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2012 Vol. 27, No. 1

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Preliminary Genotype Analysis of Broad-spectrum Rice Blast Resistance Variety Yunyin and its Near-isogenic Lines Construction
ZHU Yong-sheng, CAI Qiu-hua, XUE Wei-min, XIE Hong-guang, WU Fang-xi, ZHANG Jian-fu, XIE Hua-an
2012, 27(1): 1-6.
Abstract:
The phenotype of broad-spectrum rice blast resistance variety Yunyin was analyzed and identified by inoculated with rice blast fungi RB01-RB22 of known avirulence genes as pathogen in vitro. The results showed that Yunyin was resistant to all rice blast fungi except RB17 and RB20.Analyzing the widely used 24 resistance genes in production at present, the results showed that Yunyin may contain new blast resistance genes besides these genes, and there were able to be applied for rice breeding and production. In addition, in this study, we constructed near-isogenic lines with LTH as genetic background and contained blast-resistant gene to blast isolate SiChuan 43 by successive backcrossing combining marker-assisted selection with artificial inoculation.These works would be beneficial to the genetics study and application of this blast-resistance gene.
Real-time PCR Analysis of Genes Induced with the Magnaporthe oryzae from Japonica Rice Yunyin
WANG Ying-heng, ZHU Yong-sheng, CAI Qiu-hua, LIAO Chang-jian, XIE Hong-guang, WU Fang-xi, ZHANG Jian-fu, XIE Hua-an
2012, 27(1): 7-12.
Abstract:
In early stage, we analysed the gene expression profile of broad-spectrum rice blast resistance japonica variety Yunyin induced by Mangnaporthe oryzae, and constructed the gene co-expression network based on the dynamic samples. Ten important genes based on this network were chosen in this study, and the expression pattern of 10 genes for japonica rice Yunyin inoculated with Mangnaporthe grisea was analyzed. The highest expression stages of the 10 genes were different, of which 8 genes reached the highest expression stage at 36 hours, and 3 genes encoding transcription factors reached the highest expression stage at 24 hours. Meanwhile, some of the 10 genes expression were up-regulated at first, and then inhibited. Others were inhibited at first, then increased, and declined at last. This work will help us further to define the role of japonica rice Yunyin in disease resistance.
Studies on Agronomic Measures for High Yield Virus-free Sweet Potato "Longyan 7-3"
LIN Jin-hu
2012, 27(1): 13-17.
Abstract:
To study the effects of NPK fertilizer and planting density on potato yield,virus-free sweet potato "Longyan 7-3" was cultured with 4 factors ×5 levels quadratic general rotational design. A high yield mathematical model was built by computer simulation to obtain the optimum agronomic measures. The results showed that: (1) the optimal combination of 4 factors for yield over 45.0 t·hm-2 was: applying urea between 163.05-191.55 kg·hm-2, crude calcium 546.6-653.4 kg·hm-2, potassium sulphate 510.3-614.55 kg·hm-2, and planting density at 5.45×104-5.80×104 plants per ha; (2) the effect of 4 factors on potato yield was in order of K>planting density>N>P fertilizer; (3) the interaction effects on the yield were observed among the factors; (4) increase of K and N fertilizers were conducive to the yield enhancement of Longyan 7-3; and (5) the suitable planting density resulted the high proportion of large and medium size tuber.
Fertilizer Efficiency and Optimum Recommendation of NPK Fertilizer for Mid-season Rice in the Hinterland of Jianghan Plain
LIU Xin-wei, GONG De-ping, LOU Xi-feng, HAN Ling-jun, YANG De-hua, ZHAO Zhu-qing
2012, 27(1): 18-23.
Abstract:
A "3414" fertilizer experiment was carried out in Jiangbei farm of five fields to determine the yield and economic effect of nitrogen, phosphorus and potassium fertilization on mid-season rice. The result showed that the effect of fertilization on mid-season rice yield increase is obvious: the effect of N fertilization on mid-season rice yield and economic benefit was largest,the next was P, and K fertilization was the smallest. The highest in yield of N,P and K were 695.1-2 414.7 kg穐m-2, and which were 8.6%-37.8% higher than contro1.The net income of N, P and K were 1 011.2-3 563.5 Yuan穐m-2, respectively. According to fertilizer effect, N, P2O5 and K2O application rate to get the highest profit were 180-200 kg穐m-2, P2O5 55-60 kg穐m-2, and K2O 50-60 kg穐m-2, respectively.
