• 中文核心期刊
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Volume 26 Issue 4
Nov.  2011
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ZHU Chun-hua, JIANG Bin, LIU Bin-qiong, LIN Su, CHEN Zhen, HUANG Yu. Isolation and Preliminary Identification of H9 Subtype Influenza A Viruse[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 537-540.
Citation: ZHU Chun-hua, JIANG Bin, LIU Bin-qiong, LIN Su, CHEN Zhen, HUANG Yu. Isolation and Preliminary Identification of H9 Subtype Influenza A Viruse[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 537-540.
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Isolation and Preliminary Identification of H9 Subtype Influenza A Viruse

  • Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

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  • Received Date: May 03, 2011
  • Revised Date: June 02, 2011
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    • Abstract
  • 2011 two strains(FZ-04 and FZ-11)were isolated respectively from two broiler farms in Fujian.Hemagglutination test results showed that the isolated allantoic fluid can induce 1% chicken erythrocytes agglutination.Hemagglutination inhibition test results showed that the isolates allantoic fluids were only inhibited by avian influenza virus subtype H9 standard serum specificly.Specified and conserved M(Matrix)gene and HA(Hemagglutinin)gene of H9 subtype were amplified by RT-PCR with the segment lengths of 229bp and 732bp respectively.The results indicated that the isolated strains were H9 subtype of influenza A virus.The phylogenetic analysis of HA genes showed that isolates and representative strains CK/FJ/G9/09 have a closest relationship,belonging to the major sublineage CK/BJ/1/94 in China.
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  • 万春和,傅光华,程龙飞,等.A/Chicken/Zhejiang/H J/2007(H 9N2)禽流感病毒 8 基因测序及遗传进化分析[J]. 农业生物技术学报, 2009,(5):750-757.
    赵军,柴丽娜,王泽霖.1998～2008 年中国中部 H9N2 亚型 AIV 分离毒株 HA 基因的进化分析[J]. 病毒学报, 2011, 27(2):122-128.
    WU Z Q, JI J, ZUO K J, et al.Cloning and phylogenetic analysis of hemagglutinin gene of H9N2subtype avian influenza virus from different isolates in China during 2002to 2009[J]. Poult Sci,2010, 89(6):1136-1143.
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