- 中文核心期刊
- CSCD来源期刊
- 中国科技核心期刊
- CA、CABI、ZR收录期刊
| Citation: |
QIN Q H，HUANG H H，JIN M，et al. Species, Growth-promoting, and Disease-resistance of Antagonistic Microbes in Passion Fruit Rhizosphere Soil[J]. Fujian Journal of Agricultural Sciences，2025，40（2） ：189−197. DOI: 10.19303/j.issn.1008-0384.2025.02.009
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Microorganisms antagonistic to Fusarium solani were isolated from the rhizosphere soil of healthy passion fruit plants and studied for potential application as a biocontrol agent.
Rhizosphere oil at the field of healthy passion fruit plants was sampled to isolate microbes antagonistic to F. solani using the confrontational culture method. Morphological observation and molecular identification were performed to determine the species and biological characteristics of the isolates. Potential as a biocontrol agent of the identified strains was evaluated on the extracellular enzyme detection plates, while possible growth promoting effect examined on tomato seeds.
Eight microbial strains antagonistic to F. solani were isolated from 7 soil specimens. Among them, NN-Q03-10, SL-Q02-05, and SL-Q02-06 exhibited the greatest inhibition rates of 57.81%, 57.34%, and 57.34%, respectively. Four of them belonged to Bacillus subtilis, and 4 to B. velezensis. All could produce proteases, cellulases, β-1,3-glucanases, and xylanases with genomes consisting of antibiotic bacA, FenB, and yanJ. In addition to those genes, SL-Q02-05, NN-Q03-01, NN-Q03-09, and NN-Q03-10 also contained MycB and ifuA.Validation of growth promoting effects using tomato seeds, and the results showed that six strains (SL-Q02-04、SL-Q02-05、SL-Q02-06、SL-Q03-01、NN-Q03-01、NN-Q03-10) could promote embryonic root growth, among them, SL-Q02-04 had the most significant promoting effect with a embryonic root length of 7.32 cm.
The 8 Bacillus strains antagonistic to F. solani were identified. Their ability in producing protease, cellulase, β-1,3-glucanase, and xylanase and some of them having genomes containing mycB, ifuA, FenB, bacA, and yndJ related to antibiotic property strongly suggested a potential in serving as a biocontrol agent for F. solani infection on plants.
| [1] |
滕峥,杨翠凤,林有林. 西番莲茎基腐病主要致病菌及其防治研究进展[J]. 安徽农学通报,2021,27(20) :100−102.
TENG Z,YANG C F,LIN Y L. Research progress on main pathogenic bacteria of stem-base rot of Passiflora coerulea L. and its control[J]. Anhui Agricultural Science Bulletin,2021,27(20) :100−102. (in Chinese)
|
| [2] |
陈圆,赵志祥,严婉荣,等. 百香果茎基腐病病原鉴定及室内毒力测定[J]. 中国南方果树,2022,51(6) :90−94.
CHEN Y,ZHAO Z X,YAN W R,et al. Identification of stem base rot pathogen of passion fruit and its indoor toxicity test[J]. South China Fruits,2022,51(6) :90−94. (in Chinese)
|
| [3] |
SHAFI J,TIAN H,JI M S. Bacillusspecies as versatile weapons for plant pathogens:A review[J]. Biotechnology & Biotechnological Equipment,2017,31(3) :446−459.
|
| [4] |
陈学新,杜永均,黄健华,等. 我国作物病虫害生物防治研究与应用最新进展[J]. 植物保护,2023,49(5) :340−370.
CHEN X X,DU Y J,HUANG J H,et al. Recent progresses in biological control of crop pathogens and insect pests in China[J]. Plant Protection,2023,49(5) :340−370. (in Chinese)
|
| [5] |
许沛冬,易剑锋,陈迪,等. 贝莱斯芽孢杆菌生防次级代谢产物研究进展[J]. 生物技术通报,2024,40(3) :75−88.
