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Volume 26 Issue 4
Nov.  2011
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HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Citation: HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
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Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX

  • Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian350003, China

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  • Received Date: January 01, 2011
  • Revised Date: June 18, 2011
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    • Abstract
  • Different extraction methods were compared in extracting of active substances from metabolites of Brevibacillus brevis FJAT-0809-GLX.It macroporous tesin method provided the best effect in extraction with 1.593 gL-1.Antibacterial activities of the extract was higher in it by macroporous resin method than it by the chloroform.The optimal procedures applied for extraction of active substances were: centrifuging the fermentation broth of FJAT-0809-GLX at 3600 rpm for 30 min,mixing the supernatant with 40 gL-1 macroporous resin(AmberliteXAD16)and then vortexing for 4h at 160 rpm,28℃;filling the column with the mixture and eluting it with acetone in the ratio of 3 times in given volum of extraction.The eluent was collected and concentrated rotary evaporation at 40℃.
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  • 李一青,刘树芳,李铭刚,等. 藤黄灰链霉菌 ECO00001 菌株中寡霉素 A 和 C 的分离鉴定及其活性[J]. 植物保护学报, 2008, 35(1):47-50.
    蔡寅,张芳,顾觉奋. 大孔吸附树脂在微生物制药分离纯化应用上的最新进展[J]. 离子交换与吸附, 2008, 24(5):473-480.
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