• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊
Volume 25 Issue 6
Nov.  2011
Turn off MathJax
Article Contents
Abstract
References
Related Articles
YU Zhi-xiong, CHEN Ming-xian, YUAN Ya-fang, WU Qiong-feng. Optimization of ISSR reaetion system in pitaya[J]. Fujian Journal of Agricultural Sciences, 2010, 25(6): 711-715.
Citation: YU Zhi-xiong, CHEN Ming-xian, YUAN Ya-fang, WU Qiong-feng. Optimization of ISSR reaetion system in pitaya[J]. Fujian Journal of Agricultural Sciences, 2010, 25(6): 711-715.
shu

Optimization of ISSR reaetion system in pitaya

  • 1.

    Fujian Vocational College of Agriculture, Fuzhou, Fujian 350119, China;

  • 2.

    Horticultural Department, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China

More Information
  • Received Date: August 22, 2010
  • Revised Date: October 21, 2010
    Graphical Abstract
    • Abstract
  • In order to establish a stable ISSR-PCR reaction system for pitaya,a single factor experiment and orthogonal method was applied for the optimization.The results showed that the optimum concentrations of different components in 20 μL reaction system were 2.0 μL 10×Buffer(with Mg2+),20.0 ng template DNA,0.30 mmolL-1 dNTP,0.35 μmolL-1 primer and 1.5 U Taq DNA polymerase.The optimized annealing temperature was 54.0℃ for the primer(AC)8T.The established ISSR-PCR system was tested on 6 pitaya samples with satisfactory performance.
    • FullText(HTML)
    • References (1)
  • 齐清琳.不同品种火龙果引种栽培比较实验[J].福建林业科技,2004,31(4):48-50.
    • Related Articles

  • Related Articles

    [1]WU Mingji, LIU Huaqing, CHEN Jianmin, FU Yanping, YANG Shaohua, SONG Yana, LIN Yan, WANG Feng. Critical Sterility-inducing Temperature of Rice Affected by tms5 Mutation Site[J]. Fujian Journal of Agricultural Sciences, 2023, 38(7): 761-771. DOI: 10.19303/j.issn.1008-0384.2023.07.001
    [2]WANG Yan, ZHANG Chaolin, WANG Juan, DENG Yue, SU Xiaorui, TAN Feifei, TIAN Kegong. Effects of Vectors on Transient Expression of GFP in CHO Cells[J]. Fujian Journal of Agricultural Sciences, 2020, 35(8): 851-856. DOI: 10.19303/j.issn.1008-0384.2020.08.006
    [3]DENG Ze-qin, HUANG Xian-bo, LIN Cheng-bao, TANG Jiang-xia, YE Yang-dong, SU Rong-li, LIANG Shui-jin. Improvement of Disease-resistance of Restorer Rice Lines by Transducing Genes Pi9 onto Sanming Dominant Male Sterile Lines[J]. Fujian Journal of Agricultural Sciences, 2020, 35(1): 6-12. DOI: 10.19303/j.issn.1008-0384.2020.01.002
    [4]LIN Yan, LIU Hua-qing, FU Yan-ping, WU Ming-ji. TALEN-mediated Editing of Photoperiod-temperature-sensitive Male Sterility PMS3 Gene in Rice[J]. Fujian Journal of Agricultural Sciences, 2019, 34(4): 381-386. DOI: 10.19303/j.issn.1008-0384.2019.04.001
    [5]WU Ming-ji, LIN Yan, LIU Hua-qing, CHEN Jian-min, FU Yan-ping, YANG Shao-hua, WANG Feng. Development of Thermo-Sensitive Male Sterile Rice with CRISPR/Cas9 Technology[J]. Fujian Journal of Agricultural Sciences, 2018, 33(10): 1011-1015. DOI: 10.19303/j.issn.1008-0384.2018.10.001
    [6]Li-bin HE, Fang CHEN, Zhi-huang ZHU, Min-ning MA, He-chang ZHANG, Sun-long WU, Chen ZHOU. Molecular Phylogeny Determined by Mitochondrial Genes 16S rRNA, COX1 and Cytb of Eleven Anemonefish Species[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 230-235. DOI: 10.19303/j.issn.1008-0384.2018.03.003
    [7]XIONG Gui-hong, LIU Xiao-juan, YANG Liang, WU Zu-jian. Subcellular Localization Analysis of Rice Protein Kinase Gene OsCIPK5 and Generation of Its RNAi Transgenic Plants[J]. Fujian Journal of Agricultural Sciences, 2017, 32(4): 353-358. DOI: 10.19303/j.issn.1008-0384.2017.04.001
    [8]LÜ Xin, LIU Lan-ying, CHEN Li-hua, LI Yue-ren, LIN Bi-jiao. Composition of Bacterial Community at Lake Zuohai Determined by PCR-Denaturing Gradient Gel Electrophoresis[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 986-992. DOI: 10.19303/j.issn.1008-0384.2016.09.017
    [9]LAN Hua-xiong. Breeding of low-temperature sensitive genic male sterile Fulong S2 by means of blast resistance and high-altitude ecological pressure selection[J]. Fujian Journal of Agricultural Sciences, 2009, 24(4): 296-303.
    [10]Zhang Shougang, Xu Xuming, Zhuo Wei, Yang Tengbang, Huang Xianbo, Fan Zhujun. Breeding and Selection of Mingguang 87s A Photosensitive Male Sterile of Indica Rice[J]. Fujian Journal of Agricultural Sciences, 1998, 13(1): 15-20.

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (2329) PDF downloads (991) Cited by()
    Related

    Copyright © 2018 Fujian Journal of Agricultural Sciences, All Rights Reserved. 闽ICP备19004781号-1 网安备：35010046024-23001

    Address: 247 Wusi Road, Fuzhou City China Pos: 350003 Tel: 0591-87869455 E-mail: fjnyxb@163.com

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return