A preliminary study on explant tissue culture and in vitro plantlet preservation for tea

The callus induction,subculture and regeneration of tea explants of seeds,cotyledons,leaves,roots and short stems for Camellia.sinensis cv.Fudingdabai were conducted to determine material and means for preserving tea cultivars.The results showed that(a) the tea leaves were the best explant for inducing calli using N6+2,4-D at the concentration of 2.0 mgL-1 as the induction medium;(b) a great deal of calli could be obtained by application of the multiple-subculture technique;and(c) the color of most calli were green on the 20 regeneration media tested,however,roots could be regenerated from the calli only on some of the media.