Induced high-frequency plant regeneration of oncidium using its multiple shoots

Shoot tips of Oncidium were used as the explants to study the effect of some key factors on the induction,propagation and rooting of multiple shoots in Oncidium.The factors studied included the culture media(i.e.,MS,1/2MS and 1/2 N6),phytohormone(i.e.,6-BA and NAA) application and culture conditions(i.e.,organic additives,sugar and culture stages).From the results it was hoped that the core technology of Oncidium regeneration using its multiple shoots could be established.It was found that(a) the medium containing 1/2MS + 6-BA2.0mg•L-1 + NAA 0.1mg•L-1 was superior for the induction of multiple shoots from shoot tips,with a rate of 84.6%;(b) preferable propagation of multiple shoots was achieved on the medium containing MS + 6-BA 2.0mg•L-1 + NAA 0.1mg•L-1 + sugar 30g•L-1,with a 5.5 propagation coefficient;(c) test-tube plantlets of 2.0-3.0cm height were the optimal culture materials for the propagation;and(d) 100% rooting rate was obtained on the medium containing 1/2MS + IBA 0.5mg•L-1 + apple juice 100g•L-1+ activated carbon 0.5g•L-1 + sugar 20g•L-1.