Somatic Incompatibility and Genetic Differences among Strains of <i>Ganoderma sinense</i>

JIANG Xiaohan; YANG Chunyan; WANG Chezhao; LIU Xinrui

doi:10.19303/j.issn.1008-0384.2024.05.006

Volume 39 Issue 5

May 2024

Turn off MathJax

Article Contents

Abstract

References

Fujian Journal of Agricultural Sciences > 2024 > 39(5): 550-556. > DOI: 10.19303/j.issn.1008-0384.2024.05.006

JIANG X H, YANG C Y, WANG C Z, et al. Somatic Incompatibility and Genetic Differences among Strains of Ganoderma sinense [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：550−556. DOI: 10.19303/j.issn.1008-0384.2024.05.006

Citation:

PDF (691 KB)

Somatic Incompatibility and Genetic Differences among Strains of Ganoderma sinense

College of Life Sciences/Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

More Information

Received Date: March 17, 2024
Revised Date: April 09, 2024
Available Online: June 25, 2024

Graphical Abstract

Abstract

Abstract

Objective Somatic incompatibility (SI) and genetic differences among Ganoderma sinense strains were studied.
Method DikaryonG. sinense strains were generated from 7 monokaryon parents by uni- or bi-directional nuclear migration. On a PDA medium, SI between the dikaryon strains was tested, and genetic differentiations examined using ISSR, RAPD, and SRAP molecular markers.
Result Eleven dikaron strains of 5 distinctive genetic types showing a gap, a gap with line, or a wall-like SI were obtained. The combined genetic similarity coefficients of the ISSR, RAPD, and SRAP molecular markers on the strains ranged from 0.29 to 0.97. The UPMGA clustering of the strains corresponded to that of the parents.
Conclusion The SI among the G. sinense strains were mainly associated with the nucleus rather than the cytoplasm. Since the SI types largely paralleled the genetic differences of the dikaron strains, SI determination could be conveniently applied to preliminarily study the genetics of a G. sinense strain.
- antagonism,
- genetic relationship,
- molecular marker

FullText(HTML)

References (21)

