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SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.
Citation:
SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.
SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.
Citation:
SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.
Eight bacterial isolates,which originated from paddy fields in Fujian,were analyzed using 16SrDNA-PCR-DGGE.Through sequencing and Blast analysis,9 N2-fixing bacteria strains were believed to belong to Mesorhizobium sp.,Sphingomonas sp.,Rhizobium sp.,Bradyrhizobium sp.,Klebsiella sp.,Burkholderia cepacia,Pseudomonas sp.,Nocardioides and Rhodococcus sp.,due to their high homology with the related accessions in Genbank.
WATANABE K,KIDAMA Y,HARAYAMA S.Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting[J].Microbiol Methods,2001,44(3):253-262.
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