Specific proteins of "Wuhexuegan" and Citrus sinesis Osbeck at embryo development stage
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Graphical Abstract
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Abstract
The method of Coomassie brilliant blue was used to determine the protein content in embryos.It indicated that the protein content in "Wuhexuegan" was higher than that in Citrus sinesis Osbeck at the early stage of embryo development.In order to study the specific proteins of "Wuhexuegan" at the embryonic development stage,two-dimensional electrophoresis technique was employed.As a result,6 new proteins at different stages of "Wuhexuegan" embryo development and 4 specific proteins of "Wuhexuegan" that were different from Citrus sinesis Osbeck in the embryonic development stage were identified.The 4 proteins were P11(67.0kD,pI 5.1),P12(65.5kD,pI 5.4,P13(97.0kD,pI 5.8),and P14(64.3kD,pI 5.9).Therefore,it is suggested that these 4 specific proteins might play an important role in "Wuhexuegan" embryo differentiation and development.
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