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Volume 25 Issue 2
Nov.  2011
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WU Yu-fen, ZHOU Xian-zhi, CHEN Sheng, CHEN Yang, ZHAO Yi-jie. Phylogeny of Cucurbita Based on ITS sequence data[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 170-175.
Citation: WU Yu-fen, ZHOU Xian-zhi, CHEN Sheng, CHEN Yang, ZHAO Yi-jie. Phylogeny of Cucurbita Based on ITS sequence data[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 170-175.
shu

Phylogeny of Cucurbita Based on ITS sequence data

  • 1.

    Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;

  • 2.

    Fuzhou Institute of Agricuhural Sciences, Fuzhou, Fujian 350018, China

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  • Received Date: January 04, 2010
  • Revised Date: March 31, 2010
    Graphical Abstract
    • Abstract
  • The relationships and differences among the species,Cucurbita,pepo and ficifofia (Cucurbitaceae:Cucurbita),were examined using data from the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA. The ITS sequences were obtained from Cucurbita,pepo and ficifofia 3 species. The results showed that,within Cucurbita,ITS1 was 188-191bp (C. Cucurbita,C. pepo and C. ficifofia) and ITS2 ranged from 251-263bp (C. Cucurbita,C. pepo and C. ficifofia). There were 33 variable positions (17.55%) and 24 informative sites (12.77%) within Cucurbita found in the ITS1 sequences,while 45 variable positions (17.93%) and 31 informative sites (12.35%) found in the ITS2 sequences.The G+C content of ITS1 was 51.60% to 54.50% and that of ITS2 ranged from 53.78% to 56.98%.The resulting trees from the ITS1 and ITS2 sequences showed that the relationship between C. Cucurbita and C. pepo was closer than that between C. Cucurbita and C. ficifofia.
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    • References (4)
  • 赵福宽,李海英,赵晓萌.南瓜种质资源亲缘关系的RAPD分析[J].分子植物育种,2006,4 (3):45-50.
    张天明,屈东玉,王长林,等.南瓜属4个栽培种亲缘关系的AFLP分析[J].中国蔬菜,2006(1):11-14.
    HSIAO C,CHATTERTON N J,ASAY K H,et al.Phylogenetic relationships of the monogenomic species of the wheat tribe,Triticeae(Poaceae),inferred from nuclear rDNA (internal transcribed spacer) sequences[J].Genome,1995,38 (2):211-223.
    戴小军,梁满中,陈良碧.栽培稻种内核糖体基因的ITS序列比较研究[J].作物学报,2007,33 (11):1874-1878.
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