SSCP polymorphism of the HSPA1A gene and its relationship with heat tolerant capacity of Jersey and Holstein cows

The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5’-flanking region of the HSPA1A gene.The PCR-SSCP products of primer showed polymorphisms and could be divided into four genotypes: AA,AB,AC,CC among 70 Jersey and Holstein cows.The ac genotypic cows showed higher heat tolerant capacity than those of AA,AB,and BB genotype.These mutation sites can be used as molecular genetic markers to assist the selection for anti-heat stress cows.