Application of micronucleus test technology to salt-tolerance evaluation in broadbean gepmplasm resources

The difference of root-tip cell micronucleus frequency and salt-sensitive index among 100 Vicia faba varieties were studied.Correlativity among micronucleus frequency,salt-sensitive index and relatively salt damage rate were analyzed by the relatively damage rate of seedlings under salt-stress.Micronucleus test technology was adapted to appraise salt-tolerance of Vicia faba,the Vicia faba micronucleus frequency were 8.3 to 15.3,the salt-sensitive index were 0.33 to 2.56 under 3.0% NaCl.Salt-tolerance of 100 Vacia faba varieties was divided into 5 grades by salt-sensitive index,among whith,3 in Ⅰ,9 in Ⅱ,18 in Ⅲ,32 in Ⅳ and 38 in Ⅴ.