- 中文核心期刊
- CSCD来源期刊
- 中国科技核心期刊
- CA、CABI、ZR收录期刊
| Citation: |
GUAN X F, CHEN S Y, LAI G T, et al. Optimization of Astaxanthin-producing Fermentation by Phaffia rhodozyma using Okara as Nitrogen Source [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1102−1110. DOI: 10.19303/j.issn.1008-0384.2024.09.012
|
An astaxanthin-producing fermentation by Phaffia rhodozyma using okara for nitrogen was optimized.
On the conventional fermentation by P. rhodozyma to make astaxanthin, okara was used to replace the commonly applied peptone and yeast extract as the organic nitrogen source for cost reduction. Effects of carbon sources, precursor substances, other nitrogen supply, vitamins, and inorganic salts on yield of astaxanthin were analyzed with the amounts of (NH4)2SO4, vitamin E, glucose, and sucrose optimized by response surface methodology.
When okara was used as a raw ingredient for the fermentation, glucose became the optimal carbon source. The yield of astaxanthin by the P. rhodozyma fermentation could be significantly increased by applying both glucose and sucrose, potassium salts such as KCl, KNO3, and K2HPO4 as well as (NH4)2SO4, VB2, VE, and zeaxanthin. Hence, the medium was optimized by response surface method on the 4 key ingredients of glucose, sucrose, K2SO4, and VE to arrive at a formulation consisting of 10% okara, 0.22% K2SO4, 0.6% VE, 1.08% glucose, and 1.50% sucrose to reach a yield of astaxanthin at 32.46 mg·L−1, which was 2.23 folds higher than what obtained by using the YM medium.
Okara could amply be used to replace peptone and yeast extract as the nitrogen source for the astaxanthin production by P. rhodozyma fermentation.
| [1] |
王宝贝, 凌雪萍, 郑宗宇, 等. 维生素对红法夫酵母产虾青素的影响 [J]. 厦门大学学报(自然科学版), 2011, 50(1):111−116.
WANG B B, LING X P, ZHENG Z Y, et al. Effect of vitamins on astaxanthin production of Phaffia rhodozyma [J]. Journal of Xiamen University (Natural Science), 2011, 50(1): 111−116. (in Chinese)
|
| [2] |
周桂雄, 王闻, 谭雪松, 等. 利用农业废弃物碳源的红法夫酵母生产虾青素研究进展 [J]. 农业工程学报, 2016, 32(15):308−314. DOI: 10.11975/j.issn.1002-6819.2016.15.043
ZHOU G X, WANG W, TAN X S, et al. Review on astaxanthin production from agricultural wastes by Phaffia rhodozyma [J]. Transactions of the Chinese Society of Agricultural Engineering, 2016, 32(15): 308−314. (in Chinese) DOI: 10.11975/j.issn.1002-6819.2016.15.043
|
| [3] |
LAI J X, LIU W P, BU J, et al. Enhancement of astaxanthin production from food waste by Phaffia rhodozyma screened by flow cytometry and feed application potential [J]. Biotechnology and Applied Biochemistry, 2023, 70(6): 1817−1829. DOI: 10.1002/bab.2484
|
| [4] |
张辰, 徐慧, 朱坤福, 等. 微生物法生产虾青素的研究进展 [J]. 中国酿造, 2021, 40(10):29−35. DOI: 10.11882/j.issn.0254-5071.2021.10.005
ZHANG C, XU H, ZHU K F, et al. Research progress in the production of astaxanthin by microbial method [J]. China Brewing, 2021, 40(10): 29−35. (in Chinese) DOI: 10.11882/j.issn.0254-5071.2021.10.005
|
| [5] |
HIGUERA-CIAPARA I, FÉLIX-VALENZUELA L, GOYCOOLEA F M. Astaxanthin: A review of its chemistry and applications [J]. Critical Reviews in Food Science and Nutrition, 2006, 46(2): 185−196. DOI: 10.1080/10408690590957188
|
| [6] |
ELBAHNASWY S, ELSHOPAKEY G E. Recent progress in practical applications of a potential carotenoid astaxanthin in aquaculture industry: A review [J]. Fish Physiology and Biochemistry, 2024, 50(1): 97−126. DOI: 10.1007/s10695-022-01167-0
|
| [7] |
NAIR A, AHIRWAR A, SINGH S, et al. Astaxanthin as a king of ketocarotenoids: Structure, synthesis, accumulation, bioavailability and antioxidant properties [J]. Marine Drugs, 2023, 21(3): 176. DOI: 10.3390/md21030176
|
| [8] |
林嘉祺, 庄岩, 刘骁, 等. 一株海洋红法夫酵母Phaffia rhodozyma RP-306产虾青素的发酵条件优化 [J]. 海洋科学, 2024, 48(2):69−78.
