Specific proteins of "Wuhexuegan" and <i>Citrus sinesis</i> Osbeck at embryo development stage

WANG Yu-ling; CHEN Qing-xi; CHEN Wei

Volume 22 Issue 2

Apr. 2012

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2007 > 22(2): 158-161

WANG Yu-ling, CHEN Qing-xi, CHEN Wei. Specific proteins of 'Wuhexuegan' and Citrus sinesis Osbeck at embryo development stage[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 158-161.

Citation:

WANG Yu-ling, CHEN Qing-xi, CHEN Wei. Specific proteins of "Wuhexuegan" and Citrus sinesis Osbeck at embryo development stage[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 158-161.

WANG Yu-ling, CHEN Qing-xi, CHEN Wei. Specific proteins of 'Wuhexuegan' and Citrus sinesis Osbeck at embryo development stage[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 158-161.

Citation:

WANG Yu-ling, CHEN Qing-xi, CHEN Wei. Specific proteins of "Wuhexuegan" and Citrus sinesis Osbeck at embryo development stage[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 158-161.

PDF( 1107 KB)

Specific proteins of "Wuhexuegan" and Citrus sinesis Osbeck at embryo development stage

1.
Department of Biological Science and Biotechnology, Zhangzhou Normal University, Zhangzhou, Fujian 363000, China;
2.
Horticultural College, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China;
3.
Plant Proteomics Laboratory of College of Life Science, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2007-02-10
Rev Recd Date: 2007-05-22
Publish Date: 2007-06-15

Abstract

Abstract

The method of Coomassie brilliant blue was used to determine the protein content in embryos.It indicated that the protein content in "Wuhexuegan" was higher than that in Citrus sinesis Osbeck at the early stage of embryo development.In order to study the specific proteins of "Wuhexuegan" at the embryonic development stage,two-dimensional electrophoresis technique was employed.As a result,6 new proteins at different stages of "Wuhexuegan" embryo development and 4 specific proteins of "Wuhexuegan" that were different from Citrus sinesis Osbeck in the embryonic development stage were identified.The 4 proteins were P11(67.0kD,pI 5.1),P12(65.5kD,pI 5.4,P13(97.0kD,pI 5.8),and P14(64.3kD,pI 5.9).Therefore,it is suggested that these 4 specific proteins might play an important role in "Wuhexuegan" embryo differentiation and development.
- Wuhexuegan,
- Citrus sinesis Osbeck,
- embryo development

FullText(HTML)

References(3)

References

[1]	SLOBODAN P GERHARD S,HELMUT Z,et al.Isoelectric focusing in serial immobilized pH gradient gels to improve protein separation in proteomic analysis[J].Electrophoresis,2005,26(16):3185-3190.
[2]	WOLFANG S,MICHAEL W,MOHAMMED M,et al.Towards validating a method for two-dimensional electrophoresis/silver staining[J].Electrophoresis,2005,26(12):2461-2469.
[3]	梁文裕,陈伟,宋瑞锋,等."乌龙岭"龙眼胚胎发育时期特异性蛋白质的变化[J].热带亚热带植物学报,2005,l3(3):229-232.