Differences in the Physicochemical Characteristics and Pathogenicity Between European Type PRRSV and North American Type PRRSV
HUANG Mei-qing, ZHENG min, CHEN Shi-long, CHE Yong-liang, JIANG Bin, WANG Long-bai, WEI Hong, ZHOU Lun-jiang, ZHUANG Xiang-sheng, CHEN Shao-ying
2012, 27(1): 24-26.
Abstract:
The Physicochemical Characteristics and Pathogenicity of five PRRSV strains including two European types PRRSV and three North American types PRRSV were studied, which were isolated from pig farms in Fujian province. The results showed that all the five PRRSV strains could induce regular cytopathogenic effects (CPE) on Marc-145 cells, but CPE was not appeared on PK-15,Vero,CEF or MDEF cells after three times of blind passages. The five PRRSV strains showed the same sensitive to acid, pH value 3, chloroform and trypsin, but different to the temperature. Compared with the North American types PRRSV, the European types PRRSV were more resistant to pH value 9.It was confirmed that the European type PRRSV-FJ0602 has a low pathogenicity to pigs but the North American type PRRSV-FJ0604 has a high pathogenicity to pigs according to the animal experiment.
Influence of Astragalus Polysaccharides on Leukocyte Phagocytosis Activity and Immune Organs Indexes of Tilapia
LIN Xuan, ZHANG Wei-ni, WANG Shou-kun, HUANG Wen-yan, CHEN Jia-ming, ZHAO Jin
2012, 27(1): 27-31.
Abstract:
One hundred and twenty tilapia were randomly divided into 5 groups in this experiment.Four test groups were fed with basic diet with different levels of astragalus polysaccharides (100, 500, 1 000, 1 500 mg穔g-1), while control group was only fed with basic diet for 40 days.The leukocyte phagocytosis activity and the weight of immune organs of tilapia were detected respectively every ten days.The result showed that group 500 mg穔g-1,1 000 mg穔g-1 and 1 500 mg穔g-1 were significantly different with the control group on phagocytosis activity and the growth of immune organs (P-1 did not have significantly different with the control group (P>0.05).It indicated that astragalus polysaccharides could enhance phagocytosis activity and the growth and maturity of immune organs.
Studies on Meiotic Behavior of Pollen Mother Cells of bitter gourd (Momordica charantia L.)
CHEN Long-zheng, ZHANG Hui, ZHANG Yong-ji, XU Hai, SONG Bo, YUAN Xi-han
2012, 27(1): 32-36.
Abstract:
Meiotic behaviors of pollen mother cells (PMCs) of bitter gourd (Momordica charantia L.) were characterized. It was found that the pollen mother cell of bitter gourd revealed a simultaneous cytokinesis with unsynchronized phenomenon in meiotic division. Nucleolus disappeared gradually from prophase I to metaphase I, reappeared at prophaseⅡand then disappeared. Chromosome became more and more identifiable from prophase I to metaphase I, and illegible at telophase I with unwinding. Moreover, multi nucleoli was investigated in prophase I and prophase Ⅱ. Frequent polyads were observed in this study, which was likely related to the weather adversity and unsynchronized phenomenon in meiotic division.
Genetic Relationship among Banana (Musa spp.) Germplasms Revealed by ISSR Analysis
YAO Jin-ai, CAI Hong-jiao, SHI Niu-niu, GAN Lin, YANG Xiu-juan, CHEN Fu-ru
2012, 27(1): 37-42.
