Role of <i>pAPN</i> and <i>NEU</i>3 in TGEV Infection on Pig

LI Zhaolong; FENG Zhihua; ZHANG Bingchen; FANG Zhou; LIANG Wangwang; CHEN Wenzhi

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LI Z L, FENG Z H, ZHANG B C, et al. Role of pAPN and NEU3 in TGEV Infection on Pig [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：506−514

Citation:

LI Z L, FENG Z H, ZHANG B C, et al. Role of pAPN and NEU3 in TGEV Infection on Pig [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：506−514

LI Z L, FENG Z H, ZHANG B C, et al. Role of pAPN and NEU3 in TGEV Infection on Pig [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：506−514

Citation:

LI Z L, FENG Z H, ZHANG B C, et al. Role of pAPN and NEU3 in TGEV Infection on Pig [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：506−514

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Role of pAPN and NEU3 in TGEV Infection on Pig

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Biomedical Research Center of South China, Fujian Normal University Qishan Campus, Fuzhou, Fujian　350117, China

Received Date: 2020-09-15
Rev Recd Date: 2021-02-08

Available Online: 2021-04-20

Abstract

Abstract

Objective Role of aminopeptidase gene pAPN and sialic acid neuraminidase gene NEU3 in the transmissible gastroenteritis virus (TGEV) infection on pigs was investigated. Methods Being the main receptor of TGEV, pAPN was removed from pAPN and NEU3 in ST cells to verify its supposed key function on the disease. The CRISPR gene editing technique was applied to clip the target gene in ST cells prior to an artificial TGEV infection test. The resulting changes on the infection, virus copy number, cytopathic improvement, and fibronectin were monitored. Results Compared with control, the ST cells free of pAPN and NEU3 significantly attenuated TGEV infection-induced cytopathies and the virus copy number. In addition, at a same TGEV titer the mRNA immune responders induced by the knockdown ST cells were significantly lower than the wild-type counterparts. Conclusion It was confirmed that the removal of pAPN and NEU3 inhibited the TGEV infection in pigs with reduced viral induced cytopathies. Thus, an antiviral therapy and a guideline for breeding resistant pigs could be developed by targeting these two key genes in the ST cells.
- pAPN,
- NEU3,
- TGEV,
- Titer of virus

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References(22)

