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Volume 26 Issue 2
Nov.  2011
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ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.
Citation: ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.
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Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology

  • Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

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  • Received Date: March 01, 2011
  • Revised Date: March 14, 2011
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    • Abstract
  • Conservative fragment of VP5 gene was amplified by RT-PCR and cloned into pMD18-T vector.After sequencing,the positive recombinant plasmid was acquired and used to establish standard and melt curves of the real-time fluorescent quantitative RT-PCR.The standard curve for the threshold cycle and viral genomic copy number ranging from 3.2910～3.29108 were linear.The sensitivity of detection was 33 copies.It was concluded that the real time RT-PCR SYBR GreenⅠ technology was highly specific and repeatable for IBDV diagnostics and quantification.
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    • References (8)
  • ROJS O Z,KRAPEZ U,SLAVEC B,et al.Molecular characterisation of infectious bursal disease viruses isolated in recent acute outbreaks in Slovenia[J].Acta Vet Hung,2008,56 (2):255-264.
    何秀苗,韦平,官丁明,等.2000～2007年广西鸡传染性法氏囊病病毒的分子流行病学[J].病毒学报,2009(6):437-444.
    WANG M Y,HUH L,SUEN S Y,et al.Development of an enzyme-linked immunosorbent assay for detecting infectious bursal disease virus (IBDV) infection based on the VP3 structural protein[J].Vet Microbiol,2008,131 (3-4):229-236.
    王永强,祁小乐,高宏雷,等.鸡传染性法氏囊病病毒实时荧光定量RT-PCR检测方法的建立[J].中国预防兽医学报,2009,31(1):65-68.
    SHI L,LI H,MA G,et al.Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts[J].Virus Genes,2009,39 (1):46-52.
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