- 中文核心期刊
- CSCD来源期刊
- 中国科技核心期刊
- CA、CABI、ZR收录期刊
| Citation: |
LIN F Q, CHEN X L, LI D H, et al. Identification and Hexon Gene Variation of Muscovy Duck Adenovirus [J]. Fujian Journal of Agricultural Sciences，2024，39（12）：1346−1353. DOI: 10.19303/j.issn.1008-0384.2024.12.004
|
Characteristics and epidemiology of the muscovy duck white hepatitis in Fujian were studied, and genetic variations of the pathogen capsid protein determined.
Clinical specimens of 9 suspected muscovy duck white hepatitis cases incurred at Fuzhou, Sanming, Putian, and Zhangzhou in the province were collected to test for DAdVs using pathogen detection methods. Isolated pathogens were identified, and their hemagglutination characteristics, pathogenicity, and hexon gene sequences determined.
The disease was found on muscovy ducks mostly in spring and autumn with an incidence rate of 15%–40% and a mortality rate of 5%–15%. The DAdVs were isolated and tested to be lethal to muscovy duck embryos. The infection rate of the virus on ducks ranged 60%–100% with a mortality rate of 20%–40%. The homology of the hexon gene nucleotides among the 9 isolated DAdVs was greater than 99.9%. The capsid proteins had sequences similar to that of CH-GD-12-2014 and were stable proteins without base deletion, insertion or recombination. The 9 isolates had a 99.9%–100% homology with CH-GD-12-2014 from Guangdong, 76.6%–76.7% with DAdV-GR from France, and 64.1%–66.8% with FAdV and other serotypes. They were in the same branch with GD-12-2014 and GR, but not AdV, on the phylogenetic tree.
The strains of the muscovy duck adenovirus circulating in Fujian Province in 2022 belonged to the DAdV Type 2.
| [1] |
ZHANG X H, ZHONG Y J, ZHOU Z H, et al. Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in 2014 [J]. Virology, 2016, 493: 12−21. DOI: 10.1016/j.virol.2016.03.004
|
| [2] |
陈峰, 招丽婵, 覃健萍, 等. 应用宏基因组学鉴定国内第一株鸭2型腺病毒 [J]. 中国预防兽医学报, 2015, 37(12):903−907.
CHEN F, ZHAO L C, QIN J P, et al. Identify a duck adenovirus 2 never reported outside of France by using of metagenomic analysis [J]. Chinese Journal of Preventive Veterinary Medicine, 2015, 37(12): 903−907. (in Chinese)
|
| [3] |
COUNIHAN K L, SKERRATT L F, FRANSON J C, et al. Phylogenetic and pathogenic characterization of novel adenoviruses isolated from long-tailed ducks (Clangula hyemalis) [J]. Virology, 2015, 485: 393−401. DOI: 10.1016/j.virol.2015.07.026
|
| [4] |
HARRACH B,MEGYERI A,PAPP T,et al. A screening of wild bird samples enhances our knowledge about the biodiversity of avian adenoviruses [J]. Veterinary Research Communications, 2023, 47(1): 297−303.
|
| [5] |
陈仕龙, 俞博, 肖世峰, 等. 鸭2型腺病毒感染导致番鸭“肝白化病” 的研究 [J]. 福建农业科技, 2017, 48(8):1−3.
CHEN S L, YU B, XIAO S F, et al. Primary study on duck adenovirus 2 infection resulted in Muscovy ducklings' pale liver disease [J]. Fujian Agricultural Science and Technology, 2017, 48(8): 1−3. (in Chinese)
|
| [6] |
国纪垒, 刁有祥, 薛聪, 等. Ⅰ群禽腺病毒山东株的分离鉴定及hexon基因的克隆与分析 [J]. 中国兽医学报, 2012, 32(12):1773−1777.
GUO J L, DIAO Y X, XUE C, et al. Isolation and identification of inclusion body hepatitis and hexon gene cloning and analysis [J]. Chinese Journal of Veterinary Science, 2012, 32(12): 1773−1777. (in Chinese)
|
| [7] |
林锋强, 程晓霞, 朱小丽, 等. 福建省番鸭小鹅瘟流行毒株病原学研究 [J]. 福建农业学报, 2019, 34(7):796−801.
LIN F Q, CHENG X X, ZHU X L, et al. An etiological study on Muscovy duck-origin goose plague in Fujian [J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 796−801. (in Chinese)
|
| [8] |
MAREK A, KAJÁN G L, KOSIOL C, et al. Complete genome sequences of pigeon adenovirus 1 and duck adenovirus 2 extend the number of species within the genus Aviadenovirus [J]. Virology, 2014, 462: 107−114.
|
| [9] |
江丹丹, 林昶, 黄志坚, 等. 鸭腺病毒B血清1型和血清2型双重PCR检测方法的建立及应用 [J]. 中国预防兽医学报, 2022, 44(8):855−860.
JIANG D D, LIN C, HUANG Z J, et al. Establishment and application of duplex PCR assay for detection of duck adenovirus B serotype 1 and serotype 2 [J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(8): 855−860. (in Chinese)
|
| [10] |
程龙飞, 刘荣昌, 傅光华, 等. 鸭3型腺病毒的分离鉴定及其fiber基因分析 [J]. 中国家禽, 2019, 41(11):47−50.
