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Volume 27 Issue 9
Nov.  2012
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LIU Qiu-feng, SU Yong-chang, WU Cheng-ye. Optimization of Agaropectin Extraction with Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 1004-1010.
Citation: LIU Qiu-feng, SU Yong-chang, WU Cheng-ye. Optimization of Agaropectin Extraction with Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 1004-1010.
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Optimization of Agaropectin Extraction with Response Surface Methodology

  • 1.

    College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;

  • 2.

    Fishries Research Institute of Fujian, Xiamen, Fujian 361013, China

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  • Received Date: June 24, 2012
  • Revised Date: July 09, 2012
    Graphical Abstract
    • Abstract
  • Agaropectin was extracted by EDTA-Na2, the content of sulfate group and total sugar were determined by barium sulfate turbidimetry and hydrochloric acid and phenol-sulfuric acid method, respectively. Based on single factor experiments, the extraction technique for agaropectin was optimized using response surface methodology, the mathematical regression model was established for predicting the value of sulfate group content plus total sugar for agaropectin (Y) at different levels of adding EDTA-Na2(A), treating time (B) and temperature (C). The optimal extraction parameters for agaropectin were determined as: adding EDTA-Na2 5.90 g·L-1, treating time 2.2 h at temperature of 53℃, respectively. With the optimal parameters, the content of sulfate group plus total sugar for agaropectin extracted was 84.00±0.04%, which was in good agreement with the predicted value(Y).
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    • References (3)
  • 慕运动. 响应面方法及其在食品工业中的应用[J]. 郑州工程学院学报,2001,22(3):91-94.
    TUULIKKI T,TERHO K,HARTIALA.Method for determination of the sulfatecontent of glycosaminoglycans[J]. Analytical Biochemistry,1971,41(2):471-476.
    曲有乐,李明,李宪刚,等.海藻多糖片中多糖含量测定[J].浙江海洋学院学报:自然科学版,2009,28(1):91-98.
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