Optimization of Ultrasound/Microwave-assisted Extraction of Polysaccharides from <i>Hibiscus sabdariffa</i> L. by Box-Behnken Response Surface Methodology

HUANG Qiong; HE Yan-ping

doi:10.19303/j.issn.1008-0384.2018.12.016

Volume 33 Issue 12

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(12): 1324-1329

HUANG Qiong, HE Yan-ping. Optimization of Ultrasound/Microwave-assisted Extraction of Polysaccharides from Hibiscus sabdariffa L. by Box-Behnken Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1324-1329. doi: 10.19303/j.issn.1008-0384.2018.12.016

Citation:

HUANG Qiong, HE Yan-ping. Optimization of Ultrasound/Microwave-assisted Extraction of Polysaccharides from Hibiscus sabdariffa L. by Box-Behnken Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1324-1329. doi: 10.19303/j.issn.1008-0384.2018.12.016

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Optimization of Ultrasound/Microwave-assisted Extraction of Polysaccharides from Hibiscus sabdariffa L. by Box-Behnken Response Surface Methodology

doi: 10.19303/j.issn.1008-0384.2018.12.016

Fujian Vocational College of Agriculture, Fuzhou, Fujian 350003, China

Received Date: 2018-09-16
Rev Recd Date: 2018-11-18
Publish Date: 2018-12-28

Abstract

Abstract

The extraction of polysaccharides from Hibiscus sabdariffa L. utilizing both ultrasound and microwave was optimized by Box-Behnken response surface methodology. For the single factor experiment the independent variables were the solvent-substrate ratio, extraction time and microwave power applied for the process. The resulting polysaccharides yields were compared among the various processes including the water, microwave-assisted, ultrasound-assisted, and ultrasonic/microwave-assisted extractions. The combination of ultrasonic and microwave was found to improve the efficiency and time-saving for the process. It was, therefore, further optimized using the Box-Behnken response surface method to finalize the extraction that required a solvent-substrate ratio of 20:1 (mL:g), an extraction duration of 15min, and a combined application of 150 W microwave power and 240 W ultrasound to reach a 3.51% polysaccharide yield from H. sabdariffa.
- Box-Behnken response surface methodology,
- ultrasound-microwave combination,
- Hibiscus sabdariffa L.,
- polysaccharides,
- extraction

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References(21)

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