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Volume 24 Issue 6
Nov.  2011
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XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo. Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 521-524.
Citation: XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo. Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 521-524.
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Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135

  • Institute of Agrobiological Resouces, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

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  • Received Date: November 27, 2009
  • Revised Date: December 30, 2009
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    • Abstract
  • The gene coding for green fluorescent protein(GFP) was successfully transferred to Fusarium oxysporum f.sp.niveum strain,FOV-135,by using the protoplast-mediated genetic transformation system.The transformants were stable after five successive subcultures.Four to six stable transformants could be obtained from one microgram of plasmid DNA.The PCR amplification confirmed that the gfp gene had been transformed to FOV-135.
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    • References (2)
  • NAHALKOVA J,FATEHI J.Red fluorescent protein (DsRed2) as a novel reporter in Fusarium oxysporum f.sp.lycopersici[J].FEMS Microbiology Letters,2003,225:305-309.
    NONOMURA T,TAJIMA H,KITAGAWA Y,et al.Distinguishable staining with neutral red for GFP-marked and GFP-nonmarked Fusarium oxysporum strains simultaneously colonizing root surfaces[J].Journal of General Plant Pathology,2003,69:1,45-48.
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