Abstract:
Genetic diversity and relationship among 35 banana germplasms involving 3 genotypes (i.e., AAA, AA and ABB) were studied by using the Inter-Simple Sequence Repeat (ISSR) method.As a result, 6 ISSR primers were selected, 38 bands amplified between 200 bp and 2,000 bp, 27 bands detected for polymorphic DNA, and the percentage of polymorphic DNA 71.05% of polymorphic DNA were found.The genetic distances among the banana cultivars were between 4.30% and 33.33%.A cluster analysis was performed applying the unweighted pair group method on arithmetic averages.The banana germplasms were grouped into 6 clusters based on the genetic distance of 25%.Except for Gongjiao (AA) and Jinfen1 (ABB), which were grouped into Cluster III and IV, respectively, the remaining 33 germplasms were classified into 4 clusters, in which Cluster I contained 22 germplasms from different localities and genetypes (AAA and ABB).All germplasms with genotypes AAA and ABB could not be divided into 2 clusters.There appeared no close genetic relationship among some banana germplasms with same genotypes.The germplasms could be discriminated by the 6 selected primers, and ISSR was considered as an effective method for studying genetic relationship among different banana germplasms resources.
Resistance Evaluation of Rice New Varieties Against Rice False Smut and Rice Neck Blast in Fujian Province
RUAN Hong-chun, SHI Niu-niu, DU Yi-xin, GAN Lin, WANG Shu-ting, ZENG Hui-fang, YANG Xiu-juan, CHEN Fu-ru
2012, 27(1): 43-47.
Abstract:
The resistance evaluation of 15 rice new varieties to rice false smut and rice neck blast disease was tested in Fujian Province by using methods of artificial injected inoculation in greenhouse and natural inducing in field, respectively. The evaluation results of rice resistance to false smut in greenhouse showed that 3 varieties were middle resistance (MR), such as ‘FJZD10-5’,‘FJZD10-8’, 4 varieties for middle sensibility (MS) and 8 varieties for sensibility (S). However, the results of rice resistance to false smut in field showed 11 varieties for middle resistance (MR), such as ‘FJZD10-2’,‘FJZD10-3’, and 4 varieties for middle sensibility (MS). The evaluation results of rice resistance to neck blast in field showed that 7 varieties were middle resistance (MR), such as ‘FJZD10-6’,‘FJZD10-7’, and 4 varieties for middle sensibility(MS) and 4 varieties for sensibility (S). The frequency of rice varieties co-infected by rice false smut and neck blast in tested field of Jianyang,Ninghua and Fu'an were 100%, 73.33% and 73.33% respectively. Among the 15 rice varieties, no any variety showed resistance to both rice false smut and neck blast disease.
Correlation Analysis of Adult Resistance Evalution Factors to Strip Rust in Wheat Cultivars and Its Clustering
PENG Yu-jiao, KANG Xiao-hui, CHENG Deng-fa, SUN Jing-rui, WU Guo-ping, BAI Xue
2012, 27(1): 48-54.
Abstract:
Adult resistance evalution and the correlation between the factors were studied by natural inoculation, and cluster was also investigated based on 241 wheat cultivars which comes from the wheat growing regions of China in this paper.The correlation among the three resistance components indicates that there is positively relationship between infected type was and finally disease index and prevail rate.the results show there was 39 high resistance cultivars by resistance identifying and clustering (16.18%), 111 middle resistance cultivars (46.05%), 68 middle susceptibility cultivars (28.21%), 23 high susceptibility cultivars (9.54%), respectively.
The Influence of Temperature and Humidity on the Population Dynamics of Small Green Leafhopper at Tea Garden
LI Hui-ling, LIN Nai-quan
2012, 27(1): 55-59.
Abstract:
The paper discusses the population dynamics of the adult, nymph and egg density of small green leafhopper (SGL), the influence of temperature and humidity on the population dynamics of the SGL at the tea garden of Fujian Agriculture & Forestry University, Jinshan, Fuzhou, Fujian, 2007. The results showed:①The population dynamics of the SGL had two peaks, a significant positive correlation was shown among the population dynamics of the egg, nymph and adult of the SGL.②The population dynamics of the adult, nymph and egg density of the SGL was significantly affected by the temperature during the occurrence. While the average temperature above 28℃and lower 20℃ condition, the population dynamics of the SGL were declined rapidly.③The temperature for the dynamics of the adult, nymph population and egg density of the SGL were coincided with the Yield Density model.
Composition and Structure of the Arthropod Community in the Mango Field
LI Jian-yu, DONG Tie-sheng, FU Jian-wei, YOU Yong, ZHANG Li, ZHAN Zhi-xiong
2012, 27(1): 60-66.