LIN J Q, ZHUANG Y, LIU X, et al. Optimization of fermentation conditions for astaxanthin production by marine Phaffia rhodozyma RP-306 [J]. Marine Sciences, 2024, 48(2): 69−78. (in Chinese)
|
| [9] |
潘雪珊, 凌雪萍, 叶驰名, 等. 红发夫酵母生产虾青素的氮源补加策略 [J]. 厦门大学学报(自然科学版), 2013, 52(4):545−552.
PAN X S, LING X P, YE C M, et al. Nitrogen feeding strategies on astaxanthin production by Xanthophyllomyces dendrorhous [J]. Journal of Xiamen University (Natural Science), 2013, 52(4): 545−552. (in Chinese)
|
| [10] |
安君. 红法夫酵母发酵钝顶螺旋藻合成虾青素及载虾青素微粒功能性质研究[D]. 北京: 北京林业大学, 2020.
AN J. Study on synthesis of astaxanthin from Spirulina platensis by Phaffia rhodozyma and functional properties of astaxanthin-loaded particles[D]. Beijing: Beijing Forestry University, 2020. (in Chinese)
|
| [11] |
江文涛, 彭立影, 马加军, 等. 产虾青素红法夫酵母的发酵工艺优化 [J]. 饲料博览, 2021(12):13−18. DOI: 10.3969/j.issn.1001-0084.2021.12.003
JIANG W T, PENG L Y, MA J J, et al. Optimization of fermentation process of astaxanthin producing Phaffia rhodozyma [J]. Feed Review, 2021(12): 13−18. (in Chinese) DOI: 10.3969/j.issn.1001-0084.2021.12.003
|
| [12] |
张晶. 红法夫酵母菌株筛选及TiO2胁迫提高其虾青素产量的作用机制[D]. 长春: 吉林农业大学, 2022.
ZHANG J. Screening of Phaffia rhodozyma strain and the mechanism of Increasing Its astaxanthin production under TiO2 stress[D]. Changchun: Jilin Agricultural University, 2022. (in Chinese)
|
| [13] |
祝义伟, 龙勃, 龙勇, 等. 豆渣中营养成分的检测及其含量声称 [J]. 食品研究与开发, 2017, 38(8):117−120. DOI: 10.3969/j.issn.1005-6521.2017.08.027
ZHU Y W, LONG B, LONG Y, et al. Determination and content claim of nutrients in okara [J]. Food Research and Development, 2017, 38(8): 117−120. (in Chinese) DOI: 10.3969/j.issn.1005-6521.2017.08.027
|
| [14] |
韩扬, 何聪芬, 董银卯, 等. 响应面法优化超声波辅助酶法制备燕麦ACE抑制肽的工艺研究 [J]. 食品科学, 2009, 30(22):44−49. DOI: 10.3321/j.issn:1002-6630.2009.22.006
HAN Y, HE C F, DONG Y M, et al. Response surface optimization of ultrasonic-assisted enzymatic preparation of ACE inhibitory peptides from oat [J]. Food Science, 2009, 30(22): 44−49. (in Chinese) DOI: 10.3321/j.issn:1002-6630.2009.22.006
|
| [15] |
倪辉, 何国庆, 杨远帆, 等. 法夫酵母虾青素提取工艺的优化研究 [J]. 农业工程学报, 2004, 20(2):204−208. DOI: 10.3321/j.issn:1002-6819.2004.02.049
NI H, HE G Q, YANG Y F, et al. Optimization of condition for extracting astaxanthin from Phaffia rhodozyma [J]. Transactions of the Chinese Society of Agricultural Engineering, 2004, 20(2): 204−208. (in Chinese) DOI: 10.3321/j.issn:1002-6819.2004.02.049
|
| [16] |
STOKLOSA R J, JOHNSTON D B, NGHIEM N P. Phaffia rhodozyma cultivation on structural and non-structural sugars from sweet Sorghum for astaxanthin generation [J]. Process Biochemistry, 2019, 83: 9−17. DOI: 10.1016/j.procbio.2019.04.005