References

[1]	殷相平, 任晓峰, 柳纪省. 猪传染性胃肠炎病毒致病机制的研究进展 [J]. 世界华人消化杂志, 2013, 21(1):39−43. doi: 10.11569/wcjd.v21.i1.39 YIN X P, REN X F, LIU J X. Progress in understanding pathogenic mechanisms of porcine transmissible gastroenteritis virus [J]. World Chinese Journal of Digestology, 2013, 21(1): 39−43.(in Chinese) doi: 10.11569/wcjd.v21.i1.39
[2]	吴国平, 尹燕博, 吴时友. 猪传染性胃肠炎病毒(TGEV)研究进展 [J]. 中国兽医杂志, 2003, 39(2):29−32. doi: 10.3969/j.issn.0529-6005.2003.02.015 WU G P, YIN Y B, WU S Y. Research progress of transmissible gastroenteritis virus [J]. Chinese Journal of Veterinary Medicine, 2003, 39(2): 29−32.(in Chinese) doi: 10.3969/j.issn.0529-6005.2003.02.015
[3]	柴伟东. 猪传染性胃肠炎基因工程活疫苗的构建与免疫原性测定[D]. 武汉: 华中农业大学, 2010. CHAI W D. Construction and immunogenicity determination of live gene engineering vaccine for infectious gastroenteritis in pigs[D]. Wuhan: Huazhong Agricultural University, 2010. (in Chinese).
[4]	赵珊珊. 猪传染性胃肠炎病毒强弱毒株与猪空肠上皮细胞和猪树突状细胞相互作用的研究[D]. 南京: 南京农业大学, 2014. ZHAO S S. A study on the interaction between porcine jejunal epithelial cells and porcine dendritic cells[D]. Nanjing: Nanjing Agricultural University, 2014. (in Chinese).
[5]	JACOBS L, DE GROOT R, VAN DER ZEIJST B A, et al. The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus (TGEV): Comparison with the sequence of the peplomer protein of feline infectious peritonitis virus (FIPV) [J]. Virus Research, 1987, 8(4): 363−371. doi: 10.1016/0168-1702(87)90008-6
[6]	DELMAS B, GELFI J, L'HARIDON R, et al. Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV [J]. Nature, 1992, 357(6377): 417−420.
[7]	ZHU X, LIU X, WANG X, et al. Contribution of porcine aminopeptidase N to porcine deltacoronavirus iinfcetion [J]. Emerging Microbes infection, 2018, 37(1): 65−70. doi: 10.1038/357417a0
[8]	LI B X, GE ZW, LI YZ, et al. aminopeptidase N is a functional receptor for the PEDV coronavirus [J]. Virology, 2007, 365(1): 166−72.
[9]	REN X, LIU B, YIN J, et al. Phage displayed peptides recognizing porcine aminopeptidase N inhibit transmissible gastroenteritis coronavirus infection in vitro [J]. Virology, 2011, 410(2): 299−306. doi: 10.1016/j.virol.2010.11.014
[10]	DELMAS B, KUT E, GELFI J, et al. Overexpression of TGEV Cell Receptor Impairs the Production of Virus Particles [J]. Advances in Experimental Medicine and Biology, 1995, 380: 379−385.
[11]	LIU B Q, LI G X, REN X F. Initial identification of the domain of TGEV specific receptor APN [J]. Hlongjiang Animal ence and Veterinary Medicine, 2009(6): 21−26.
[12]	SCHWEGMANN-WESSELS C, HERRLER G. Identification of sugar residues involved in the binding of TGEV to porcine brush border membranes [J]. Methods Mol Biol, 2008, 454: 319−329.
[13]	TAKAHASHI K, HOSONO M, SATO I, et al. Sialidase NEU3 contributes neoplastic potential on colon cancer cells as a key modulator of gangliosides by regulating Wnt signaling [J]. International Journal of Cancer, 2015, 137(7): 1560−1573. doi: 10.1002/ijc.29527
[14]	SCHULTZE B, ENJUANES L, CAVANAGH D, et al. N-acetylneuraminic acid plays a critical role for the haemagglutinating activity of avian infectious bronchitis virus and porcine transmissible gastroenteritis virus [J]. Oxygen Transport to Tissue XXXIII, 1993, 342: 305.
[15]	PARK S, SESTAK K, HODGINS D C, et al. Immune response of sows vaccinated with attenuated transmissible gastroenteritis virus (TGEV) and recombinant TGEV spike protein vaccines and protection of their suckling pigs against virulent TGEV challenge exposure [J]. American Journal of Veterinary Research, 1998, 59(8): 1002−1008.
[16]	PENG S Y, LV N, ZHANG Y, et al. Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells [J]. Virus Research, 2007, 128(1/2): 52−57.
[17]	YU T F, LI M, SHAO S L, et al. Genetic Variation Analysis of TGEV Spike Protein Antigenic Sites [J]. Journal of Animal & Veterinary Advances, 2012, 11(3): 361−363.
[18]	GELHAUS S, THAA B, ESCHKE K, et al. Palmitoylation of the Alphacoronavirus TGEV spike protein S is essential for incorporation into virus-like particles but dispensable for S-M interaction [J]. Virology, 2014, 464/465: 397−405. doi: 10.1016/j.virol.2014.07.035
[19]	JORDAN L T, DERBYSHIRE J B. Antiviral action of interferon-alpha against porcine transmissible gastroenteritis virus [J]. Veterinary Microbiology, 1995, 45(1): 59−70. doi: 10.1016/0378-1135(94)00118-G
[20]	THANOS D, MANIATIS T. Virus induction of human IFN beta gene expression requires the assembly of an enhanceosome [J]. Cell, 1995, 83(7): 1091−1100. doi: 10.1016/0092-8674(95)90136-1
[21]	WANG Y Y, LIU W N, PING W, et al. Expression of IFN-γ, IL-6and IL-8 in ST cells infected with TGEV and on the intervention of Lactobacillus acidophilus extracellular polysaccharides [J]. Chinese Journal of Veterinary Medicine, 2015(11): 45−48.
[22]	LI D, LI J W, LI W H, et al. p53 mediated IFN-β signaling to affect viral replication upon TGEV infection [J]. Veterinary Microbiology, 2018(227): 61−68.