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Volume 24 Issue 1
Nov.  2011
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SUN Fa-liang, DIAO You-xiang, LIU Yue-zhu, SUN Ning, MA Yan-fang. Synthesis and identification of florfenicol antigen[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 24-28.
Citation: SUN Fa-liang, DIAO You-xiang, LIU Yue-zhu, SUN Ning, MA Yan-fang. Synthesis and identification of florfenicol antigen[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 24-28.
shu

Synthesis and identification of florfenicol antigen

  • 1.

    College of Animal Science and Technology, Shandon Agricultural University, Tai'an, Shandong 271000, China;

  • 2.

    Liding Biotechnology Institute of Shandong Province, Ji'nan, Shandong 250001, China

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  • Received Date: July 29, 2008
  • Revised Date: October 12, 2008
    Graphical Abstract
    • Abstract
  • The immunogen,FFC-HS-BSA(florfenicol-bovine serum albumin),and the coating antigen FFC-HS-OVA(florfenicol-ovalbumin)were synthesized by using the mixed anhydride method.The man-made antigens were identified and certified by UV absorption,SDS-PAGE electrophoresis and animal immunization test.The results showed the process was successful.All antigens made had the correct haptens and carrier proteins.
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    • References (5)
  • HORSBERG T E,MARTINSEN B,VARMA K J.The disposition of 14C-florfenicol in Atlantic salmon[J].Aquaculture,1994,122:97-106.
    RUSSEL H A.Liquid Chromatographic Analysis of Chloramphenicol in Bovine Muscles[J].Chroma-tographia,1978,11:341-343.
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