Green fluorescent protein gene transformation on <i>Fusarium oxysporum</i> f. sp. <i>niveum</i> strain, FOV-135

XIAO Rong-feng; ZHU Yu-jing; LI Yan-dan; HUANG Su-fang; LIU Bo

Volume 24 Issue 6

Nov. 2011

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Article Navigation > Fujian Journal of Agricultural Sciences > 2009 > 24(6): 521-524

XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo. Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 521-524.

Citation:

XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo. Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 521-524.

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Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135

1.
Institute of Agrobiological Resouces, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2009-11-28
Rev Recd Date: 2009-12-31
Publish Date: 2009-12-15

Abstract

Abstract

The gene coding for green fluorescent protein(GFP) was successfully transferred to Fusarium oxysporum f.sp.niveum strain,FOV-135,by using the protoplast-mediated genetic transformation system.The transformants were stable after five successive subcultures.Four to six stable transformants could be obtained from one microgram of plasmid DNA.The PCR amplification confirmed that the gfp gene had been transformed to FOV-135.
- Fusarium oxysporum f.sp.niveum,
- green fluorescent protein,
- protoplast-mediated genetic transformation system

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References(2)

References

[1]	NAHALKOVA J,FATEHI J.Red fluorescent protein (DsRed2) as a novel reporter in Fusarium oxysporum f.sp.lycopersici[J].FEMS Microbiology Letters,2003,225:305-309.
[2]	NONOMURA T,TAJIMA H,KITAGAWA Y,et al.Distinguishable staining with neutral red for GFP-marked and GFP-nonmarked Fusarium oxysporum strains simultaneously colonizing root surfaces[J].Journal of General Plant Pathology,2003,69:1,45-48.