Effect of 2, 4-Brassinosteroid on Growth of <i>Hordeum vulgare</i> Seedlings under Cd-stress

LI Xiao-ke; WU Yu-zhen; ZHANG Yi-xian

doi:10.19303/j.issn.1008-0384.2018.12.004

Volume 33 Issue 12

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(12): 1251-1256

LI Xiao-ke, WU Yu-zhen, ZHANG Yi-xian. Effect of 2, 4-Brassinosteroid on Growth of Hordeum vulgare Seedlings under Cd-stress[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1251-1256. doi: 10.19303/j.issn.1008-0384.2018.12.004

Citation:

LI Xiao-ke, WU Yu-zhen, ZHANG Yi-xian. Effect of 2, 4-Brassinosteroid on Growth of Hordeum vulgare Seedlings under Cd-stress[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1251-1256. doi: 10.19303/j.issn.1008-0384.2018.12.004

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Effect of 2, 4-Brassinosteroid on Growth of Hordeum vulgare Seedlings under Cd-stress

doi: 10.19303/j.issn.1008-0384.2018.12.004

1.
School of Biology Science and Technology, Jinzhong College, Jinzhong, Shanxi 030619, China
2.
School of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China

Received Date: 2018-09-06
Rev Recd Date: 2018-10-28
Publish Date: 2018-12-28

Abstract

Abstract

Effect of 2, 4-brassinosteroid on the growth of barley (Hordeum vulgare) seedlings under Cd-stress were studied by an indoor culture experiment. In comparison with control, the treatment of Cd at 20 mg·L^-1 inhibited the seedling growth and damaged the leaf physiology manifesting in the decreased root length and plant height, reduced contents on biomass, chlorophyll, soluble sugars and soluble protein, declined activities of POD and CAT, increased relative electrical conductivity, heightened contents of MDA, O₂^- and proline, and elevated activity of SOD. After spraying 2, 4-brassinosteroid at 0.2 mg·L^-1, the Cd-stressed seedlings significantly increased on the root length, plant height, biomass and chlorophyll. The treatment promoted the photosynthesis and seedling growth, reduced the leaf relative conductivity, MDA and O₂^-, and facilitated an osmotic adjustment with the substances (e.g., soluble sugars, proline and soluble protein), and enhanced the antioxidant enzymatic activities on SOD, POD and CAT. It appeared that the plant hormone could alleviate the ill-effects of Cd-stress on and enrich the adaptability in the battle against the adversity for the barley seedlings.
- Cd-stress,
- Hordeum vulgare,
- brassinosteroid,
- seedling growth

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References(39)

