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ZHU Hai-xia, WAN Chu-he, HUANG Yu, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei. Cloning and Sequence Analysis of Muscovy Duck Interferon-Alpha[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 523-527.
Citation:
ZHU Hai-xia, WAN Chu-he, HUANG Yu, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei. Cloning and Sequence Analysis of Muscovy Duck Interferon-Alpha[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 523-527.
ZHU Hai-xia, WAN Chu-he, HUANG Yu, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei. Cloning and Sequence Analysis of Muscovy Duck Interferon-Alpha[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 523-527.
Citation:
ZHU Hai-xia, WAN Chu-he, HUANG Yu, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei. Cloning and Sequence Analysis of Muscovy Duck Interferon-Alpha[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 523-527.
Reverse transcription-polymerase chain reaction(RT-PCR)method was used to amplified the IFN- gene fragment from lung tissue of Muscovy duck,then cloned to pMD18-T vector,the positive clones were confirmed by PCR and restriction enzyme digestion,the obtained sequence were analyzed by the Lasergene v7.1 DNAStar software.The results showed that,the Muscovy duck IFN- gene encoding a 576 bp open reading frame(ORF),which was first reported in the world.The nucleotide had higher similarity ranging from 95.5% to 97.7% among different goose species,also had higher similarity ranging from 97.0% to 97.7% among different duck species;however,the ducks and geese IFN- gene had its' own phylogentic lineage,which means,the IFN- gene had strictly specificity in different species.
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