References

[1]	黄年来, 林志彬, 陈国良, 等. 中国食药用菌学[M]. 上海: 上海科学技术文献出版社, 2010.
[2]	刘新锐, 王圣铕, 谢宝贵, 等. 紫芝不亲和性因子分析[J]. 菌物学报, 2014, 33(2): 464-468. LIU X R, WANG S Y, XIE B G, et al. Incompatibility factors of Ganoderma sinense[J]. Mycosystema, 2014, 33(2): 464-468. (in Chinese)
[3]	TENG L M, WANG C, CUI B K, et al. Lanostane triterpenoids from mycelia-associated Ganoderma sinense and their anti-inflammatory activity [J]. Phytochemistry, 2023, 215: 113870. DOI: 10.1016/j.phytochem.2023.113870
[4]	GAO S Y, ZHANG P, ZHANG C Y, et al. Meroterpenoids from Ganoderma sinense protect hepatocytes and cardiomyocytes from oxidative stress induced injuries [J]. Fitoterapia, 2018, 131: 73−79. DOI: 10.1016/j.fitote.2018.10.009
[5]	WU N, PENG B, LI T, et al. Rapid simultaneous determination of four ganoderic acids in Ganoderma (chinese Name: Lingzhi) by direct infusion–multiple reaction monitoring cubed [J]. Journal of Analysis and Testing, 2024, 8(1): 52−62. DOI: 10.1007/s41664-023-00271-1
[6]	MEI R Q, ZUO F J, DUAN X Y, et al. Ergosterols from Ganoderma sinense and their anti-inflammatory activities by inhibiting NO production [J]. Phytochemistry Letters, 2019, 32: 177−180. DOI: 10.1016/j.phytol.2019.06.006
[7]	JIANG Y F, CHANG Y J, LIU Y, et al. Overview of Ganoderma sinense polysaccharide-an adjunctive drug used during concurrent Chemo/Radiation therapy for cancer treatment in China [J]. Biomedicine & Pharmacotherapy, 2017, 96: 865−870.
[8]	HAN W, CHEN H J, ZHOU L, et al. Polysaccharides from Ganoderma Sinense - rice bran fermentation products and their anti-tumor activities on non-small-cell lung cancer [J]. BMC Complementary Medicine and Therapies, 2021, 21(1): 169. DOI: 10.1186/s12906-021-03346-7
[9]	LIND M, STENLID J, OLSON A. Genetics and QTL mapping of somatic incompatibility and intraspecific interactions in the basidiomycete Heterobasidion annosum s. l[J]. Fungal Genetics and Biology: FG & B, 2007, 44(12): 1242−1251.
[10]	MARCAIS B, CAËL O, DELATOUR C. Genetics of somatic incompatibility in Collybia fusipes [J]. Mycological Research, 2000, 104(3): 304−310. DOI: 10.1017/S0953756299001069
[11]	唐传红, 张劲松, 陈明杰, 等. 利用拮抗试验和RAPD对灵芝属菌株进行分类研究 [J]. 微生物学通报, 2005, 32(5):72−76. DOI: 10.3969/j.issn.0253-2654.2005.05.015 TANG C H, ZHANG J S, CHEN M J, et al. Study on classification of strains of Ganoderma by anatagonistic effect and rapd [J]. Microbiology, 2005, 32(5): 72−76. (in Chinese) DOI: 10.3969/j.issn.0253-2654.2005.05.015
[12]	李黎. 中国木耳栽培种质资源的遗传多样性研究[D]. 武汉: 华中农业大学, 2011. LI L. Studies on Genetic Diversity of Auricularia Auricula-judae Cultivated Germplasm Resources in China[D]. Wuhan: Huazhong Agricultural University, 2011. (in Chinese)
[13]	张瑞颖. 香菇菌株多相鉴定鉴别技术研究[D]. 北京: 中国农业大学, 2004. ZHANG R Y. Study on Polyphasic Strain-typing Technique of Lentinula Edodes[D]. Beijing: China Agricultural University, 2004. (in Chinese)
[14]	刘靖宇, 宋秀高, 叶夏, 等. 香菇菌株遗传多样性ISSR、RAPD和SRAP综合分析 [J]. 食用菌学报, 2011, 18(3):1−8. DOI: 10.3969/j.issn.1005-9873.2011.03.001 LIU J Y, SONG X G, YE X, et al. Differentiation of Lentinula edodes Strains Using ISSR, RAPD and SRAP markers [J]. Acta Edulis Fungi, 2011, 18(3): 1−8. (in Chinese) DOI: 10.3969/j.issn.1005-9873.2011.03.001
[15]	徐珍, 章炉军, 尚晓冬, 等. 金针菇品种DUS测试性状的分级与评价 [J]. 菌物学报, 2019, 38(5):658−668. XU Z, ZHANG L J, SHANG X D, et al. Gradation and evaluation for Flammulina filiformis DUS testing traits [J]. Mycosystema, 2019, 38(5): 658−668. (in Chinese)
[16]	刘靖宇, 刘新锐, 邓优锦, 等. 双向核迁移在香菇遗传和育种中的应用研究 [J]. 菌物学报, 2011, 30(5):774−781. LIU J Y, LIU X R, DENG Y J, et al. The application of the ‘bidirectional haploid nuclei migration’ in breeding and genetics of Lentinula edodes [J]. Mycosystema, 2011, 30(5): 774−781. (in Chinese)
[17]	CATEN C E. Vegetative incompatibility and cytoplasmic infection in fungi [J]. Journal of General Microbiology, 1972, 72(2): 221−229. DOI: 10.1099/00221287-72-2-221
[18]	WORRALL J J. Somatic incompatibility in basidiomycetes [J]. Mycologia, 1997, 89(1): 24−36. DOI: 10.1080/00275514.1997.12026751
[19]	GIOVANNETTI M, SBRANA C, STRANI P, et al. Genetic diversity of isolates of Glomus mosseae from different geographic areas detected by vegetative compatibility testing and biochemical and molecular analysis [J]. Applied and Environmental Microbiology, 2003, 69(1): 616−624. DOI: 10.1128/AEM.69.1.616-624.2003
[20]	MAY G. Somatic incompatibility and individualism in the coprophilous Basidiomycete, Coprinus cinereus [J]. Transactions of the British Mycological Society, 1988, 91(3): 443−451. DOI: 10.1016/S0007-1536(88)80121-9
[21]	HANSEN E M, STENLID J, JOHANSSON M. Genetic control of somatic incompatibility in the root-rotting basidiomycete Heterobasidion annosum [J]. Mycological Research, 1993, 97(10): 1229−1233. DOI: 10.1016/S0953-7562(09)81290-2

