Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology

ZHU Chun-hua; LIN Su; CHENG Long-fei; CHEN Hong-mei; CHEN Zhen; LIU Bin-qiong; CAI Guo-zhang; LIN Yu; HUANG Yu

Volume 26 Issue 2

Nov. 2011

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Article Navigation > Fujian Journal of Agricultural Sciences > 2011 > 26(2): 175-179

ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.

Citation:

ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.

Citation:

ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.

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Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology

1.
Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

Received Date: 2011-03-02
Rev Recd Date: 2011-03-15
Publish Date: 2011-04-15

Abstract

Abstract

Conservative fragment of VP5 gene was amplified by RT-PCR and cloned into pMD18-T vector.After sequencing,the positive recombinant plasmid was acquired and used to establish standard and melt curves of the real-time fluorescent quantitative RT-PCR.The standard curve for the threshold cycle and viral genomic copy number ranging from 3.2910～3.29108 were linear.The sensitivity of detection was 33 copies.It was concluded that the real time RT-PCR SYBR GreenⅠ technology was highly specific and repeatable for IBDV diagnostics and quantification.
- infectious bursal disease virus,
- SYBR Green Ⅰ,
- detection

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References(8)

References

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