• 中文核心期刊
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XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo. Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 521-524.
Citation: XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo. Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 521-524.

Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135

  • The gene coding for green fluorescent protein(GFP) was successfully transferred to Fusarium oxysporum f.sp.niveum strain,FOV-135,by using the protoplast-mediated genetic transformation system.The transformants were stable after five successive subcultures.Four to six stable transformants could be obtained from one microgram of plasmid DNA.The PCR amplification confirmed that the gfp gene had been transformed to FOV-135.
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