[1]	SHEN Yihui, HE Huibin, CHEN Xiaoyu, YAN Shengnan. Integrated SKNet/Mobilenet V3 Classification of Mango Leaf Diseases and Infestations[J]. Fujian Journal of Agricultural Sciences, 2024, 39(5): 584-592. DOI: 10.19303/j.issn.1008-0384.2024.05.010
[2]	WANG Jingru, ZHANG Wangxiang, DI Changyi, LU Xiaoji. Leaf Color and Pigments of 48 Ornamental Crabapple Germplasms Leaves[J]. Fujian Journal of Agricultural Sciences, 2022, 37(9): 1167-1175. DOI: 10.19303/j.issn.1008-0384.2022.009.007
[3]	HE Na, YANG Yifan, JIANG Hao, PENG Qin, WEI Ziqiu, ZHANG Wangxiang. Correlations among Petal Color, pH, and Epidermal Cell Morphology of Ornamental Crabapple[J]. Fujian Journal of Agricultural Sciences, 2021, 36(9): 1025-1032. DOI: 10.19303/j.issn.1008-0384.2021.09.005
[4]	ZHOU Dan-rong, YE Xin-fu, FANG Zhi-zhen, PAN Shao-lin, JIANG Cui-cui, LIAO Ru-yu, WANG Xiao-an. Research on Relativity of Color with Contents of Anthocyanin,Flavonoids and Arotenoid in Plum[J]. Fujian Journal of Agricultural Sciences, 2015, 30(1): 33-37. DOI: 10.19303/j.issn.1008-0384.2015.01.008
[5]	QIN Zhi-qing. Experimental Starvation on Pomoxis nigromacufatus Larvae and Definition of the Point of no Return[J]. Fujian Journal of Agricultural Sciences, 2014, 29(12): 1167-1173. DOI: 10.19303/j.issn.1008-0384.2014.12.003
[6]	LIN Cheng, WANG Fei, LI Qing-hua, HE Chun-mei, LI Yu, LIU Qi-peng, LIN Li-hong, LIN Xin-jian. Nutrient Content and Enzyme Activity of Cold-waterlogged Soil in Fields with Open Drainage Ditches[J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 1010-1014. DOI: 10.19303/j.issn.1008-0384.2014.10.015
[7]	JIANG Bin, LIN Lin, WU Sheng-hui, ZHANG Shi-zhong. Life Cycle of Cyathocotyle caecumalis sp. nov[J]. Fujian Journal of Agricultural Sciences, 2013, 28(8): 731-735. DOI: 10.19303/j.issn.1008-0384.2013.08.002
[8]	JIN Guang, GUO Rui, LIAO Ru-yu, ZHOU Ping, SHEN Qing-biao, YANG Ling, WU Shao-zhong. Effects of Deastringency with Carbon Dioxide and Alcohol on Fruit Quality and Chloroplast Pigment Content of Persimmon cv. ‘Zaohong’[J]. Fujian Journal of Agricultural Sciences, 2011, 26(5): 842-846.
[9]	LIN Lin, JIANG Bin, WU Sheng-hui, ZHANG Shi-zhong. Preliminary Study on Cyathocotyle caecumalis sp.nov[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 184-188.
[10]	LIN Zhi-min, Song Ya-na, YAO Mei-bin, CHEN Bin, ZHENG Wei-wen. PCR detection and characterization of a phenol-degrading bacteria(Alcaligenes faecalis BC2001)[J]. Fujian Journal of Agricultural Sciences, 2007, 22(1): 50-53.

Cited By

Get Citation

PDF

XML

Article Metrics

Article views (219) PDF downloads (43)

Somatic Incompatibility and Genetic Differences among Strains of Ganoderma sinense

Abstract

References

Related Articles

Catalog

Article Metrics

Related

Somatic Incompatibility and Genetic Differences among Strains of Ganoderma sinense

Abstract

References

Related Articles

Catalog

Article Metrics

Related

Export File

Citation

Format

Content