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Volume 21 Issue 4
Apr.  2012
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HU Run-fang, XUE Zhu-zheng, TANG Yong-hui. RAPD analysis on Pleurotus nebrodensis (Inzengae) QuèL. strains[J]. Fujian Journal of Agricultural Sciences, 2006, 21(4): 346-349.
Citation: HU Run-fang, XUE Zhu-zheng, TANG Yong-hui. RAPD analysis on Pleurotus nebrodensis (Inzengae) QuèL. strains[J]. Fujian Journal of Agricultural Sciences, 2006, 21(4): 346-349.
shu

RAPD analysis on Pleurotus nebrodensis (Inzengae) QuèL. strains

  • 1.

    Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;

  • 2.

    Lianjiang Seed Company, Lianjiang, Fujian 350500, China

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  • Received Date: April 05, 2006
  • Revised Date: August 13, 2006
    Graphical Abstract
    • Abstract
  • Total genome DNA of P.nebrodensis mycelium was extracted by CTAB and the genetic relationships among 13 P.nebrodensis strains were analyzed by RAPD.11 primers of 80 random primers produced stable results.Among 66 bands obtained from 11 primers,50 bands were polymorphic bands which occupied 75.76% of the total amplified bands.Cluster analysis showed that the materials could be divided into 2 groups at the level of D=0.46,which evidently showed that the funnel-shaped fruitbody with a longer stem strains were grouped together,the horseshoe or palm-shaped fruitbody with a very short stem strains were grouped together.The cluster analysis of varieties revealed the relationships among P.nebrodensis strains.
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    • References (4)
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    陈文良,刘宇,王丽珍,等.不同配方培养料对白灵侧耳生长的影响[J].食用菌学报,2004,11(1):37-41.
    唐启义,冯光明.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002.
    曾东方,罗信昌.应用RAPD分析快速鉴定外生菌根蘑菇分离物的真伪[J].林业科学,2001,37(6):59-64.
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