Study on the selectivity of epiphytic lactic acid bacteria to the carbon sources in the medium

CHEN Xinzhu; DONG Zhaoxia; ZHANG Jianguo

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CHEN X Z, DONG Z X, ZHANG J G. Study on the selectivity of epiphytic lactic acid bacteria to the carbon sources in the medium [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：1−10

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CHEN X Z, DONG Z X, ZHANG J G. Study on the selectivity of epiphytic lactic acid bacteria to the carbon sources in the medium [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：1−10

CHEN X Z, DONG Z X, ZHANG J G. Study on the selectivity of epiphytic lactic acid bacteria to the carbon sources in the medium [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：1−10

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Study on the selectivity of epiphytic lactic acid bacteria to the carbon sources in the medium

CHEN Xinzhu^{1, 2
,},
DONG Zhaoxia¹,
ZHANG Jianguo^{1
,
,}

1.
Department of Grassland Science, South China Agricultural University, Guangzhou, Guangdong　510642, China
2.
Institute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory of Animal Genetics and Breeding, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

Received Date: 2023-11-28
Rev Recd Date: 2024-02-18

Available Online: 2024-06-26

Abstract

Abstract

In order to explore the selectivity of different lactic acid bacteria (LAB) to the carbon source of medium, 16 representative LAB were isolated from forage crops as the research object were selected. In the MRS broth medium, glucose, sucrose, fructose, xylose, raffinose and mixed sugars (raffinose-glucose-fructose-sucrose-xylose) were used as carbon sources for the 16 strains. There were 3 replicates for each strain, and a blank control (without inoculation) for each sugar treatment. After 24 hours of inoculation’, the high-performance liquid chromatography was used to determine the contents of residual carbon source and lactic acid in the broth for each treatment. The results of the 16 strains showed that: (1) Sucrose was the preferred carbon source for the growth of most strains; there were differences in the selection of glucose, fructose and xylose among different strains. Lactobacillus fermentum LbF-WM preferred raffinose and xylose, and Leuconostoc pseudomesenteroides LeP-IR preferred xylose. (2) The utilization rate of sucrose reached 100% by Lactobacillus brevis LbR-EG and Leuconostoc citreum LeC-IR, Lb. fermentum LbF-WM and Lactobacillus reuteri LbR-EG used fructose, Lb. fermentum LbF-WM, Lb. reuteri LbR-EG and Le. pseudomesenteroides LeP-IR used raffinose. The utilization rate of glucose by Enterococcus faecium EF-KG, Lb. brevis LbB-KG, Lactobacillus pentosus LbPe-EG and Lactococcus lactis LcL-SC were higher than that of other carbon sources. (3) The lactic acid yield was higher by E. durans EG-IR using glucose and mixed sugar, Lb. pentosus LbPe-EG used fructose, Leuconostoc mesenteroides LeM-IR fermented raffinose and mixed sugar, Lb. pentosus LbPe-EG useed sucrose, Lb. fermentum LbF-WM used xylose, and Lactococcus garvieae LcG-IR used mixed sugar. (4) The lactic acid production was more efficient by E. durans ED-IR using glucose, Lb. plantarum LbPl-IR, Le. mesenteroides LeM-IR and Lb. pentosus LbPe-EG used fructose, Lb. plantarum LbPl-IR and Le. mesenteroides LeM-IR used raffinose, Lb. pentosus LbPe-EG, Lb. plantarum LbPl-IR and E. durans ED-IR used sucrose, and Le. pseudomesenteroides LeP-IR used xylose, Lc. garvieae LcG-IR and E. durans EG-IR used mixed sugars. The lactic acid production efficiency of Lb. plantarum LbPl-IR used 5 kinds sugars was more than 80%. In summary, the different strains had different sugar utilization, acid production and acid production efficiency in the same sugar source, and the same strain fermented different sugars also had different sugar utilization, acid production and acid production efficiency. In view of the types of LAB distributed on forage crops, selecting the most suitable carbon source would benefit to more rapid and large-scale production of lactic acid, lowering the pH value, and better preserving the nutrients of silage.
- epiphytic lactic acid bacteria,
- carbon source,
- glucose,
- sucrose,
- selectivity

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References(27)

References

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