Somatic incompatibility and genetic difference of <i>Ganoderma sinense</i>

JIANG Xiaohan; YANG Chunyang; WANG Chezhao; LIU Xinrui

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JIANG X H, YANG C Y, WANG C Z, et al. Somatic incompatibility and genetic difference of Ganoderma sinense [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

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JIANG X H, YANG C Y, WANG C Z, et al. Somatic incompatibility and genetic difference of Ganoderma sinense [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

JIANG X H, YANG C Y, WANG C Z, et al. Somatic incompatibility and genetic difference of Ganoderma sinense [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

Citation:

JIANG X H, YANG C Y, WANG C Z, et al. Somatic incompatibility and genetic difference of Ganoderma sinense [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

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Somatic incompatibility and genetic difference of Ganoderma sinense

College of Life Sciences, Fujian Agriculture and Forestry University/Mycological Research Center of Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2024-03-18
Rev Recd Date: 2024-04-10

Available Online: 2024-06-26

Abstract

Abstract

Objective The relation between somatic incompatibility (SI) and genetic difference of Ganoderma sinense was investigated. This will provide the basis for evaluating genetic differences of G.sinense strains by SI test. Method Construction of dikaryon strains using unidirectional or bidirectional nuclear migration test with seven monokaryon of G.sinense as parents. Then SI reaction was tested on the PDA medium, and the genetic differences between them were analyzed by ISSR, RAPD and SRAP molecular markers. Result Eleven dikaron strains of five types with clear genetic relationship were obtained. The SI reactions were result in compatibility and incompatibility. And the incompatibility emerged three types of gap, gap with line and wall-like structure. The combined analysis of ISSR, RAPD and SRAP molecular markers genetic similarity coefficients ranging from 0.29 to 0.97 among the eleven strains.The UPMGA clustering diagram was able to demonstrate the genetic relationship of eleven trains, and these genetic relationships correspond to those of the parental sources. Conclusion The nucleus has a large effect on the SI response of G.sinense, whereas the cytoplasm has a minimal effect on it. The type of SI reaction of G. sinense strains corresponds to their genetic differences. So in the future evaluation of genetic differences in G. sinense germplasm resources, a simple and convenient method of SI could be applied for preliminary analysis.
- antagonism,
- genetic relationship,
- molecular marker

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References(21)

References

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