequence gap analysis showed that the difference between Ca. L.asiaticus and Ca. L.africanus, Ca. L.africanus and Ca.L.americanus, Ca.L.americanus and Ca.L.asiaticus were 2.75%,3.90%, 3.44%, respectively.
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    • References (7)
  • 廖晓兰,朱水芳,赵文军,等. 柑桔黄龙病病原 16SrDNA 克隆,测序及实时荧光 PCR 检测方法的建立[J]. 农业生物技术学报,2004,12(1):80-85.
    刘福明,张纯胄,李妙寿,等. 温州柑橘缺素症调查,鉴别与防治技术研究[J]. 浙江柑橘,2006,23(2): 12-13.
    林秀萍,柑桔黄龙病综合防控技术[J]. 果农之友,2007,(2): 29-30.
    TOMIMURA K,MIYATA S,FURUYA N,et al. Evaluation of Genetic Diversity Among Candidatus Liberibacter asiaticus Isolates Collected in Southeast Asia[J]. Phytopathology,2009,99(9):1062-1069.
    GARNIER M,JAGOUEIX-EVEILLARD S,CRONJE P R,et al. Genomic characterization of a liberibacter present in an ornamental rutaceous tree,Calodendrum capense,in the Western Cape Province of South Africa. Proposal of Candidatus Liberibacter africanus subsp.capensis [J]. International Journal of Systematic and Evolutionary Microbiology,2000,50(6):2119-2125.
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