Optimization of Technical Conditions for Tissue Culture and Rapid Propagation of Chinese Yam

With'Ming Huai yam 2' as the experimental material, we optimized the technological conditions of the rapid propagation of yam. The results showed that in vitro culture, the best explant was segment with bud in middle part of new shoot. The best disinfection method was with 70% alcohol immersion 10 s, and 0.1% HgCl₂ for 10 min, the pollution rate of which was 15.0%, and the mortality rate was 6.7%. The primary culture medium was:MS+6-BA 1.0 mg+NAA 0.05 mg·L^-1+PVP 100 mg·L^-1+VC 500 mg·L^-1 +AC 0.5 g·L^-1+Sug 30 g·L^-1. After two weeks, it was transferred to the solid medium with the same formula. The browning rate was 36.7% and the induction rate was 81.7%. The subculture multiplication medium was:MS+6-BA 2.0 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.05 mg·L^-1+PP₃₃₃ 0.05 mg·L^-1+Agar 4.0 g·L^-1+Sug 30 g·L^-1. The proliferation coefficient of 30 d was 2.97. The rooting medium was:1/2MS+NAA 0.3 mg·L^-1+IBA 0.2 mg·L^-1+AC 1.0 g·L^-1+Agar 4.5 g·L^-1+Sug 20 g·L^-1. The rooting rate was 91.3%. After two weeks of acclimation in the greenhouse, the plants were soaked with carbendazim and cooled to dry, then transplanted to peat soil tray. The transplanting survival rate was more than 70%.