Purification and preliminary structure analysis of serum immunoglobulin in channel catfish (<i>Ictalurus punctatus</i>)

YI Guang-hui; LIN Tian-long; XIONG Bang-xi; LIU Xiao-dong; YANG Jin-xian

Volume 20 Issue 2

Apr. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2005 > 20(2): 91-96

YI Guang-hui, LIN Tian-long, XIONG Bang-xi, LIU Xiao-dong, YANG Jin-xian. Purification and preliminary structure analysis of serum immunoglobulin in channel catfish (Ictalurus punctatus)[J]. Fujian Journal of Agricultural Sciences, 2005, 20(2): 91-96.

Citation:

YI Guang-hui, LIN Tian-long, XIONG Bang-xi, LIU Xiao-dong, YANG Jin-xian. Purification and preliminary structure analysis of serum immunoglobulin in channel catfish (Ictalurus punctatus)[J]. Fujian Journal of Agricultural Sciences, 2005, 20(2): 91-96.

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Purification and preliminary structure analysis of serum immunoglobulin in channel catfish (Ictalurus punctatus)

1.
Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350013, China;
2.
Fisheries College, Huazhong Agricultural University, Wuhan, Hubei 430070, China

Received Date: 2003-10-15
Rev Recd Date: 2004-06-30
Publish Date: 2005-06-15

Abstract

Abstract

Serum immunoglobulin (Ig) from channel catfish (Ictalurus punctatus) immunized with Aeromonas so-bria was purified with ammonium sulfate precipitation followed by column chromatography.The results showed that serum Ig was mainly precipitated at 35%-55% ammonium sulfate saturation.Further purification by Sepharose-4B gel filtration and DEAE-52 anion exchange chromatography showed that the Ig from both of the columns all presented in the first protein peak.SDS-PAGE demonstrated that serum Ig was more pure after each step of purification.Structure analysis of the catfish serum Ig was performed by Western blot under denatured and reduced, denatured but non-reduced, non-denatured and non-reduced conditions using monoclonal and polyclonal antibodies as probes.The results showed that the molecular weight of the heavy chain was 72 kD, and the three distinct light chains were 21 kD, 23.5 kD, 26 kD respectively.The results suggested the heterogeneity were presented in channel catfish light chains.The serum Ig only had a molecular weight about 870 kD under non-reduced and non-denatured conditions.
- Ictalurus punctatus,
- Immunoglobulin,
- Purification,
- Structure analysis

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References(6)

References

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