Immunohistochemical localization of endocrine cells in the digestive tract of Pseudosciaena crocea

The immunohistochemical localization of endocrine cells in the digestive tract of Pseudosciaena crocea were studied with four mammal serum i. e. anti-5-HT, anti-SST, anti-Gas and anti-Glu by the immunohistochemical technique of the streptavidin-peroxidase marked with HRP. The results showed that the immunologically positive endocrine cells of 5-HT were distributed throughout the digestive tract, and the distributive density of 5-HT cell was higher throughout stomach, less in oesophagus and intestine. The immunologically positive endocrine cells of SST were located mostly in stomachus cardiacus, and stomachus fundus, but less in stomachus pyloricu, and only a few in the oesophagus, foregut and midgut, and it increased in hidgut. The immunologically positive endocrine cells of Gas were found in stomachus pyloricu, foregut and midgut, hidgut. It indicated that the cell density reduced from stomachus pyloricu to hidgut, but it was not found in oesophagus. stomachus cardiacus, and stomachus fundus. The immunologically positive endocrine cells of Glu were not found throughout the digestive tract. According to the morphologic character, the endocrine cells in the digestive tract of Pseudosciaena crocea were the same as other kinds of fish. It could be divided into two cell types, the opening and the closing types.