Isolation and gene sequence analysis of PRRSV-FJ07A strain's structural proteins

PRRSV-FJ07A strain was isolated and identified.The virus was tested on the Marc-145 cell and subjected to RT-PCR,TCID50,gene sequencing of structural protein and an animal challenge test.The results showed that PRRSV-FJ07A strain belonged to the family of the North American genotype of PRRSV.Its TCID50 was 10-6.5.ml-1.Compared with VR-2332 strain,the homogeneities of nucleotide and amino acid were 89.2%-95.8% and 88.1%-96.2%,respectively,90.1%-99.7% and 88.3%-100.0%,respectively,compared with HUN4 strain,and 63.7%-70.0% and 59.6%-78.9%,respectively,compared to and LV strain.After 3 to 7 days PRRSV-FJ07A innoculation,the 35-day-old piglets showed negative serum antibody of PRRSV and appeared with clinical syndromes of PRRSV infection.The gene sequence of PRRSV-FJ07A has been submitted to Genbank issued with the number of GI: 283100314.