Optimization of culture medium and conditions for adventitious bud proliferation of papaya, "Zhanghong"

Factors affecting the multiplication of the new papaya variety,"Zhanghong",in tissue culture were studied. The orthogonal analysis indicated that,among the four factors investigated,BA and KT exerted the greatest effect on the stem growth,followed by sucrose and NAA. Result from the single factor experiments showed that the optimal media for the culture multiplication was MS+0.6% BA+0.2% KT+0.15% NAA+4% sucrose,the temperature was 25℃,and the illumination was 2000 lx.