CHENG L F, LIU R C, FU G H, et al. Isolation and identification of duck adenovirus 3 and sequence analysis of fiber gene [J]. China Poultry, 2019, 41(11): 47−50. (in Chinese)
|
| [11] |
BOUQUET J F, MOREAU Y, MCFERRAN J B, et al. Isolation and characterisation of an adenovirus isolated from Muscovy ducks [J]. Avian Pathology, 1982, 11(2): 301−307. DOI: 10.1080/03079458208436102
|
| [12] |
陈亮, 黄路生, 刘晓文, 等. 鸭2型腺病毒的分离鉴定及hexon基因的序列分析 [J]. 中国兽医科学, 2016, 46(12):1538−1542.
CHEN L, HUANG L S, LIU X W, et al. Isolation and identification of duck adenovirus 2 and sequence analysis of hexon gene [J]. Chinese Veterinary Science, 2016, 46(12): 1538−1542. (in Chinese)
|
| [13] |
NICZYPORUK J S, WOŹNIAKOWSKI G, SAMOREK-SALAMONOWICZ E. Application of cross-priming amplification (CPA) for detection of fowl adenovirus (FAdV) strains [J]. Archives of Virology, 2015, 160(4): 1005−1013. DOI: 10.1007/s00705-015-2355-9
|
| [14] |
张新珩, 蔺文成, 周振海, 等. 新型鸭Ⅲ型腺病毒的分离鉴定及遗传进化分析 [J]. 中国兽医学报, 2017, 37(1):29−32.
ZHANG X H, LIN W C, ZHOU Z H, et al. Isolation, identification and molecular evolution analysis of the new duck adenovirus type Ⅲ virus [J]. Chinese Journal of Veterinary Science, 2017, 37(1): 29−32. (in Chinese)
|
| [15] |
SHI X J, ZHANG X Y, SUN H W, et al. Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China [J]. Poultry Science, 2022, 101(1): 101564. DOI: 10.1016/j.psj.2021.101564
|
| [16] |
CHEN L, YIN L J, PENG P, et al. Isolation and characterization of A novel fowl adenovirus serotype 8a strain from China [J]. Virologica Sinica, 2020, 35(5): 517−527. DOI: 10.1007/s12250-019-00172-7
|
| [17] |
GÜNES A, MAREK A, GRAFL B, et al. Real-time PCR assay for universal detection and quantitation of all five species of fowl adenoviruses (FAdV-A to FAdV-E) [J]. Journal of Virological Methods, 2012, 183(2): 147−153. DOI: 10.1016/j.jviromet.2012.04.005
|
| [1] | WANG Yanjun, ZHANG Fan. Fluorescence Quenching on PEG400-modified ZnS:Cu Quantum Dots and Herbicide Diquat[J]. Fujian Journal of Agricultural Sciences, 2021, 36(11): 1380-1386. DOI: 10.19303/j.issn.1008-0384.2021.11.017 |
| [2] | ZHOU Ruo-lan, SHI Meng-zhu, LI Jian-yu, WANG Yi-shu, FU Jian-wei. Effects of Alternanthera philoxeroides Grown in Different CO2 Environment on the Reproductive Ability of Agasicles hygyophila[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 293-300. DOI: 10.19303/j.issn.1008-0384.2018.03.014 |
| [3] | DING Bo, SHI Meng-zhu, LI Jian-yu, CHEN Jiang, ZHOU Ruo-lan, FU Jian-wei. Responses of Alternanthera philoxeroides and Agasicles hygrophila to Elevated Atmospheric CO2[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 195-200. DOI: 10.19303/j.issn.1008-0384.2017.02.018 |
| [4] | SHI Meng-zhu, FU Jian-wei, LI Jian-yu, WANG Ting, ZHENG Li-zhen, YOU Yong, LIN Tao, WU Gang. Effects of Elevated CO_2 on Consumption and Biochemical Substances of Flea Beetle,Agasicles hygrophila[J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 282-285. DOI: 10.19303/j.issn.1008-0384.2015.03.015 |
| [5] | LAI Long-yu, YAN Zheng-lin, ZHONG You-ping. Density and Composition of the Polyculture of Abalone(H.Discus)and Two Kinds of Alga(U.Lactucaand G.Asiatica)[J]. Fujian Journal of Agricultural Sciences, 2014, 29(9): 832-838. DOI: 10.19303/j.issn.1008-0384.2014.09.003 |
| [6] | WANG Yun. Effect of Abalone Density in Mixed Culture of Abalone and Sea Cucumber[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 633-636. DOI: 10.19303/j.issn.1008-0384.2014.07.005 |
| [7] | LIAN Ling, HE Wei, CAI Qiu-hua, XU Hui-bin, ZHU Yong-sheng, ZHANG Jian-fu, XIE Hua-an. Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 913-918. |
| [8] | LI Hui-ling, LIN Nai-quan. The Influence of Temperature and Humidity on the Population Dynamics of Small Green Leafhopper at Tea Garden[J]. Fujian Journal of Agricultural Sciences, 2012, 27(1): 55-59. |
| [9] | LIN Hai-qing, CHEN Shao-bo. Advance in research on the orientation mechanism of herbivorous insects and natural enemies[J]. Fujian Journal of Agricultural Sciences, 2009, 24(2): 191-196. |
| [10] | Gong Deming, Lin Yanquan, Chen Rukai, Zhang Muqing, Lin Chunhua. Comparisons of Some Photosynthetic Characters and Yields in Various Colony Densities of Sugarcane[J]. Fujian Journal of Agricultural Sciences, 1994, 9(2): 40-45. |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: