Application of protein electrophoresis on the seed identification of hybrid rice

Salt-and alcohol-soluble proteins extracted from the seeds of four different hybrid rice and their three lines,as well as seven other F1 hybrid progenies were analyzed by means of SDS-PAGE.The results showed that the polymorphism of alcohol-soluble proteins was not significant enough for authenticity and purity assessment.However,the salt-soluble proteins had a high polymorphism with stable and specific bands.The experiments demonstrated that SDS-PAGE was a simple,rapid,effective and inexpensive method for the identification of hybrid rice seeds.