Abstract:
The composition and structure of arthropod community in mango field were investigated in this study, and the characterization of arthropod was also described. They will enrich the theoretical foundation for ecological of pests in mango orchard. The composition and diversity of arthropod communities in mango orchards were investigated in Hui'an country, Fujian Province, China in this study. The results showed that all the collected arthropod individuals belong to 2 classes, 17 orders, 80 families and 203 species. Among them, 106 species were herbivores (52.22%), 31 species were predators and parasitoids (15.27%), 14 species were detritivores (6.90%) and 52 species were spiders (25.62%). The main dominant herbivores were Tropidothorax elegans Distant and Recilia dorsalis (Motschlsky), and the main dominant predators and parasitoids were Tetrastichus coccinellae Kurju-mov and Tetramorium guineense Fabricius, and the main dominant detritivores was Musicdae, and the main dominant spiders was Phintella sp.1.
Occurrence of Longicorn on Plum Trees and Their Comparison of Host preference to Three Kinds of Plum Trees
HUANG Peng, YU De-yi, WANG Jin-ming, YAO Jin-ai, FANG Da-lin
2012, 27(1): 67-70.
Abstract:
The author surveyed the the damage and species of longicorn occurred on plum trees,compared their host preference to Furong plum, Dahong plum and black plum.The result showed that the plum trees were easly to subjected to the adults and larvae, contained 14 species of 11genera.The main species of longicorn to caused damage to the three kinds of plum trees were Anoplophora chinensis, Aromia bungii and Apriona germari. For the host preference to the tree kinds of plum trees, black plum was the strongest, Furong plum was the second and the Dahong plum was the weakest.
Effects of Initial pH Value for the Fermentation of Brevibacillus brevis FJAT-0809-GLX on the Generation of Secondary Metabolites Substances
CHEN Zheng, LIU Bo, ZHU Yu-jing, HU Gui-ping, CHE Jian-mei, TANG Jian-yang
2012, 27(1): 71-76.
Abstract:
Components of FJAT-0809-GLX fermentation liquid in10 different initial pH values were analyzed by GC-MS. Eleven compounds with the match degree above 90% were identified. The compounds found commonly in these 10 fermentation liquid was Hexahydro-pyrrolo pyrazine-1,4-dione.There were two sorts of functional compounds in the FJAT-0809-GLX fermentation liquids; one is volatile compounds which relate to the special aroma of this fermentation liquid, including 5-methyl-2-Furancarboxaldehyde,Maltol,Benzeneacetaldehyde, n-Hexa-decanoic acid, Oleic Acid, and 2-Hydroxy-3-methyl-2-cyclopenten-1-one. The other sort of functional compound had antiseptic and bacteriostatic effect, including Maltol, 2-Furanmethanol and Hexahydro-pyrrolo pyrazine-1,4-dione and 5-(hydroxymethyl)-2-Furancarboxaldehyde. The fermentation liquid with the initial pH value of 6 to 12 had inhibitory activity against K88.The pH value 7 had the best inhibitory activity; the diameter of inhibition zone was 20.33mm. Different initial pH values led to different terminal pH value, and then affect the generation properties of secondary metabolites of Brevibacillus brevis FJAT-0809-GLX.
Discriminate Model of Electronic Nose for Distinguishing Volatiles of Microbial Fermentation Bed in Swine House
LAN Jiang-lin, LIU Bo, CHEN Zheng, SONG Ze-qiong, LIN Juan
2012, 27(1): 77-86.
Abstract:
Volatile components from microbial fermentation bed in pig farming were analyzed with gas chromatography-mass spectrometry, results show that 22 components are separated and identified in 30 samples(matching degree≥90,relative content≥4%), in which based on the kind and content in the components 4 types were clustered, corresponding to 4 ferment level respectively e.g. First level was characterized with shallaw degree of fermentation;Second level with medium degree;Third level with deep degree;Fourth level with profound. Using cluster analysis and discrimination analysis to build up the discrimination model.The accurate rate for discrimination model was up to 96.67%. utilizing the mathematical equation to determine the fermentation index of unknown pig litter, the degree of fermentation of unknown litter were judged fastly and accurately.The model of ferment level for pig litter was built up to provide the experience for computer-based standardizing analysis for scientific management of Pig Litter in the Microbial Fermentation Bed, further more with the new idea for pig breeding.
