• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊
Volume 25 Issue 3
Nov.  2011
Turn off MathJax
Article Contents
Abstract
References
Related Articles
OUYANG Sui-dong, LIN Feng-qiang, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, ZHU Xiao-li, HU Qi-lin, CHEN Shao-ying. σC gene expresson of muscovy duck reovirus MW9710 strain in Pichia pastoris[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 241-244.
Citation: OUYANG Sui-dong, LIN Feng-qiang, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, ZHU Xiao-li, HU Qi-lin, CHEN Shao-ying. σC gene expresson of muscovy duck reovirus MW9710 strain in Pichia pastoris[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 241-244.
shu

σC gene expresson of muscovy duck reovirus MW9710 strain in Pichia pastoris

  • 1.

    Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;

  • 2.

    Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

More Information
  • Received Date: February 02, 2010
  • Revised Date: May 16, 2010
    Graphical Abstract
    • Abstract
  • The σC gene of DRVMW9710 was inserted into pPIC9K to construct a recombinant secretory vector,pPIC9K-σC.Then,pPIC9K-σC was transformed into E. coli DH5α.The recombinant vector was identified by PCR,enzymatic analysis and sequencing.The positive plasmid was linearized by Sac I and transformed into GS115 by electroporation.The insert was integrated into genomic DNA of Pichia pastoris by means of homologous recombination.The recombinants with multiple integrated copies of σC were screened using gradient concentration G418,and inducted with methanol.The results of SDS-PAGE and Western-blot showed that the highly expressed protein had an immunoreaction property.
    • FullText(HTML)
    • References (2)
  • 欧阳岁东,胡奇林,林锋强,等.番鸭呼肠孤病毒MW9710株δC蛋白基因的克隆和序列分析[J].中国预防兽医学报,2006,28(5):510-513.
    GALL-RECULE G L,CHERBONNEL M.Molecular characterization and expression of the S3 gene of muscovy duck reovirus strain 89026[J].J Gen Girology,1999,80:195-203.
    • Related Articles

  • Related Articles

    [1]Zhang Gongliang, Li Yi, Wei Yuanyuan, Zhao Haicheng, Chen Kexin, Huang Jiqing, Liu Menghong, Li Hongyu. Principal Component Analysis and Comprehensive Evaluation on Drought Resistance-related Traits of Rice for Cultivation in Cold Regions[J]. Fujian Journal of Agricultural Sciences, 2020, 35(8): 811-819. DOI: 10.19303/j.issn.1008-0384.2020.08.001
    [2]WANG Hong-mei, YANG Sen, HUANG Hai-rui, DING Li-mei, FEI Yue, WU Qian. Ornamental Property of Cultivated Varieties of Hydrangea paniculata[J]. Fujian Journal of Agricultural Sciences, 2020, 35(3): 286-294. DOI: 10.19303/j.issn.1008-0384.2020.03.007
    [3]LIN Wen-lei, LÜ Mei-qin, LI Ming-song, KANG Rong-rong, ZENG Hong-ying, YAO Wen, CAI Jin-ling. Principal Component Analysis and Cluster Analysis of 39 Spring Soybean Germplasm Resources[J]. Fujian Journal of Agricultural Sciences, 2018, 33(10): 1016-1022. DOI: 10.19303/j.issn.1008-0384.2018.10.002
    [4]DU Gong-fu, ZHAN Yuan-feng, LIU Zi-ji, HE Huang, QI Zhi-qiang, DANG Xuan-min. Cluster Analysis on Resistance of Watermelon Rootstocks against Meloidogyne incognita Infection[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1354-1358. DOI: 10.19303/j.issn.1008-0384.2017.012.015
    [5]CHEN Yi-ting, LAI Rui-lian, FENG Xin, GAO Min-xia, ZHANG Chang-he, WU Ru-jian. Codon Usage Bias of Chalcone Synthase Gene CHS in Kiwifruit[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1213-1217. DOI: 10.19303/j.issn.1008-0384.2017.011.009
    [6]YE Xin-ru, ZHU Hai-sheng, WEN Qing-fang, LI Zu-liang, LIU Jian-ting, WANG Bin, CHEN Min-dong, ZHANG Qian-rong, HUANG Ai-ping. Principal Components and Cluster Analyses on Luffa Germplasms[J]. Fujian Journal of Agricultural Sciences, 2017, 32(7): 703-709. DOI: 10.19303/j.issn.1008-0384.2017.07.003
    [7]YANG Wang-xing, ZHUO Wei, MA Bin-lin, ZOU Wen-guang, WEI Xin-yu, YANG Teng-bang, ZHANG Shou-gang, Xu Xu-ming. Comparison and Cluster Analysis on Photosynthetic Characteristics and Water Usage Efficiency of Rice Germplasms[J]. Fujian Journal of Agricultural Sciences, 2017, 32(3): 248-252. DOI: 10.19303/j.issn.1008-0384.2017.03.004
    [8]YAO Jin-ai, CAI Hong-jiao, SHI Niu-niu, GAN Lin, YANG Xiu-juan, CHEN Fu-ru. Genetic Relationship among Banana (Musa spp.) Germplasms Revealed by ISSR Analysis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(1): 37-42.
    [9]HU Run-fang, XUE Zhu-zheng, TANG Yong-hui. RAPD analysis on Pleurotus nebrodensis (Inzengae) QuèL. strains[J]. Fujian Journal of Agricultural Sciences, 2006, 21(4): 346-349.
    [10]WEN Qing-fang, LI Da-zhong, ZHU Hai-sheng, HUANG Ke. RAPD analysis of genetic diversity on balsam pear materials (Momordica charantia L. )[J]. Fujian Journal of Agricultural Sciences, 2005, 20(3): 185-188.

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (3137) PDF downloads (587) Cited by()
    Related

    Copyright © 2018 Fujian Journal of Agricultural Sciences, All Rights Reserved. 闽ICP备19004781号-1 网安备：35010046024-23001

    Address: 247 Wusi Road, Fuzhou City China Pos: 350003 Tel: 0591-87869455 E-mail: fjnyxb@163.com

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return