XU P D,YI J F,CHEN D,et al. Research progress in the biocontrol secondary metabolites of Bacillus velezensis[J]. Biotechnology Bulletin,2024,40(3) :75−88. (in Chinese)
|
| [6] |
DE MORAES A C P,DA SILVA RIBEIRO L,DE CAMARGO E R,et al. The potential of nanomaterials associated with plant growth-promoting bacteria in agriculture[J]. 3 Biotech,2021,11(7) :318. DOI: 10.1007/s13205-021-02870-0
|
| [7] |
WANG C C,YE X J,NG T B,et al. Study on the biocontrol potential of antifungal peptides produced by Bacillus velezensis against Fusarium solani that infects the passion fruit Passiflora edulis[J]. Journal of Agricultural and Food Chemistry,2021,69(7) :2051−2061. DOI: 10.1021/acs.jafc.0c06106
|
| [8] |
CHEN Y H,LEE P C,HUANG T P. Biological control of collar rot on passion fruits via induction of apoptosis in the collar rot pathogen by Bacillus subtilis[J]. Phytopathology,2021,111(4) :627−638.
|
| [9] |
LIU Y F,CHEN Z Y,NG T B,et al. Bacisubin,an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916[J]. Peptides,2007,28(3) :553−559. DOI: 10.1016/j.peptides.2006.10.009
|
| [10] |
田凤鸣,陈强,何九军,等. 一株花椒根腐病拮抗菌的筛选、发酵条件及其抑菌物质的初步分析[J]. 微生物学通报,2023,50(7) :2950−2969.
TIAN F M,CHEN Q,HE J J,et al. A biocontrol bacterial strain against Zanthoxylum bungeanum root rot:Screening,fermentation condition optimization,and preliminary identification of antimicrobial ingredients[J]. Microbiology China,2023,50(7) :2950−2969. (in Chinese)
|
| [11] |
RATNANINGSIH H R,NOVIANA Z,DEWI T K,et al. IAA and ACC deaminase producing-bacteria isolated from the rhizosphere of pineapple plants grown under different abiotic and biotic stresses[J]. Heliyon,2023,9(6) :e16306. DOI: 10.1016/j.heliyon.2023.e16306
|
| [12] |
杨东亚,祁瑞雪,李昭轩,等. 黄瓜茄病镰刀菌拮抗芽胞杆菌的筛选、鉴定及促生效果[J]. 生物技术通报,2023,39(2) :211−220.
YANG D Y,QI R X,LI Z X,et al. Screening,Identification and Growth-promoting Effect of Antagonistic Bacillus spp. Against Cucumber Fusarium solani[J]. Biotechnology Bulletin,2023,39(2) :211−220. (in Chinese)
|
| [13] |
李丽丽,曾广宇,王继春,等. 油茶根腐病内生拮抗促生细菌筛选及诱导系统抗性研究[J]. 中国油料作物学报:2024,46(6) :1390−1397.
LI L L,ZENG G Y,WANG J C,et al. Screening and systematic resistance induction of endogenous antagonistic growth-promoting bacteria against camellia oleifera root rot[J]. Chinese Journal of Oil Crop Sciences:2024,46(6) :1390−1397. (in Chinese)
|
| [14] |
尚笑男,刘君昂,冯福山,等. 油茶内生拮抗细菌的筛选、鉴定及防效[J]. 中国生物防治学报,2021,37(3) :575−583.
SHANG X N,LIU J A,FENG F S,et al. Screening,identification and antagonistic effect of endophytic antagonistic bacterial strains from Camellia oleifera[J]. Chinese Journal of Biological Control,2021,37(3) :575−583. (in Chinese)
|
| [15] |
兰成忠,甘林,代玉立,等. 黄瓜枯萎病菌拮抗菌的筛选、鉴定和防效测定[J]. 中国生物防治学报,2023,39(1) :184−193.
LAN C Z,GAN L,DAI Y L,et al. Screening,identification and biocontrol effect of antagonistic strain against Fusarium oxysporum f. sp. cucumerinum[J]. Chinese Journal of Biological Control,2023,39(1) :184−193. (in Chinese)
|
| [16] |
黄秀梨. 微生物学实验指导[M]. 北京:高等教育出版社,1999.
|
| [17] |
魏靖宇,韩雨桐,翟文旭,等. 黄瓜土传病害拮抗细菌的筛选、鉴定及生防特性研究[J]. 中国生物防治学报,2022,38(6) :1582−1591.
