• 中文核心期刊
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  • 中国科技核心期刊
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CHEN Zhen-hai, LIN Tian-long, YU Fu-song, SONG Tie-ying, GONG Hui, FANG Qin-mei, YANG Jin-xian. Establishment of recombinant adenoviruses for expressing PRRSV GP2a/GP2b,GP3,GP4,GP5,and M proteins[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 221-226.
Citation: CHEN Zhen-hai, LIN Tian-long, YU Fu-song, SONG Tie-ying, GONG Hui, FANG Qin-mei, YANG Jin-xian. Establishment of recombinant adenoviruses for expressing PRRSV GP2a/GP2b,GP3,GP4,GP5,and M proteins[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 221-226.

Establishment of recombinant adenoviruses for expressing PRRSV GP2a/GP2b,GP3,GP4,GP5,and M proteins

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  • Received Date: July 19, 2007
  • Revised Date: August 12, 2007
  • GP2a/GP2b-3,GP3,GP4,GP5,and M protein gene fragments of porcine reproductive and respiratory syndrome virus FJ-1a strain were amplified by RT-PCR and cloned onto shuttle vector pDC316.After co-transfection of one of the five recombinant shuttle vectors and the helper plasmid into 293 cells,recombinant adenoviruses Ad-GP2a/GP2b-3,Ad-GP3,Ad-GP4,Ad-GP5 and Ad-M were obtained.The titers of the five adenoviurses were 107.7-109.0 TCID50·mL-1.Among them,the Ad-GP5 titer was the lowest,at 107.7 TCID50·mL-1.The genome stability was determined by PCR detection of heterologous gene fragments in recombinant adenoviruses at 3rd,6th and 9th passages.In addition,when the recombinant adenoviruses were infected with the 293 cells,mRNA transcription of heterologous genes were confirmed by RT-PCR test.The establishment of recombinant adenoviruses for expressing PRRSV envelope proteins would provide a basic for further study on PRRSV genetic vaccine and the functions of the proteins.
  • MEULENBERG J J,HULST M M,DE MEIJER E J,et al.Lelystad virus,the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS),is related to LDV and EAV[J].Virology,1993,192 (1):62-72.
    MARDASSI H,MASSIE B,DEA S.Intrscellular synthesis,processing,and transport of proteins encoded by ORFs 5 to 7 of porcine reproductive and respiratory syndrome virus[J].Virology,1996,221 (1):98-112.
    JIANG W,JIANG P,LI Y,et al.Recombinant adenovirus expressing GP5 and M fusion proteins of porcine reproductive and respiratory syndrome virus induce both humoral and cellmediated immune responses in mice[J].Vet Immunol Immunopathol,2006,113 (1-2):169-180.
    WU W H,FANG Y,FARWELL R,et al.A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b[J].Virology,2001,287 (1):183-191.
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