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ZHANG Feng, LI Peng, XU Hong-chuan, QI Jian-min, CHEN Wei. Application of protein electrophoresis on the seed identification of hybrid rice[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 235-240.
Citation: ZHANG Feng, LI Peng, XU Hong-chuan, QI Jian-min, CHEN Wei. Application of protein electrophoresis on the seed identification of hybrid rice[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 235-240.

Application of protein electrophoresis on the seed identification of hybrid rice

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  • Received Date: May 07, 2007
  • Revised Date: June 18, 2007
  • Salt-and alcohol-soluble proteins extracted from the seeds of four different hybrid rice and their three lines,as well as seven other F1 hybrid progenies were analyzed by means of SDS-PAGE.The results showed that the polymorphism of alcohol-soluble proteins was not significant enough for authenticity and purity assessment.However,the salt-soluble proteins had a high polymorphism with stable and specific bands.The experiments demonstrated that SDS-PAGE was a simple,rapid,effective and inexpensive method for the identification of hybrid rice seeds.
  • 凌建群,何祖华,马志超,等.水稻醇溶蛋白的多态性研究[J].中国水稻科学[J].1997,11(3):129-135.
    GEBRE H.Barley cultivar identification by Polyacrylamide gel electrop Horesis of hordein proteins:catalog of cultivars[J].Crop Sci,1986,26 (3):454-460.
    张春庆,贾继增.棉花种子蛋白多态性与品种鉴定方法的研究[J].中国农业科学,1998,31(4):16-19.
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