Efficient Germination System in Asymbiotic Culture of <i>Bletilla Striata</i> (Thunb.)Reichb. F.

CHEN Jing-ying; WAN Xue-feng; LIU Bao-cai; ZHAO Yun-qing; HUANG Ying-zhen; ZHANG Wu-jun

doi:10.19303/j.issn.1008-0384.2018.02.005

Volume 33 Issue 2

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(2): 131-135

CHEN Jing-ying, WAN Xue-feng, LIU Bao-cai, ZHAO Yun-qing, HUANG Ying-zhen, ZHANG Wu-jun. Efficient Germination System in Asymbiotic Culture of Bletilla Striata (Thunb.)Reichb. F.[J]. Fujian Journal of Agricultural Sciences, 2018, 33(2): 131-135. doi: 10.19303/j.issn.1008-0384.2018.02.005

Citation:

CHEN Jing-ying, WAN Xue-feng, LIU Bao-cai, ZHAO Yun-qing, HUANG Ying-zhen, ZHANG Wu-jun. Efficient Germination System in Asymbiotic Culture of Bletilla Striata (Thunb.)Reichb. F.[J]. Fujian Journal of Agricultural Sciences, 2018, 33(2): 131-135. doi: 10.19303/j.issn.1008-0384.2018.02.005

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Efficient Germination System in Asymbiotic Culture of Bletilla Striata (Thunb.)Reichb. F.

doi: 10.19303/j.issn.1008-0384.2018.02.005

CHEN Jing-ying^{1, 2
,},
WAN Xue-feng^{2, 3},
LIU Bao-cai^{1, 2},
ZHAO Yun-qing^{1, 2},
HUANG Ying-zhen^{1, 2},
ZHANG Wu-jun^{1, 2}

1.
Institute of Agricultural Bioresource, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
2.
Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
3.
Zhangzhou Institute of Agricultural sciences, Zhangzhou, Fujian 363005, China

Received Date: 2017-07-07
Rev Recd Date: 2017-10-18
Publish Date: 2018-02-01

Abstract

Abstract

The mature capsule of Bletilla striata was used as material for asymbiotic germination in this experiment. With MS as basal medium, effects of different sowing ways, seedling rates, storage ways, light conditions and additives on the seed germination of Bletilla striata were studied. The results showed that the best germination conditions for asymbiotic culture of Bletilla striata is with seedling rate 5×10³ grains·mL^-1 in suspended liquid, and cultured in dark conditions for the first 14 days, the germination rate of which reached 93%. Adding 10% mashed potato or coconut juice could increase the germination rate, and promote the growth of protocorm. The seeds of Bletilla striata stored in plastics bags at 4℃ was beneficial for germination, the germination rate for which could reach 50% after 60 days.
- Bletilla striata,
- seed,
- asymbiotic culture,
- germination

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References(31)

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