Distribution Characteristics of Soil Aggregates and Organic Carbon in Hill Tea Plantation
WANG Feng, WANG Yi-xiang, JANG Fu-ying, WU Zhi-dan, YOU Zhi-ming, ZHANG Wen-jin, WENG Bo-qi
2012, 27(1): 87-92.
Abstract:
Distribution of soil aggregates and organic carbon were analyzed in hill tea plantation by the methods of field investigation and laboratory analysis. The result indicated that the soil granule structure of tea plantation was dominated by >5 mm aggregate for 0-20cm soil layers, accounting for 20.59%, the soil granule structure of tea plantation was dominated by 5 mm were the highest for both 0-20 cm and 20-40 cm soil layers, and the content of organic carbon increased with increment of aggregate size. The reserve and distribution of the ratio of organic carbon in >5 mm aggregate in 0-20 cm were the highest and in 0.25-0.5 mm were the lowest. On the contrary, the reserve and distribution of the ratio of organic carbon in 0.25-0.5 mm aggregate in 20-40 cm were the highest and in >5 mm were the lowest.
A Leaping Forward Required for Development of Modern Multi-storied Agriculture
WENG Bo-qi, LIU Rong-zhang, LIU Ming-xiang, ZHENG Bai-long
2012, 27(1): 93-98.
Abstract:
The paper has historically reviewed the development and practice of multi-storied agriculture in Fujian. Based on the new challenges faced, it has been suggested in the paper that the innovation in strategies and techniques of development are essential and urgent for modern multi-storied agriculture to achieve the practical model in the light of local conditions. Innovational techniques in recyclable agriculture, low-carbon agriculture, green agriculture, facility agriculture and precision agriculture are the key of optimizations to establish a comprehensive multi-storied agriculture system in Fujian as well as southern region in China.
Construction and Empirical Analysis of the Evaluation System of Leisure agriculture base on Fuzzy Comprehensive
CHEN Zhi-feng, ZHENG Bai-long, XU Biao-wen, ZENG Yu-rong
2012, 27(1): 99-103.
Abstract:
Leisure agriculture as a modern product of rapid economic development, in order to promote the sound development of it, This article Establish evaluation index system of agricultural leisure from the environmental characteristics, product characteristics, management, infrastructure development, behavior and emotional experience, then through the establishment of fuzzy comprehensive evaluation model and empirical analysis, Good results have been achieved and with relatively strong pertinence and maneuverability, provideing a feasible evaluation method for the evaluation of leisure agriculture, and promoting the further development of leisure agriculture evaluation system.
Research Progress of Mite Species on Mushroom
LAN Qing-xiu, LU Zheng-hui, FAN Qing-hai
2012, 27(1): 104-108.
Abstract:
Pest mites often cause economic damage to mushroom,the researches on the mite species occurring on mushroom in China with highlight on Fujian province were reviewed, all of mites belonged to 29 familes in China and 52 species, 23 familes in Fujian, elaborated predatory mites applied research on mushroom in domestic and international, discussed current problems and research prospects of controlling technology also, in order to provide a theoretical basis for pest mites control and beneficial mite application.
GC-MS Analyses for Volatile Components of Syzygium samarangense
ZHANG Li-mei, XU Ling, CHEN Zhi-feng, YU Dong, WEI Xiu-qing, ZHANG Xi-juan, CHEN Zheng, XU Jia-hui,
2012, 27(1): 109-112.
Abstract:
Volatile components of wax apple named "Nongke II" were extracted from the ripe fruit with headspace solid-phase micro-extraction method and identified with gas chromatography-mass spectrometry (GC-MS). The results showed that twelve fragrant components were identified. Among the fragrant components, six terpenes were account for 63.74% of the total content, the alcohol, aldehyde, lipid and furan were account for 22.48%, 0.48%, 4.68% and 4.44%, respectively.Nine compounds were identified with the match degree above 90% and relative amount of 93.46%.The main aromas were cis-3-nonene-1-ol, (-)-Alpha-Cubeb oil terpenes and trans-caryophyllene.