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Volume 24 Issue 3
Nov.  2011
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SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, CHEN Zhen, PENG Chun-xiang, HUANG Yu. Development of PCR assay for detecting avian Pasteurella multocida[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 201-204.
Citation: SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, CHEN Zhen, PENG Chun-xiang, HUANG Yu. Development of PCR assay for detecting avian Pasteurella multocida[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 201-204.
shu

Development of PCR assay for detecting avian Pasteurella multocida

  • 1.

    Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;

  • 2.

    Fujian Animal Diseases Control Techology Development Center, Fuzhou, Fujian 350013, China

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  • Received Date: April 11, 2009
  • Revised Date: April 25, 2009
    Graphical Abstract
    • Abstract
  • To establish a specific and sensitive detection methodology for avian Pasteurella multocida infection,PCR was applied.The 460 bp of the specific fragments was amplified from avian P.multocida genome.None could be obtained from Escherichia coli,Riemerella anatipestifer,Salmonella,Staphyloccocus aureus,Avian paramyxovirus type 1,Muscovy duck retrovirus or genome of duck.The sensitivity test indicated that a minimum of 1ngμL-1 of avian P.multocida genome DNA could be detected by the newly developed PCR method.
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    • References (1)
  • ROCKE T E,SMITH S R,MIYAMOTO A,et al.A serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1[J].Avian Diseases,2002,46(2):370-377.
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