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Volume 24 Issue 3
Nov.  2011
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WANG Xi-long, GONG Hui, YANG Jin-xian, LIN Tian-long. Purification of Anguilla anguilla serum immunoglobulin by euglobulin precipitation[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 197-200.
Citation: WANG Xi-long, GONG Hui, YANG Jin-xian, LIN Tian-long. Purification of Anguilla anguilla serum immunoglobulin by euglobulin precipitation[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 197-200.
shu

Purification of Anguilla anguilla serum immunoglobulin by euglobulin precipitation

  • 1.

    Life Science Department of Fujian Normal University, Fuzhou, Fujian 350108, China;

  • 2.

    Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

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  • Received Date: April 14, 2009
  • Revised Date: May 10, 2009
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    • Abstract
  • The purification of serum immunoglobulin from the European eel(Anguilla anguilla) was carried out by means of euglobulin as well as ammonium sulfate precipitation.Purified by euglobulin precipitation,the resultant material analyzed by SDS-PAGE showed two distinct protein bands,indicating a higher purity of the A.anguilla serum immunoglobulin than that obtained by ammonium sulfate precipitation.The ELISA analysis showed that the serum immunoglobulin purifid by euglobulin precipitation had a higher antibody activity than that purified by ammonium sulfate precipitation.Furthermore,the Western-blot antibody activity test also yielded a positive result on the serum immunoglobulin purified by euglobulin precipitation.
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    • References (2)
  • 张福淼,杨桂文,李国田,等.鲈血清免疫球蛋白分离纯化及部分特性分析[J].动物学杂志,2004,39(5):9-12.
    鄢庆枇,韩一凡,高天翔,等.大黄鱼血清IgM纯化及其兔抗血清的制备[J].中国水产科学,2006,13(3):475-479.
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