|
| [17] |
MIAO L L, CHI S, WU M R, et al. Deregulation of phytoene-β-carotene synthase results in derepression of astaxanthin synthesis at high glucose concentration in Phaffia rhodozyma astaxanthin-overproducing strain MK19 [J]. BMC Microbiology, 2019, 19(1): 133. DOI: 10.1186/s12866-019-1507-6
|
| [18] |
王严飞, 刘燕青, 陶正国, 等. 玉米黄素转化为虾青素的工艺参数研究 [J]. 天然产物研究与开发, 2014, 26(2):278−282.
WANG Y F, LIU Y Q, TAO Z G, et al. Study on technical parameters of converting Zeaxanthin into astaxanthin [J]. Natural Product Research and Development, 2014, 26(2): 278−282. (in Chinese)
|
| [19] |
刘春利, 沈宁燕, 倪辉, 等. 乙醇对法夫酵母发酵合成虾青素的影响 [J]. 食品与发酵工业, 2018, 44(3):1−7.
LIU C L, SHEN N Y, NI H, et al. Effect of ethanol on synthesis of astaxanthin by Phaffia rhodozyma [J]. Food and Fermentation Industries, 2018, 44(3): 1−7. (in Chinese)
|
| [20] |
YAMANE Y, HIGASHIDA K, NAKASHIMADA Y, et al. Astaxanthin production by Phaffia rhodozyma enhanced in fed-batch culture with glucose and ethanol feeding [J]. Biotechnology Letters, 1997, 19(11): 1109−1111. DOI: 10.1023/A:1018492611011
|
| [21] |
黎丽, 窦光鹏, 霍文严, 等. 高产虾青素红法夫酵母的选育和工艺优化 [J]. 中国食品添加剂, 2014, 25(9):140−146. DOI: 10.3969/j.issn.1006-2513.2014.09.014
LI L, DOU G P, HUO W Y, et al. Screening and optimization of a high-yield astaxanthin-producing Phaffia rhodozyma [J]. China Food Additives, 2014, 25(9): 140−146. (in Chinese) DOI: 10.3969/j.issn.1006-2513.2014.09.014
|
| [22] |
王雪, 孙美娟, 刘军贤, 等. 激光镊子拉曼光谱法优化红法夫酵母合成虾青素条件 [J]. 光散射学报, 2013, 25(2):152−157. DOI: 10.3969/j.issn.1004-5929.2013.02.009
WANG X, SUN M J, LIU J X, et al. Condition optimization of astaxanthin production in Phaffia rhodozyma using laser tweezers Raman spectroscopy [J]. The Journal of Light Scattering, 2013, 25(2): 152−157. (in Chinese) DOI: 10.3969/j.issn.1004-5929.2013.02.009
|
| [23] |
徐建春, 孙翰, 张睿钦, 等. 响应面分析法优化雨生红球藻产虾青素培养基 [J]. 中国酿造, 2014, 33(12):72−75. DOI: 10.11882/j.issn.0254-5071.2014.12.014
XU J C, SUN H, ZHANG R Q, et al. Optimization of Haematococcus Pluvialis medium producing astaxanthin by response surface methodology [J]. China Brewing, 2014, 33(12): 72−75. (in Chinese) DOI: 10.11882/j.issn.0254-5071.2014.12.014
|
| [1] | WANG Jianchao, HE Yinying, HUANG Xuping, SHEN Chaogui, CHEN Faxing, Guo Linrong. Response Surface Optimization of ISSR-PCR Reaction for Genetic Study on Phyllanthus Emblica[J]. Fujian Journal of Agricultural Sciences, 2021, 36(7): 759-765. DOI: 10.19303/j.issn.1008-0384.2021.07.004 |
