Abstract: A disease with gosling liver bleeding as the main pathological syndrome occurred in Guangdong Province in 2012.A virus strain named GD-01 was isolated from a dead gosling,which could cause death on the duck embryo within 96 hours.8 days-old Muscovy ducklings infected with GD-01 showed the opisthotonos and liver bleeding before deuth.GD-01 was characterized by RT-PCR using specific primers for duck hepatitis virus A type 1,and an 199 bp fragment was amplified.Genetic analysis showed that the isolated strain belonged to duck hepatitis virus A type 1.