• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊
Volume 27 Issue 9
Nov.  2012
Turn off MathJax
Article Contents
Abstract
References
Related Articles
LIAN Ling, HE Wei, CAI Qiu-hua, XU Hui-bin, ZHU Yong-sheng, ZHANG Jian-fu, XIE Hua-an. Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 913-918.
Citation: LIAN Ling, HE Wei, CAI Qiu-hua, XU Hui-bin, ZHU Yong-sheng, ZHANG Jian-fu, XIE Hua-an. Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 913-918.
shu

Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare

  • 1.

    Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China;

  • 2.

    Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China;

  • 3.

    Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology, P.R.China, Fuzhou, Fujian 350003, China;

  • 4.

    National Rice Engineering Laboratory of China, Fuzhou, Fujian 350003, China;

  • 5.

    South China Bases of National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian 350003, China

More Information
  • Received Date: May 31, 2012
  • Revised Date: July 08, 2012
    Graphical Abstract
    • Abstract
  • A cDNA encoding IPA1 was isolated from Nipponbare by RT-PCR, sequencing results showed that the sequence consists of 1 257 bp with a complete CDS and encodes a protein of 418 amino acids. In addition, the prediction results indicated that molecular weight of target protein was 42.51 kD and theoretical isoelectric point was 9.37. The plant expression vector was constructed by ligating the cDNA fragment into expression vector pHI, then the recombinant plasmid pHI-IPA1 was confirmed by restriction enzyme digestion analysis with SmaI、SacI, and further verified by DNA sequencing. Vector plasmid was transformed into Agrobacterium tumefaciens, which will lay foundation for rice genetic transformation.
    • FullText(HTML)
    • References (2)
  • 谢华安.中国特别是福建的超级稻研究进展[J].中国稻米,2004,(2):7-9.
    周开达,马玉清,刘太清,等.杂交水稻亚种间重穗型组合的选育——杂交水稻超高产育种的理论与实践[J].四川农业大学学报,1995,13(4): 403- 407.
    • Related Articles

  • Related Articles

    [1]WU Xianfeng, LIU Yuan, WANG Yinggang, ZHANG Fu, LI Wenyang. Relationship between InDel Polymorphism of FN1 Gene and Growth Traits of Goats[J]. Fujian Journal of Agricultural Sciences, 2023, 38(1): 1-6. DOI: 10.19303/j.issn.1008-0384.2023.01.001
    [2]XU Yi, LI Yujia, WEI Qing, WANG Anbang, WANG Xiaoyi, SONG Shun, LI Jingyang. Constructions of Banana MaAQP1 Bait Vector and Drought-resistance cDNA Library[J]. Fujian Journal of Agricultural Sciences, 2020, 35(10): 1078-1085. DOI: 10.19303/j.issn.1008-0384.2020.10.005
    [3]CHEN Si-rong, ZHAO Feng, WANG Shu-yan, JIN Shan, ZHOU Peng, WEI Sai-ming, YE Nai-xing. Amino Acids in White Peony Teas of Different Grades[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 965-973. DOI: 10.19303/j.issn.1008-0384.2019.08.015
    [4]CHEN Hua, LI Su-yi, ZHANG Li-juan, KE Ling, CHEN Xu, LIN Chen-tao. Functions of CpG-ODN 2006 on Resistance of Zebra Fish against Vibrio vulnificus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(1): 1-6. DOI: 10.19303/j.issn.1008-0384.2018.01.001
    [5]ZHANG Dan-dan, YE Xiao-hui, ZHAO Feng, WANG Dan-hong, WEI Sai-ming, ZHENG De-yong, YE Nai-xing. Flavor and Amino Acids of Brewed White Teas[J]. Fujian Journal of Agricultural Sciences, 2016, 31(5): 515-520. DOI: 10.19303/j.issn.1008-0384.2016.05.014
    [6]JIAO Yun, SHU Qiao-yun, LIU Zhu-qin. Single Nucleotide Polymorphism (SNP) Analysis of the Endopolygalacturonase Gene in Chinese Bayberry (Myrica rubra)[J]. Fujian Journal of Agricultural Sciences, 2016, 31(5): 465-470. DOI: 10.19303/j.issn.1008-0384.2016.05.005
    [7]HUANG Huan, ZHAO Zhan-heng, WANG Yu-jiao, YOU Qing-han, YI Mei-gui, LIANG Yu-xiang. Study on the Content Change of Caffeine,Tea Polyphenols and Free Amino Acids in the Tie-guanyin Oolong Tea Machining Process[J]. Fujian Journal of Agricultural Sciences, 2014, 29(3): 282-285. DOI: 10.19303/j.issn.1008-0384.2014.03.017
    [8]ZHANG Ze-huang, DENG Chao-jun, ZHOU Dan-rong, CHEN Xiu-ping. Free Amino Acids in Loquat Flowers[J]. Fujian Journal of Agricultural Sciences, 2011, 26(5): 753-757.
    [9]LUO Qin, CHEN Ren-bi, SONG Yong-kang. Determination method of oligopeptides and free amino acids in fish meal[J]. Fujian Journal of Agricultural Sciences, 2005, 20(4): 285-288.
    [10]Lu Tong, Cai Qinghua, Yu Hua, Chen Xin, Song Xiaochuan, Zhang Lianshun. Research about Relationship Between The Isozymes of Esterase Peroxidase and the Resistance to Pseudomonas solanacearum in Sweet Potato Varieties[J]. Fujian Journal of Agricultural Sciences, 1992, 7(1): 72-77.

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (2788) PDF downloads (609) Cited by()
    Related

    Copyright © 2018 Fujian Journal of Agricultural Sciences, All Rights Reserved. 闽ICP备19004781号-1 网安备：35010046024-23001

    Address: 247 Wusi Road, Fuzhou City China Pos: 350003 Tel: 0591-87869455 E-mail: fjnyxb@163.com

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return