| [2] | HUANG Qiong, HE Yan-ping. Optimization of Ultrasound/Microwave-assisted Extraction of Polysaccharides from Hibiscus sabdariffa L. by Box-Behnken Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1324-1329. DOI: 10.19303/j.issn.1008-0384.2018.12.016 |
| [3] | XIAO Zhi-yong. Response Surface Optimization of Muskmelon Fruit Fermentation for Vinegar[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 980-985. DOI: 10.19303/j.issn.1008-0384.2018.09.016 |
| [4] | WANG Hong-li, LIANG Zhang-cheng, HE Zhi-gang, LIN Xiao-zi, LIN Xiang-yang, CHEN Shen. Response Surface Optimization of Ca2+ Treatment for Texture Improvement on Kelp Products[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 631-637. DOI: 10.19303/j.issn.1008-0384.2018.06.016 |
| [5] | HE Shu-lan, ZHU Fu-rong, ZHU Hong-yang, RAO Xue-e, LUO Wen-fang. Optimization of Flavonoids Extraction from Leaves of Helianthus tuberosus L. by Response Surface Methodology and Determination of Antimicrobial Activity of Resulting Extract[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 897-904. DOI: 10.19303/j.issn.1008-0384.2017.08.017 |
| [6] | ZENG Zhi-heng, CHENG Yi, ZENG Hui, YANG Lei, LIAO Jian-hua, SHI Xiao-kun, CAI Zhi-ying, DAI Jian-qing. Optimization by Response Surface Methodology for Agaricus bisporus W38 Fermentation in Liquid Medium[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 161-166. DOI: 10.19303/j.issn.1008-0384.2017.02.011 |
| [7] | WU Hong-qiang, WANG Juan-juan, ZHUANG Wei-jing, ZHENG Ya-feng, ZHANG Yi. Optimization of Flavonoid Extraction from Ganoderma lucidum by Response Surface Methodology and Antioxidant Activities of Extracts[J]. Fujian Journal of Agricultural Sciences, 2017, 32(1): 87-92. DOI: 10.19303/j.issn.1008-0384.2017.01.018 |
| [8] | DANG Xiao-yan, WANG Yin, SU Yong-chang, LIU Shu-ji, WU Cheng-ye, CHEN Li-jiao. Response Surface Optimization of Enzymatic Extraction Loach Polysaccharides[J]. Fujian Journal of Agricultural Sciences, 2016, 31(7): 752-757. DOI: 10.19303/j.issn.1008-0384.2016.07.017 |
| [9] | LI Long, SU Yong-chang, WU Cheng-ye. EDTA-Na_2 Extraction of Agarose with Response Surface Optimization[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 158-165. DOI: 10.19303/j.issn.1008-0384.2013.02.014 |
| [10] | LIU Qiu-feng, SU Yong-chang, WU Cheng-ye. Optimization of Agaropectin Extraction with Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 1004-1010. |
| 1. |
贺望兴，谢小群，李延升，李文金，杨普香，江新凤，江亮辉，张春云. 2株生防菌与苏云金杆菌混配对茶园扁刺蛾的防治效果研究. 江西农业学报. 2024(05): 79-83 .
| |
| 2. |
江宏燕，陈世春，廖姝然，陈亭旭，杨普香，谢小群，王晓庆. 扁刺蛾线粒体基因组全序列特征及系统发育分析. 茶叶科学. 2023(04): 460-472 .
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: