1987 Vol. 2, No. 1
Display Method:
1987, 2(1): 1-9.
Abstract:
The rice stem borer, Tryporyza incertulas Walker, is a major rice pest in Fujian.It usually makes a yield loss of 5-10%.Since it is polyvoltine insect, it multiplies four to five generations each year in south Fujian. And the occurring time and quantity in various generations change with seasons and places.In the research, according to the classical demand on the forecast factors for the rice stem borer, 68-82 factors of the occurring time and 83-94 facetrs of the occurring quantity were selected as the parameters of forecast equation.Then the computer IBM-pc/xt was used for polyre gression to build up the forecast equation as follows: yx=a0x0+a1x1+…+anxn(yx Where yx = is the occurring time or quantity, an the coefficient, and Xn the forecast factor. Simultaneously IPM experience of various forecast stations was programmed and put into computer, as reference data on controlling the pest.Two years of practice proved that the rate of exact forecast was 57.14% and the rate of conformable forecast was 92.9% within the range of allowable error.
The rice stem borer, Tryporyza incertulas Walker, is a major rice pest in Fujian.It usually makes a yield loss of 5-10%.Since it is polyvoltine insect, it multiplies four to five generations each year in south Fujian. And the occurring time and quantity in various generations change with seasons and places.In the research, according to the classical demand on the forecast factors for the rice stem borer, 68-82 factors of the occurring time and 83-94 facetrs of the occurring quantity were selected as the parameters of forecast equation.Then the computer IBM-pc/xt was used for polyre gression to build up the forecast equation as follows: yx=a0x0+a1x1+…+anxn(yx Where yx = is the occurring time or quantity, an the coefficient, and Xn the forecast factor. Simultaneously IPM experience of various forecast stations was programmed and put into computer, as reference data on controlling the pest.Two years of practice proved that the rate of exact forecast was 57.14% and the rate of conformable forecast was 92.9% within the range of allowable error.
1987, 2(1): 10-15.
Abstract:
The ultrastructure of Azolla filiculoides was investigated by means of transmission and scanning electron microscopy .Hairs, epidermal tricho-mes, were found on the shoot tip, the mature leaf cavity and the embryo.The hair cells,during various developing stages,had some of the features transfer cells had.And the hair cells on the shoot tip and the embryo had the structural features secretory cells had. In their cytoplasm, there were abundant mitochodria, dictyosomes, rough endoplasmic reti-culum,vacuoles and secretory granules.From this it is inferred that such hair cells might be involved in supplying the nutrient substances to the non N-fixing vegtative cells and the akinets of Anabacna; that the hair cells in the mature leaf cavity might play an important role in metabolic exchange between Azolla and Anabaena; and that the difference in structure between the terminal and the basal cells might involve their difference in function.
The ultrastructure of Azolla filiculoides was investigated by means of transmission and scanning electron microscopy .Hairs, epidermal tricho-mes, were found on the shoot tip, the mature leaf cavity and the embryo.The hair cells,during various developing stages,had some of the features transfer cells had.And the hair cells on the shoot tip and the embryo had the structural features secretory cells had. In their cytoplasm, there were abundant mitochodria, dictyosomes, rough endoplasmic reti-culum,vacuoles and secretory granules.From this it is inferred that such hair cells might be involved in supplying the nutrient substances to the non N-fixing vegtative cells and the akinets of Anabacna; that the hair cells in the mature leaf cavity might play an important role in metabolic exchange between Azolla and Anabaena; and that the difference in structure between the terminal and the basal cells might involve their difference in function.
1987, 2(1): 16-24.
Abstract:
In the rice-azolla-fish cropping system as we tested after azolla had been eaten by fish, the effect of fish’s excreta for rice growth and nitrogen uptake were studied with 15N isotope labelled method. Results of experiment in the paddy fields showed that azolla was a good fodder to fish (T,nilotica). Its weight increased 41.2-48.03% during early rice season, there azolla(60 kg/ha) was put into rice-azolla-fish system as the fish’s fodder.After that, the excreta also fertilized the paddy soil and had a good effect to rice growth so the rice yield increased 9.7-20.7% than that of check (without application of N-fertilizer), but azolla (60kg/ha) was incorporated as green manure in paddy soil the rice yield only increased 4.4-14.8% as compared with check. we found azolla-N could be utilized for several times in rice-azolla-fish. First the nitrogen content of fish reached 2.41% and 15N excess was changed from 0.366% to 0.4313%. So the protein of plant was changed into animal protein effectively during early rice seaeon. Second, the rates of 15N recoveried by fish and rice were 38.24% and 29.52% respectively, there fore the total rate of 15N utilized was 67.76% in the 15N isotope labelled azolla as fish fodder and that was higher than the average rate of N utilized in azolla incorporated as green manure. The later was 48.83%.
In the rice-azolla-fish cropping system as we tested after azolla had been eaten by fish, the effect of fish’s excreta for rice growth and nitrogen uptake were studied with 15N isotope labelled method. Results of experiment in the paddy fields showed that azolla was a good fodder to fish (T,nilotica). Its weight increased 41.2-48.03% during early rice season, there azolla(60 kg/ha) was put into rice-azolla-fish system as the fish’s fodder.After that, the excreta also fertilized the paddy soil and had a good effect to rice growth so the rice yield increased 9.7-20.7% than that of check (without application of N-fertilizer), but azolla (60kg/ha) was incorporated as green manure in paddy soil the rice yield only increased 4.4-14.8% as compared with check. we found azolla-N could be utilized for several times in rice-azolla-fish. First the nitrogen content of fish reached 2.41% and 15N excess was changed from 0.366% to 0.4313%. So the protein of plant was changed into animal protein effectively during early rice seaeon. Second, the rates of 15N recoveried by fish and rice were 38.24% and 29.52% respectively, there fore the total rate of 15N utilized was 67.76% in the 15N isotope labelled azolla as fish fodder and that was higher than the average rate of N utilized in azolla incorporated as green manure. The later was 48.83%.
1987, 2(1): 25-31.
Abstract:
The change in urease activity in 4 types of paddy soil, i.e. percogenic paddy soil, submergic paddy soil, sandy clay soil and gleyed paddy soil, was studied. And the relationship between the activity of urease and the efficiency of urea in the soil types was analysed. Results showed that the urease activity of soil types was different from each other’s, and changed with soil profile,season, method of fertilizer application, number of urease-producing microorganisms as well as the total nitrogen of soil. Of the 4 soil types, percogenic paddy soil had the highest urease activity and the lowest efficiency of urea; gleyed paddy soil had the lowest urease activity and the highest efficiency of urea; submergic paddy soil lay between the above two in both the activity of urease and the efficiency of urea.The change in urease activity in different paddy soil showed a tendency that activity was higher in early cropping season than in late cropping season; in top soil layer than in low soil layer.The soils where urea or other fertilizers was applied with organic manure increased their activity of urease as compared with the control treatment.
The change in urease activity in 4 types of paddy soil, i.e. percogenic paddy soil, submergic paddy soil, sandy clay soil and gleyed paddy soil, was studied. And the relationship between the activity of urease and the efficiency of urea in the soil types was analysed. Results showed that the urease activity of soil types was different from each other’s, and changed with soil profile,season, method of fertilizer application, number of urease-producing microorganisms as well as the total nitrogen of soil. Of the 4 soil types, percogenic paddy soil had the highest urease activity and the lowest efficiency of urea; gleyed paddy soil had the lowest urease activity and the highest efficiency of urea; submergic paddy soil lay between the above two in both the activity of urease and the efficiency of urea.The change in urease activity in different paddy soil showed a tendency that activity was higher in early cropping season than in late cropping season; in top soil layer than in low soil layer.The soils where urea or other fertilizers was applied with organic manure increased their activity of urease as compared with the control treatment.
1987, 2(1): 32-38.
Abstract:
Years after cultivation, the hybrid combinations Shanyou No.2 and Siyou No.2 were gradually losing their resistance to blast because their restorer line IR24 was susceptible. In order to solve this probelm.a new restorer line Minghui 63 was produced by crossing IR 30 and Gui 630; Shanyou 63 by crossing restorer line Minghui 63 and ms line Zhenshan 97A. Reasults showed that this combination was marked by higher yield, better grain quality, more resistance to blast and extensive adaptability.It had extended rapidly, ranking first on the list in the planting area. In 1986 it covered 2. 26 million hectares in the south of China. Between 1982 and 1985, it usually took the lead in area whether in the country or in local districts; the average yield from 1982-1985 was 7.2T/ ha, increasing by 1.02T/ha or 16.1% as compared with the check combinations Shanyou No.2 and Shanyou No.6. It is estimated that the total yield increased by 1986 amounted 2.89 million tons. This combination was especially fit to grow as a medium variety because its growth period lasts 4-5 days longer than that of Shanyou No.2. If it is used as a late variety in the double cropping system, the sowing date should be taken into considerations in order to avoid the autumn cool. It is estimated that this combination will exten its planting area in the south of China and develop its social efficiency. And the other new combinations with the restorer line Minghui 63 are being experimented with and are under development.
Years after cultivation, the hybrid combinations Shanyou No.2 and Siyou No.2 were gradually losing their resistance to blast because their restorer line IR24 was susceptible. In order to solve this probelm.a new restorer line Minghui 63 was produced by crossing IR 30 and Gui 630; Shanyou 63 by crossing restorer line Minghui 63 and ms line Zhenshan 97A. Reasults showed that this combination was marked by higher yield, better grain quality, more resistance to blast and extensive adaptability.It had extended rapidly, ranking first on the list in the planting area. In 1986 it covered 2. 26 million hectares in the south of China. Between 1982 and 1985, it usually took the lead in area whether in the country or in local districts; the average yield from 1982-1985 was 7.2T/ ha, increasing by 1.02T/ha or 16.1% as compared with the check combinations Shanyou No.2 and Shanyou No.6. It is estimated that the total yield increased by 1986 amounted 2.89 million tons. This combination was especially fit to grow as a medium variety because its growth period lasts 4-5 days longer than that of Shanyou No.2. If it is used as a late variety in the double cropping system, the sowing date should be taken into considerations in order to avoid the autumn cool. It is estimated that this combination will exten its planting area in the south of China and develop its social efficiency. And the other new combinations with the restorer line Minghui 63 are being experimented with and are under development.
1987, 2(1): 39-44.
Abstract:
Different rice varieties have different responsibilities to various potassium concentrations. This paper reports a water culture experiment, conducted in 1985, with different potassium concentraions, giving prominence to the agronomic characters, the agrochemical and biochemical features as well as the sensitivity to low potassium concentrations. It makes a comparative study of the characteristics of rice varieties with degrees of tolerance of low potassium.Results showed that the rice varieties which were possessed of strong tolerance had remarkable adaptability to low potassium surroundings. They took up 30% more potassium than those which were not possessed of strong tolerance. Thus the above indexes in question of rice varieties. differing in tolerance showed significant diversity, esp. in the root system.Take Kefuhong No. 2 for example: its vitality of the root system, relative plant height above ground and relative biomass far surpassed those of the sensitive variety Xiulingxun. These results showed that the characters of tolerant varieties were closely related to the growth of the root system.
Different rice varieties have different responsibilities to various potassium concentrations. This paper reports a water culture experiment, conducted in 1985, with different potassium concentraions, giving prominence to the agronomic characters, the agrochemical and biochemical features as well as the sensitivity to low potassium concentrations. It makes a comparative study of the characteristics of rice varieties with degrees of tolerance of low potassium.Results showed that the rice varieties which were possessed of strong tolerance had remarkable adaptability to low potassium surroundings. They took up 30% more potassium than those which were not possessed of strong tolerance. Thus the above indexes in question of rice varieties. differing in tolerance showed significant diversity, esp. in the root system.Take Kefuhong No. 2 for example: its vitality of the root system, relative plant height above ground and relative biomass far surpassed those of the sensitive variety Xiulingxun. These results showed that the characters of tolerant varieties were closely related to the growth of the root system.
1987, 2(1): 45-49.
Abstract:
The parthenogenetic ovaries induced by chemicals (PCPA) in vitro were hybride rice (indica) Fl’s.when they were cultured for 7-15 on SK3 and N6 media supplemented with GA3 0.5-2 ppm,the parthenogenetic plantlets of five combinations,such as Siyou No.30, were obtained.The regeneration rate of 5-10-day-old parthenogenetic ovaries grown on SK, and N6 was higher than that on MS. At a range of 0,5-2ppm, GA3 was able to induce parthenogenetic ovaries to form plantlets directly; NAA and 2, 4-D, to form calli.But KT and TRIA were not able to,Most of the parthenogenetic plantlets developed directly out of embryos: a few parthenogenetic ovaries first formed calli and then regenerated into green plantlets after their transfer on MS. Some of the parthenogenetic plantlets were haploids,others were natural diploids. Parthenogenetic plants showed their relative regu-lartiy and consistence in main argronomic traits during the PO2-PO3 generations.
The parthenogenetic ovaries induced by chemicals (PCPA) in vitro were hybride rice (indica) Fl’s.when they were cultured for 7-15 on SK3 and N6 media supplemented with GA3 0.5-2 ppm,the parthenogenetic plantlets of five combinations,such as Siyou No.30, were obtained.The regeneration rate of 5-10-day-old parthenogenetic ovaries grown on SK, and N6 was higher than that on MS. At a range of 0,5-2ppm, GA3 was able to induce parthenogenetic ovaries to form plantlets directly; NAA and 2, 4-D, to form calli.But KT and TRIA were not able to,Most of the parthenogenetic plantlets developed directly out of embryos: a few parthenogenetic ovaries first formed calli and then regenerated into green plantlets after their transfer on MS. Some of the parthenogenetic plantlets were haploids,others were natural diploids. Parthenogenetic plants showed their relative regu-lartiy and consistence in main argronomic traits during the PO2-PO3 generations.
1987, 2(1): 50-56.
Abstract:
As a new variety of sweet potato, Minkang 329 was produced by crossing Pushu 125 and Nanhui No. 3. The 1981-1935 green-house tests for 66 different virulent strains of sweet potato bacterial wilt (Pseadomonas solanacearum) showed that the mean index of disease infection was 7.4.In the field conditions, the death rate of seeding was 0.8%. In the field plots the mean index of disease infection was 2.5; in the diseased plots, 4.7 : lower than that of the local check variety Xinzhonghua. The results also indicated that Mingkang 329 matched China’s check variety Huabei 48 in the resistance to sweet potato bacterial wilt.Tests for its productivity in 72 diseased areas showed that the yield of Mingkang 329 was 31.6% higher than that of Huabei 48, 2.8-2.9 times that of Xinzhonghua. But in 53 disease-free areas and 23 yield estimating plots of Fujian Province, the yield of Minkang 329 increased by 10.8 -26.6% as compared with Xinzhonghua.Minkang 329 formed its tuber early. 25 days after transplantation, the tuber was formed out of young roots. The yield reached 3050 kg. per mu in 120 days.Under better cultivation,the average yield was more than 4500 kg. whether in mountain districts, plains or coastal sandy areas.From 1934 to 1986, Minkang 329 was introduced to 40 counties in Fujian. The accumulative cultivation area reached 800 thousand mu.The in-creased output altogether was more than 100 million kg. The social efficiency was over 30 million yuan. This variety was also introduced to the neibouring provinces and also showed higher yield in an area of 10 thousand mu.
As a new variety of sweet potato, Minkang 329 was produced by crossing Pushu 125 and Nanhui No. 3. The 1981-1935 green-house tests for 66 different virulent strains of sweet potato bacterial wilt (Pseadomonas solanacearum) showed that the mean index of disease infection was 7.4.In the field conditions, the death rate of seeding was 0.8%. In the field plots the mean index of disease infection was 2.5; in the diseased plots, 4.7 : lower than that of the local check variety Xinzhonghua. The results also indicated that Mingkang 329 matched China’s check variety Huabei 48 in the resistance to sweet potato bacterial wilt.Tests for its productivity in 72 diseased areas showed that the yield of Mingkang 329 was 31.6% higher than that of Huabei 48, 2.8-2.9 times that of Xinzhonghua. But in 53 disease-free areas and 23 yield estimating plots of Fujian Province, the yield of Minkang 329 increased by 10.8 -26.6% as compared with Xinzhonghua.Minkang 329 formed its tuber early. 25 days after transplantation, the tuber was formed out of young roots. The yield reached 3050 kg. per mu in 120 days.Under better cultivation,the average yield was more than 4500 kg. whether in mountain districts, plains or coastal sandy areas.From 1934 to 1986, Minkang 329 was introduced to 40 counties in Fujian. The accumulative cultivation area reached 800 thousand mu.The in-creased output altogether was more than 100 million kg. The social efficiency was over 30 million yuan. This variety was also introduced to the neibouring provinces and also showed higher yield in an area of 10 thousand mu.
1987, 2(1): 57-66.
Abstract:
The feed formulas and feeding standards to meet the nutrient requirements of the Arbor Acres and Redbro chickens raised in Fuzhou area were investigated according to the climatic condition and available feed-resources, especially based on the nutrient compositions of feeds usually used there. By the help of the technology of micro computer and the references of feeding standards for these two breeds, twenty-five formulas of three kinds of energy and protein levels were calculated to be nutritionally adequate during starter (1 to 30 days of age) grower (31 to 49 days of age) and finisher (50 to 56 days of age) phases. All experimental feed ingredients were supplied by the local feed company. Three feeding trials were conducted with 15,000 chickens. After 56 days’ feeding, the results showed that: (1) in the group of high nutrition level, the average body weight and gain-feed ratios of Arbor Acres were 1.98 to 2.23 kg and 1 : 1.99 to 1 : 2.28 respectively and those of Redbro were 1.70 to 1.94 kg and 1:2.06 to 1:2.27 rexpectively; (2) in the group of middle levei, those of Arbor Acres were 1.96 to 2.15 kg and 1 : 2.04 to 1 : 2.23 respectively, and those of Redbro were 1.67 to 1.91 kg and 1 : 2.1.3 to 1 : 2.30 respectively; (3) in the group of low level, those of Arbor Acres were 1.80 to 2.02 kg and 1 : 2.27 to 1 : 2.39 respectively, and those of Redbro were 1.56 to 1.77 kg and 1 : 2.27 to 1 :2.50 respectively.The statistical analysis indicated that the net gain of either Arbor Acres or Redbro was no significant difference (P0.05) between the high and middle level, but the significant difference (p 0.01) between the high and low level was found and the difference between the middle and low level was significant (p0.05) or very significant (p0.01) . Summing up the results of metabolic test, carcass measurement, comparative slaughter assay and L16(43) orthogonal experimental design in the group of middle nutrition level, we suggested that the best formula composition was formula No.9 in starter period, No.8 in grower and No.4 in finisher. It was named the fourth formula of broiler chicken’s mixed feed. Through 2,000, 000 chickens feeded in Fuzhou area, we concluded that the mixed feed produced with the fourth formula had more advantages of well-gainad weight and high feed conversion ratio than other formulas for broilers.
