Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines

LI Shujun; LIU Yajuan; ZHANG Yang; PU Rumin; YUAN Liang; LIAO Changjian

doi:10.19303/j.issn.1008-0384.2024.03.002

Volume 39 Issue 3

Mar. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(3): 251-258

LI S J, LIU Y J, ZHANG Y, et al. Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines [J]. Fujian Journal of Agricultural Sciences，2024，39（3）：251−258 doi: 10.19303/j.issn.1008-0384.2024.03.002

Citation:

LI S J, LIU Y J, ZHANG Y, et al. Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines [J]. Fujian Journal of Agricultural Sciences，2024，39（3）：251−258 doi: 10.19303/j.issn.1008-0384.2024.03.002

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Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines

doi: 10.19303/j.issn.1008-0384.2024.03.002

1.
Maize Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing　401329, China
2.
Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan　611130, China
3.
Institute of Crop Research, Fujian Academy of Agricultural Sciences (Fujian Germplasm Resources Center)/Fujian Province Characteristic Dry Crop Variety Breeding Engineering Technology Research Center, Fuzhou, Fujian　350013, China

Received Date: 2023-11-22
Rev Recd Date: 2024-02-05

Available Online: 2024-05-08

Publish Date: 2024-03-28

Abstract

Abstract

Objective Sweet corn inbred lines low in accumulating Pb and Cd were identified, and a molecular detection method for the pollutants examined. Method Ten sweet corn inbred lines were tested on Pb and Cd accumulation. In a pot experiment, the heavy metals in roots, stems/leaves, and kernels of the plants grown on soil with spiked Pb and/or Cd were measured over time. Applicability of a molecular detection method on Pb and Cd at the InDel locus of ZmHMA2 in corn plant was scrutinized. Result The accumulation of Pb and Cd in organs of a corn plant was roots＞stems/leaves＞kernels. It did not differ significantly whether the metal elements were presented individually or simultaneously in the soil. Two inbred lines were identified to retain in the kernels less on Pb, 3 on Cd, and only Mintian X901 on both Pb and Cd. The targeted molecular detection found InDel 2307 locus in 4 of the specimens, the average content of Cd which contained were 1.801 mg·kg⁻¹ lower in the roots, 0.64 mg·kg⁻¹ lower in the stems/leaves, and 0.131 mg·kg⁻¹ lower in the kernels compared to the average of the other varieties. Conclusion The InDel 2307 locus as a marker displayed a specificity in differentiating Cd content in corn plants grown on soils contaminated with different levels of the heavy metals. The molecular detection result and the Pb and Cd contents on the 10 sweet corn inbred lines indicated that Mintian X901 was the cultivar least prone to accumulate the pollutants.
- Heavy metal,
- Pb pollution,
- Cd pollution,
- sweet corn,
- accumulation,
- marker detection

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References(32)

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