References

[1]	郑洁, 王磊.油菜素内酯在植物生长发育中的作用机制研究进展[J].中国农业科技导报, 2014, 16(1):52-58. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgnykjdb201401009
[2]	CHOUDHARY S P, YU J Q, YAMAGUCHI-SHINOZAKI K, et al.Benefits of brassinosteroid crosstalk[J].Cell, 2012, 17(10):594-605. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ff2e8209407fcdfeb0a8c3748ca2b962
[3]	PRITI K.Brassinosteroid-mediated stress responses[J].Plant Growth Regul, 2003, 22(4):289-292. doi: 10.1007/s00344-003-0058-z
[4]	ZHANG C, BAI M Y, CHONG K.Brassinosteroid-mediated regulation of agronomic traits in rice[J].Plant Cell Rep, 2014, 33(5):683-696. doi: 10.1007/s00299-014-1578-7
[5]	何俊瑜, 任艳芳, 王阳阳, 等.不同耐性水稻幼苗根系对镉胁迫的形态及生理响应[J].生态学报, 2011, 31(2):522-528. http://d.old.wanfangdata.com.cn/Periodical/stxb201102026
[6]	HAYAT S, ALI B, HASAN S A, et al. Brassinosteroids enhanced the level of antioxidants under cadmium stress in Brassica juncea[J].Environmental and Experimental Botany, 2007, 60(1):33-41. doi: 10.1016/j.envexpbot.2006.06.002
[7]	郑爱珍.镉胁迫对芥蓝根系质膜过氧化及ATPase活性的影响[J].生态学报, 2012, 32(2):483-488. http://d.old.wanfangdata.com.cn/Periodical/stxb201202018
[8]	HASAN S A, HAYAT S, ALI B, et al.28-Homobrassinolide protects chickpea from cadmium toxicity by stimulating antioxidants[J].Environmental Pollution, 2008,151(1):60-66. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=2baa0ccb697e9766bb9045f51a72c1d2
[9]	曾翔, 张玉烛, 王凯荣, 等.镉对水稻种子萌发的影响[J].应用生态学报, 2007, 18(7):1665-1668. doi: 10.3321/j.issn:1001-9332.2007.07.041
[10]	顾自奋, 黄志仁, 许如根, 等.近10年世界大麦生产概况[J].大麦科学, 2001, 18(1):1-4. doi: 10.3969/j.issn.1673-6486.2001.01.001
[11]	殷欣, 张海玲, 吴杨, 等.24-表油菜素内酯对镉胁迫下大豆苗期生理特性的影响[J].核农学报, 2016, 30(2):364-371. http://d.old.wanfangdata.com.cn/Periodical/hnxb201602021
[12]	高会玲, 刘金隆, 郑青松, 等.外源油菜素内酯对镉胁迫下菊芋幼苗光合作用及镉富集的调控效应[J].生态学报, 2013, 33(6):1935-1943. http://d.old.wanfangdata.com.cn/Periodical/stxb201306028
[13]	袁博, 金晓霞, 肖玮, 等.外源表油菜素内酯(2, 4-EBL)对镉(Cd)胁迫下番茄幼苗的缓解效应[J].分子植物育种, 2018, 16(9):2955-2965. http://d.old.wanfangdata.com.cn/Thesis/Y3402255
[14]	张义贤, 李晓科.镉、铅及其复合污染对大麦幼苗部分生理指标的影响[J].植物研究, 2008, 28(1):43-46. http://d.old.wanfangdata.com.cn/Periodical/zwyj200801010
[15]	肖志华, 张义贤, 张喜文, 等.外源铅、铜胁迫对不同基因型谷子幼苗生理生态特性的影响[J].生态学报, 2012, 32(3):889-897. http://d.old.wanfangdata.com.cn/Periodical/stxb201203025
[16]	李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000:184-185,258-261.
[17]	XIE Z, DUAN L, TIAN X, et al.Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity[J].Journal of Plant Physiology, 2008,165(4):375-384. doi: 10.1016/j.jplph.2007.06.001
[18]	燕傲蕾, 吴亭亭, 王友保, 等.三种观赏植物对重金属镉的耐性与积累特性[J].生态学报, 2010, 30(9):2491-2498. http://d.old.wanfangdata.com.cn/Periodical/stxb201009030
[19]	ARIFA K, FAHEEM A.Effect of exogenous application of 2, 4-epibrassinolideon growth, protein contents, and antioxidant enzyme activities of in vitro-grown Solanum tuberosum L.under salt stress, In Vitro Cellular & Devlopmental Biology-Plant[J].Dev Biol Plant, 2016, 52(1):1-11.
[20]	王晓娟, 王文斌, 杨龙吴, 等.重金属镉(Cd)在植物体内的转运途径及其调控机制[J].生态学报, 2015, 35(23):7921-7929. http://d.old.wanfangdata.com.cn/Periodical/stxb201523035
[21]	吴杨, 高慧纯, 张必弦, 等.2, 4-表油菜素内酯对盐碱胁迫下大豆生育、生理及细胞超微结构的影响[J].中国农业科学, 2017, 50(5):811-821. http://www.cnki.com.cn/Article/CJFDTotal-ZNYK201705004.htm