The feed formulas and feeding standards to meet the nutrient requirements of the Arbor Acres and Redbro chickens raised in Fuzhou area were investigated according to the climatic condition and available feed-resources, especially based on the nutrient compositions of feeds usually used there. By the help of the technology of micro computer and the references of feeding standards for these two breeds, twenty-five formulas of three kinds of energy and protein levels were calculated to be nutritionally adequate during starter (1 to 30 days of age) grower (31 to 49 days of age) and finisher (50 to 56 days of age) phases. All experimental feed ingredients were supplied by the local feed company. Three feeding trials were conducted with 15,000 chickens. After 56 days’ feeding, the results showed that: (1) in the group of high nutrition level, the average body weight and gain-feed ratios of Arbor Acres were 1.98 to 2.23 kg and 1 : 1.99 to 1 : 2.28 respectively and those of Redbro were 1.70 to 1.94 kg and 1:2.06 to 1:2.27 rexpectively; (2) in the group of middle levei, those of Arbor Acres were 1.96 to 2.15 kg and 1 : 2.04 to 1 : 2.23 respectively, and those of Redbro were 1.67 to 1.91 kg and 1 : 2.1.3 to 1 : 2.30 respectively; (3) in the group of low level, those of Arbor Acres were 1.80 to 2.02 kg and 1 : 2.27 to 1 : 2.39 respectively, and those of Redbro were 1.56 to 1.77 kg and 1 : 2.27 to 1 :2.50 respectively.The statistical analysis indicated that the net gain of either Arbor Acres or Redbro was no significant difference (P0.05) between the high and middle level, but the significant difference (p 0.01) between the high and low level was found and the difference between the middle and low level was significant (p0.05) or very significant (p0.01) . Summing up the results of metabolic test, carcass measurement, comparative slaughter assay and L16(43) orthogonal experimental design in the group of middle nutrition level, we suggested that the best formula composition was formula No.9 in starter period, No.8 in grower and No.4 in finisher. It was named the fourth formula of broiler chicken’s mixed feed. Through 2,000, 000 chickens feeded in Fuzhou area, we concluded that the mixed feed produced with the fourth formula had more advantages of well-gainad weight and high feed conversion ratio than other formulas for broilers.
1987, 2(1): 67-71.
Abstract:
A study on the elimfnation of citrus Huanglungbin pathogen by shoot tip grafting (STG) in vitro was made from 1983 to 1986. The result showed that 3 budlings by STG were obtained from 85 Huanglungbin takan seedlings. The STG budlings and original diseased Pankan seedlings (control) were indexed for the presence of Huanglungbin pathogen respectively by the methods of biological test and electron microscope examination. The indicated Ponkan seedlings inoculated with the buds from STG budlings did not show any Huanglungbin symptoms, and in the sieve cells of leaf vein of indicated seedlings no Huanglungbin pathogen was discovered under electron microscops. On the other hand, the typical symptoms appeared on the indicated Ponkan seedlings inoculated with the buds of control plant after 5-9 months and many typical pathogens (rickettsia-like or bacteria-like organisms) were found in their sieve cells of leaf vein under electron microscope. These results showed that the Huanglungbin pathogen could be eliminated by STG.
A study on the elimfnation of citrus Huanglungbin pathogen by shoot tip grafting (STG) in vitro was made from 1983 to 1986. The result showed that 3 budlings by STG were obtained from 85 Huanglungbin takan seedlings. The STG budlings and original diseased Pankan seedlings (control) were indexed for the presence of Huanglungbin pathogen respectively by the methods of biological test and electron microscope examination. The indicated Ponkan seedlings inoculated with the buds from STG budlings did not show any Huanglungbin symptoms, and in the sieve cells of leaf vein of indicated seedlings no Huanglungbin pathogen was discovered under electron microscops. On the other hand, the typical symptoms appeared on the indicated Ponkan seedlings inoculated with the buds of control plant after 5-9 months and many typical pathogens (rickettsia-like or bacteria-like organisms) were found in their sieve cells of leaf vein under electron microscope. These results showed that the Huanglungbin pathogen could be eliminated by STG.
1987, 2(1): 72-77.
Abstract:
In this paper, the world’s chief achievements in the mutation breeding of crops, including the development of new crop mutation varieties and new mutants, have been summarized and discussed. As regards new crop mutation varieties, the conditions and characters of crop mutation breeding in various countries,the inducing mutation conditions of various crops, the different mutagens and the improved characters have been analysed.As regards various mutants, their types, such as short culm, early maturity,good quality, resistanced to disease, plant morphology, especial character have been dealt with.Again,the author describes the trends of mutation breeding,by giving an account of the object of mutation breeding, the selection of radiation material, the indirect use of mutants, the repeat treatment by physical and chemical means,the development of orogagated plant,the combination of inducing mutation and in-vitro biotechnique.
In this paper, the world’s chief achievements in the mutation breeding of crops, including the development of new crop mutation varieties and new mutants, have been summarized and discussed. As regards new crop mutation varieties, the conditions and characters of crop mutation breeding in various countries,the inducing mutation conditions of various crops, the different mutagens and the improved characters have been analysed.As regards various mutants, their types, such as short culm, early maturity,good quality, resistanced to disease, plant morphology, especial character have been dealt with.Again,the author describes the trends of mutation breeding,by giving an account of the object of mutation breeding, the selection of radiation material, the indirect use of mutants, the repeat treatment by physical and chemical means,the development of orogagated plant,the combination of inducing mutation and in-vitro biotechnique.
1987, 2(1): 78-83.
Abstract:
Some strawberry, melon, bean, orange,litchi and water spinach were analyzed for ascorbic acid content by high-performance liquid chor-matography (HPLC) with a UV detector at 254nm. The results were com-pared with the dyetitration method using 2.6-dichlorophenol-indophenol and the values of ascorbic acid by both HPLC and titration were comparable. The mobile phase was made of 0.008 M phosphate buffer (pH-5.0) with 0.002M tetrabtuyl ammonium hydroxide. Extraction procedures using 3% citric acid were compared with those using acetic acid and me-taphosphoric acid.The recovery values were more than 90% and minimum detectable quantity was 0.05μg. The study showed that HPLC was a simple, rapid, sensitive method for the analysis of ascorbic acid content in fruits and vegetables. The highly variable of ascorbic acid between bean and litchi varieties was obtained. Authers suggest that the variety with high content of ascorbic acid must be used as parent plant in future breeding work.
Some strawberry, melon, bean, orange,litchi and water spinach were analyzed for ascorbic acid content by high-performance liquid chor-matography (HPLC) with a UV detector at 254nm. The results were com-pared with the dyetitration method using 2.6-dichlorophenol-indophenol and the values of ascorbic acid by both HPLC and titration were comparable. The mobile phase was made of 0.008 M phosphate buffer (pH-5.0) with 0.002M tetrabtuyl ammonium hydroxide. Extraction procedures using 3% citric acid were compared with those using acetic acid and me-taphosphoric acid.The recovery values were more than 90% and minimum detectable quantity was 0.05μg. The study showed that HPLC was a simple, rapid, sensitive method for the analysis of ascorbic acid content in fruits and vegetables. The highly variable of ascorbic acid between bean and litchi varieties was obtained. Authers suggest that the variety with high content of ascorbic acid must be used as parent plant in future breeding work.
1987, 2(1): 84-87.
Abstract:
1987, 2(1): 88-91.
Abstract:
1987, 2(1): 92-95.
Abstract:
1987, 2(1): 96-97.
Abstract:
