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Volume 2 Issue 1
Apr.  2012
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Chen Jiong, Song Ruilin, Ke Chong. STUDY ON THE ELIMINAITON OF CITRUS HUANGLUNGBIN PATHOGEN BY SHOOT-TIP GRAFTING IN VITRO[J]. Fujian Journal of Agricultural Sciences, 1987, 2(1): 67-71.
Citation: Chen Jiong, Song Ruilin, Ke Chong. STUDY ON THE ELIMINAITON OF CITRUS HUANGLUNGBIN PATHOGEN BY SHOOT-TIP GRAFTING IN VITRO[J]. Fujian Journal of Agricultural Sciences, 1987, 2(1): 67-71.
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STUDY ON THE ELIMINAITON OF CITRUS HUANGLUNGBIN PATHOGEN BY SHOOT-TIP GRAFTING IN VITRO

  • Fruit Research Institute, Fujian Academy of Agricultural Sciences

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  • Received Date: March 09, 1987
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    • Abstract
  • A study on the elimfnation of citrus Huanglungbin pathogen by shoot tip grafting (STG) in vitro was made from 1983 to 1986. The result showed that 3 budlings by STG were obtained from 85 Huanglungbin takan seedlings. The STG budlings and original diseased Pankan seedlings (control) were indexed for the presence of Huanglungbin pathogen respectively by the methods of biological test and electron microscope examination. The indicated Ponkan seedlings inoculated with the buds from STG budlings did not show any Huanglungbin symptoms, and in the sieve cells of leaf vein of indicated seedlings no Huanglungbin pathogen was discovered under electron microscops. On the other hand, the typical symptoms appeared on the indicated Ponkan seedlings inoculated with the buds of control plant after 5-9 months and many typical pathogens (rickettsia-like or bacteria-like organisms) were found in their sieve cells of leaf vein under electron microscope. These results showed that the Huanglungbin pathogen could be eliminated by STG.
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    • References (5)
  • [3] 柯穗等,1986,通过苑丝子成功地将黄龙病病原从柑桔传到长春花。福建省农科院学报1(1):11~160
    [7] 赵学源等,1981,柑桔黄龙病对青霉素和四环素的反应。中国柑桔4:17.
    [8] 赵学源等,1983,利用茎尖嫁接脱毒培育无裂皮病的脐血橙。植物保护学报10(3):166.
    [11] Navarro, L, 1981.Citrus shoot-tip grafting in vitro(STG)and its application, A review,Proc, Int soc.Citriculture.452~456.
    [16] Nauer,E.M,,C,N,Roistacher,,T;L,Carson and T,nLurashige,1983,In vitro shoot-tip grafting to Eliminate citrus,viruses and virus-like pathogens produces uniform budlines, H ortscience, 18 (3):308~309.
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