[22]	HAUBRICK L L, ASSMANN S M. Brassinosteroids and plant function:some clues, more puzzles[J].Plant Cell and Environment, 2006, 29(3):446-457. doi: 10.1111/pce.2006.29.issue-3
[23]	王爱君, 王志敏, 汤青林, 等.超干处理对种子生物膜及种子活力的影响[J].南方农业:园林花卉版, 2011, 5(4):52-54. http://d.old.wanfangdata.com.cn/Periodical/nfny-lshyb201102016
[24]	乔琳, 李杰, 胡春红, 等.外施表油菜素内酯缓解玉米幼苗铅毒害机制研究[J].核农学报, 2014, 28(11):2126-2131. doi: 10.11869/j.issn.100-8551.2014.11.2126
[25]	SANTOS L R, BATISA B L, LOBATO A K S.Brassinos-teroids mitigate cadmium toxicity in cowpea plants[J].Photosynthetica, 2017, 55(4):1-15.
[26]	SYED A H B, WANG R F, IMRUL M A, et al.Genotype-dependent effect of exogenous 24-epibrassinolide on chromium-induced changes in ultrastructure and physicochemical traits in tobacco seedlings[J]. Environ Sci Pollut Res, 2016, 23(18):1-10. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=3bd9f43c55ed401f44905c8f1dd2f0c0
[27]	靳开川, 何金环.油菜素内酯在植物抗逆中的作用及信号传导机制综述[J].江苏农业科学, 2017, 45(14):4-7. http://d.old.wanfangdata.com.cn/Periodical/jsnykx201714002
[28]	HASAN S A, HAYAT S, AHMAD A. Brassinosteroids protect photosynthetic machinery against the cadmium induced oxidative stress in two tomato cultivars[J].Chemosphere, 2011, 84(10):1446-1451. doi: 10.1016/j.chemosphere.2011.04.047
[29]	尹博, 王秀峰, 姜春辉, 等.外源油菜素内酯对番茄铜胁迫的缓解效应[J].植物营养与肥料学报, 2012, 18(1):162-168. http://d.old.wanfangdata.com.cn/Thesis/Y2116982
[30]	XIA X J, HUANG L F, ZHOU Y H. Brassinosteroids promote photosynthesisand growth by enhancing activation of Rubisco and expression of photosynthetic genes in Cucumis sativus L.[J].Planta, 2009,230:1185-1196. doi: 10.1007/s00425-009-1016-1
[31]	DING J, SHI K, ZHOU Y H.Effects of root and foliar applications of 2, 4-epibrassinolide on fusarium wilt and antioxidant metabolism in cucumber roots[J].Hort Science, 2009, 44:1340-1345. doi: 10.1007%2Fs10658-008-9401-3
[32]	张义贤, 付亚萍, 肖志华, 等.铅胁迫对不同基因型谷子幼苗生理特性及基因组DNA多态性的影响[J].农业环境科学学报, 2013, 32(3):478-484. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=nyhjbh201303011
[33]	ANURADHA S, RAO S.The effect of brassinosteroids on radish seedlings growing under Cadmium stress[J].Plant Soil and Environment, 2007, 53(11):465-472.
[34]	钟杨, 陈洪国.芸苔素内酯水剂对辣椒幼苗水分胁迫生理的影响[J].北方园艺, 2011(2):42-44. http://d.old.wanfangdata.com.cn/Periodical/bfyany201102014
[35]	魏湜, 吉彪, 李赞, 等.盐胁迫下油菜素内酯对玉米幼苗生理特性的影响[J].东北农业大学学报, 2018, 49(5):9-16. doi: 10.3969/j.issn.1005-9369.2018.05.002
[36]	郑爱珍.镉胁迫对芥蓝根系质膜过氧化及ATPase活性的影响[J].生态学报, 2012, 32(2):483-488. http://d.old.wanfangdata.com.cn/Periodical/stxb201202018
[37]	李涛涛, 高永峰, 马瑄, 等.外源油菜素内酯对三种杨树在干旱、盐和铜胁迫下光合生理的影响[J].基因组学与应用生物学, 2016, 35(1):218-226. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jyzxyyyswx201601034
[38]	FARIDUDDIN Q, MIR B A, YUSUF M, et al.2, 4-epibrassinolide and/or putrescine trigger physiological and biochemical responses for the salt stress mitigation in Cucumis sativus L[J].Photosynthetica, 2014, 52(3):464-474. doi: 10.1007/s11099-014-0052-7
[39]	BAJGUZ A, HAYAT S.Effects of brassinosteroids on the plant responses to environmental stresse[J].Plant Physiology and Biochemistry, 2009, 47(1):1-8.