WEI J Y,HAN Y T,ZHAI W X,et al. Screening,identification and biocontrol characteristics of antagonistic bacteria against cucumber soil-borne diseases[J]. Chinese Journal of Biological Control,2022,38(6) :1582−1591. (in Chinese)
|
| [18] |
张玉丹,谭琳,任佐华,等. 茶炭疽病拮抗链霉菌的筛选鉴定与拮抗能力测定[J]. 中国生物防治学报,2023,39(3) :646−656.
ZHANG Y D,TAN L,REN Z H,et al. Screening,identification and determination of antagonistic actinomycetes strain against tea anthracnose[J]. Chinese Journal of Biological Control,2023,39(3) :646−656. (in Chinese)
|
| [19] |
宋文欣. 六株拮抗芽胞杆菌对土传病害病原菌的抑制作用及其生物学特性的初步研究[D]. 南宁:广西大学,2020.
SONG W X. Study on the inhibiyion of antagonistic bacillus on the pathogens of soilborne diseases and their biological characteristics[D]. Nanning:Guangxi University,2020. (in Chinese)
|
| [20] |
杨晓云,陈志谊,蒋盼盼,等. 解淀粉芽孢杆菌B1619对番茄的促生作用[J]. 中国生物防治学报,2016,32(3) :349−356.
YANG X Y,CHEN Z Y,JIANG P P,et al. Growth-promotion effect of Bacillus amyloliquefaciens B1619 on tomato plant[J]. Chinese Journal of Biological Control,2016,32(3) :349−356. (in Chinese)
|
| [21] |
宋新娜,梅凤珍,李香风,等. 草莓枯萎病拮抗细菌的筛选及其防治效果[J]. 中国生物防治学报,2022,38(3) :653−661.
SONG X N,MEI F Z,LI X F,et al. Screening and control effect of antagonistic bacteria against strawberry Fusarium wilt[J]. Chinese Journal of Biological Control,2022,38(3) :653−661. (in Chinese)
|
| [22] |
姚锦爱,黄鹏,赖宝春,等. 链霉菌ZZSP-7的鉴定及其对草莓炭疽病的防效[J]. 中国生物防治学报,2021,37(1) :178−183.
YAO J A,HUANG P,LAI B C,et al. Identification and control efficacy of Streptomyces sp. ZZSP-7 against strawberry anthracnose[J]. Chinese Journal of Biological Control,2021,37(1) :178−183. (in Chinese)
|
| [23] |
NTUSHELO K,LEDWABA L K,RAUWANE M E,et al. The mode of action of Bacillus species against Fusarium graminearum,tools for investigation,and future prospects[J]. Toxins,2019,11(10) :606. DOI: 10.3390/toxins11100606
|
| [24] |
黄勋,丰加文,金春林,等. 侧孢短芽胞杆菌JYC 688鉴定及抑菌促生特性研究[J]. 植物保护,2024,50(2) :153−162.
HUANG X,FENG J W,JIN C L,et al. Identification of Brevibacillus laterosporus JYC 688 against potato common scab and its growth promoting characteristics[J]. Plant Protection,2024,50(2) :153−162. (in Chinese)
|
| [25] |
XU W,YANG Q,YANG F,et al. Evaluation and genome analysis of Bacillus subtilis YB-04 as a potential biocontrol agent against Fusarium wilt and growth promotion agent of cucumber[J]. Frontiers in Microbiology,2022,13:885430. DOI: 10.3389/fmicb.2022.885430
|
| [26] |
LI Y,ZHANG X,HE K,et al. Isolation and identification of Bacillus subtilis LY-1 and its antifungal and growth-promoting effects[J]. Plants,2023,12(24) :4158. DOI: 10.3390/plants12244158
|
| [27] |
陶正朋,刘艳霞,李想,等. 烟草青枯病拮抗菌的高通量筛选及其复配菌群防效评估[J]. 中国烟草科学,2024,45(2) :35−45.
TAO Z P,LIU Y X,LI X,et al. High-throughput screening of antagonistic bacteria against tobacco bacterial wilt and biocontrol effects of compound microbial agents[J]. Chinese Tobacco Science,2024,45(2) :35−45. (in Chinese)
|
| [28] |
SUNDARAMOORTHY S,RAGUCHANDER T,RAGUPATHI N,et al. Combinatorial effect of endophytic and plant growth promoting rhizobacteria against wilt disease of Capsicum annum L. caused by Fusarium solani[J]. Biological Control,2012,60(1) :59−67.
|
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