Simultaneous Determination of Sulfadiazine and Sulfamethoxazole Residues in Muscle of European Eels (Anguilla anguilla)by HPLC

A high performance liquid chromatographic method was developed for the simultaneous determination of sulfadiazine and sulfamethoxazole in European eels (Anguilla anguilla) muscle. Homogenized tissue were extracted with 10% sodium sulfate and acetonitrile- Chloroform(10∶1), the extract was concentrated by nitrogen, and redissolved into 2 mL mobile phase and defatted with n-hexane. Sulfadiazine and sulfamethoxazole were separated on Eclipse XDB-C18 Column with mobile phases containing acetonitrile and 0.05 molL-1 ammonium dihydrogen phosphate (20∶80) and finally determined by Multiple Wavelength Detector (MWD). The detection limit of sulfadiazine and sulfamethoxazole were 4 gkg-1and 8 gkg-1.In the range of 0.01~5.0 gkg-1, the mean recoveries were more than 80%. The intraday and interday precisions were less than 5%. It showed that the method meet the conventional residue testing requirements.