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WU Wen-shan, XIE Qing-qing, ZHENG Cui-fang, ZHU Xiao-dong, LIN Wei, HUANG Mei-li. Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 410-413.
Citation:
WU Wen-shan, XIE Qing-qing, ZHENG Cui-fang, ZHU Xiao-dong, LIN Wei, HUANG Mei-li. Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 410-413.
WU Wen-shan, XIE Qing-qing, ZHENG Cui-fang, ZHU Xiao-dong, LIN Wei, HUANG Mei-li. Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 410-413.
Citation:
WU Wen-shan, XIE Qing-qing, ZHENG Cui-fang, ZHU Xiao-dong, LIN Wei, HUANG Mei-li. Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 410-413.
A method of extraction and assay for the pectinesterase were investigated and proposed as follows: the best concentration of NaCl in extracting mixtrure is 5%,the best extracting temperature is 30℃,the best extracting time is 24 hours,the best conservating time is 24 hours,the best concentration of NaCl in mixture is 0.6% to 1.0%,and maintain the pH on 7.0.Between 30-50℃,the pectinesterase in achenes of Ficus awkeotsang has a good thermal stability.
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