PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria
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Graphical Abstract
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Abstract
Eight bacterial isolates,which originated from paddy fields in Fujian,were analyzed using 16SrDNA-PCR-DGGE.Through sequencing and Blast analysis,9 N2-fixing bacteria strains were believed to belong to Mesorhizobium sp.,Sphingomonas sp.,Rhizobium sp.,Bradyrhizobium sp.,Klebsiella sp.,Burkholderia cepacia,Pseudomonas sp.,Nocardioides and Rhodococcus sp.,due to their high homology with the related accessions in Genbank.
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